Dear the Editor,
Among patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),
up to 20% develop a severe form of coronavirus disease 2019 (COVID-19) with dyspnea
and hypoxia, and one-quarter of those patients develop acute respiratory distress
syndrome (ARDS) in a median of 2.5 days, with a mortality rate of up to 50% (range,
16–78%) [1–4]. Effective treatment is thus urgently needed.
Huang et al. [5] reported that the severity of COVID-19 is associated with increased
levels of inflammatory cytokines such as IL-1, IL-6, and tumor necrosis factor-α (TNFα),
which resembles the cytokine profile observed in cases of secondary hemophagocytic
lymphohistiocytosis (sHLH) and macrophage activation syndrome (MAS) [6]. This is supported
by a recent report by Mehta et al. [7] in which all 35 patients with severe COVID-19
showed hemophagocytosis on bone marrow aspirates, cytopenia of two or more lineages,
and increased serum ferritin levels (≥ 2000 ng/mL). Control of hypercytokinemia is
key to successfully treating sHLH/MAS. However, the effectiveness of cytokine blocking
with anti-IL-1 and anti-IL-6 on sHLH/MAS has been limited [6].
The most common trigger for sHLH/MAS is a viral infection, which stimulates macrophages
to release inflammatory cytokine and perform phagocytosis of virus-infected cells.
Cytotoxic T lymphocytes (CTLs) are subsequently activated through their antigen-presenting
function, and hypercytokinemia occurs, resulting in virus-infected cells undergoing
perforin-mediated cell lysis. Finally, the activated CTLs selectively eliminate the
activated macrophages, and sHLH/MAS naturally resolves [8]. However, unlike other
virus infections, Epstein-Barr virus (EBV) initially activates CTLs to induce hypercytokinemia
directly and indirectly, thereby allowing for prolonged antigen presentation by macrophages,
which causes CTLs to fail to eliminate activated macrophages; this lack of normal
feedback regulation results in excessive macrophage activity and hypercytokinemia,
thereby leading to the development of organ damage, cytopenia, and coagulopathy [8]
(Fig. 1). It is reasonable to assume that COVID-19 causes a similar pathophysiology
to EBV-associated sHLH/MAS, as there are common abnormalities in both diseases, such
as hypercytokinemia, macrophage activation, cytopenias, immunological abnormalities
in CD8-positive cells, and the rapid development and progression of organ damage and
coagulopathy [1, 2, 5, 7, 9].
Fig. 1
Macrophage activation syndrome by COVID-19 and its treatment with low-dose etoposide
(author’s hypothesis). Low-dose etoposide is considered to restore immunological homeostasis
by depleting activated CTLs and suppressing their production of inflammatory cytokines,
which reduces the activity of macrophages and leads to the elimination of activated
macrophages and SARS-CoV-2-infected cells by newly activated CTLs
Etoposide is a chemotherapeutic drug widely used to treat various types of cancer,
including lymphoma, leukemia, and lung cancer, and promotes apoptosis of cancer cells
by inhibiting the topoisomerase II enzyme. Etoposide is also known to be effective
at low doses in combination with cyclosporine and steroids for familial HLH [10].
However, such combination therapy may be too immunosuppressive to eliminate virus-infected
cells in cases of virus-associated sHLH/MAS. Low-dose etoposide monotherapy, e.g.
a single dose of 100–150 mg/m2, 1–3 cycles, has been successfully used to treat sHLH/MAS
associated with EBV and autoimmune diseases, such as juvenile rheumatoid arthritis,
with a response rate of ≥ 80% reported [8, 11, 12]. Evidence supporting the usefulness
of etoposide treatment for sHLH/MAS can also be seen in a previous report [13], in
which low-dose etoposide substantially alleviated all symptoms of murine HLH and prolonged
the survival through the selective depletion of activated CTLs and suppression of
their inflammatory cytokine production. Importantly, low-dose etoposide essentially
spares quiescent naïve and memory T cells while ablating activated T cells [13, 14].
In another mouse model study [15], the combination of low-dose etoposide and prednisolone
improved the survival rate of fatal ARDS model mice with hypercytokinemia and hemophagocytosis,
which were induced by administration of α-galactosylceramide and lipopolysaccharide,
through suppressing the intrapulmonary recruitment and activation of macrophages,
T cells, NK cells, and neutrophils. Low-dose etoposide monotherapy also improved pulmonary
edema. Besides, the early introduction of low-dose etoposide was found to be effective
in patients with EBV-related sHLH/MAC with respiratory failure [16]. These findings
suggest that low-dose etoposide improves hypercytokinemia, renew CTLs so that activated
macrophages and SARS-CoV-2-infected cells are eliminated, and thus immunomodulatory
abnormalities associated with SARS-CoV-2 infection are restored, potentially improving
COVID-19 with ARDS (Fig. 1).
Like other chemotherapeutic agents, the major adverse effects of etoposide are associated
with off-target genotoxicity, dose-dependent myelosuppression and risks of secondary
cancer. However, treatment with low-dose etoposide for sHLH/MAS induced little hematologic
toxicity, instead of resulting in hematological improvement by restoring the bone
marrow function [8, 11, 12], and only 2 of over 600 patients who received low-dose
etoposide developed malignancies [10], supporting the use of low-dose etoposide for
the treatment of benign diseases. Moreover, the administration of up to five doses
of low-dose etoposide therapy to an adult costs approximately $80 (as of April 21,
2020), while a single dose of tocilizumab, a humanized monoclonal antibody against
the IL-6 receptor, costs $1000.
Given the above, the use of low-dose etoposide for severe COVID-19 may compensate
for the immunoregulatory aberration and macrophage activation causing the organ damage,
coagulopathy, and cytopenia, thereby leading to the restoration of homeostasis and
hopefully reduction in the mortality and morbidity rates. Given the high efficacy
and safety of low-dose etoposide for sHLH/MAS and the staggering mortality rate (as
high as 50%) associated with severe COVID-19, this monotherapy is worth considering
as a treatment for such patients.