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      Forming Model Optimization and Microstructural Analysis of Medical Biological Porous Scaffolds Fabricated by Selective Laser Melting (SLM)

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          Abstract

          Optimally designed bone implants with a suitable porous structure have similar mechanical properties to bone tissue and at the same time have good biocompatible and excellent bioactivity. The preparation of medical biological porous scaffolds by metal 3D printing technology is one of the most promising and attractive biomedical applications. The traditional regular porous and self-growing porous scaffolds were established by using CAD and C4D software, and the different scaffolds of three-dimensional models in similar porosity was obtained. A three-dimensional model of a scaffold with a porous structure was designed, and the porous scaffold was prepared by selective laser melting (SLM) technique, and its microstructure and mechanical properties were analysed. Under the similar porosity, the average hardness of the surface of the self-growing structure porous scaffold reached 236.5HV, and the stress at 5% compressive strain after heat treatment was close to 75 MPa. The original surface of the 316L porous scaffold made by SLM has the potential to effectively promote the differentiation of MG63 cells into osteoblasts. At the same time, the surface morphology and structure of the self-growth scaffold are similar to human cancellous bone, which is conducive to cell attachment and growth, so it is more suitable for repairing diseased parts of human bones.

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          Most cited references28

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          A study of the microstructural evolution during selective laser melting of Ti–6Al–4V

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            Processing and biocompatibility evaluation of laser processed porous titanium.

            The Laser Engineered Net Shaping (LENS) method was used to fabricate porous Ti implants. Porous Ti structures with controlled porosity in the range of 17-58 vol.% and pore size up to 800 microm were produced by controlling LENS parameters, which showed a broad range of mechanical strength of 24-463 MPa and a low Young's modulus of 2.6-44GPa. The effects of porous structure on bone cell responses were evaluated in vitro with human osteoblast cells (OPC1). The results showed that cells spread well on the surface of porous Ti and formed strong local adhesion. MTT assay indicated LENS processed porous Ti provides a preferential surface for bone cell proliferation. Porous Ti samples also stimulated faster OPC1 cell differentiation compared with polished Ti sheet, which could be due to the change in cell morphology within the pores of Ti samples. More extracellular matrix and a higher level of alkaline phosphatase expression were found on the porous samples than on the Ti sheet. This can be beneficial for faster integration of porous implant with host bone tissue. The results obtained also indicated that a critical pore size of 200 microm or higher is needed for cell ingrowth into the pores, below which OPC1 cells bridged the pore surface without any growth in the pores.
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              The effect of geometry on three-dimensional tissue growth

              Tissue formation is determined by uncountable biochemical signals between cells; in addition, physical parameters have been shown to exhibit significant effects on the level of the single cell. Beyond the cell, however, there is still no quantitative understanding of how geometry affects tissue growth, which is of much significance for bone healing and tissue engineering. In this paper, it is shown that the local growth rate of tissue formed by osteoblasts is strongly influenced by the geometrical features of channels in an artificial three-dimensional matrix. Curvature-driven effects and mechanical forces within the tissue may explain the growth patterns as demonstrated by numerical simulation and confocal laser scanning microscopy. This implies that cells within the tissue surface are able to sense and react to radii of curvature much larger than the size of the cells themselves. This has important implications towards the understanding of bone remodelling and defect healing as well as towards scaffold design in bone tissue engineering.
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                Author and article information

                Journal
                mr
                Materials Research
                Mat. Res.
                ABM, ABC, ABPol (São Carlos, , Brazil )
                1516-1439
                1980-5373
                2023
                : 26
                : e20230172
                Affiliations
                [01] Jinan orgnameShandong Jianzhu University orgdiv1School of Materials Science and Engineering China
                Article
                S1516-14392023000100283 S1516-1439(23)02600000283
                10.1590/1980-5373-mr-2023-0172
                b6a2c9f1-fc86-4a5c-a8ae-6203bfb7166b

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 19 March 2023
                : 05 June 2023
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 28, Pages: 0
                Product

                SciELO Brazil

                Categories
                Articles

                mechanical properties,Selective laser melting (SLM),porous biomaterial,self-growing porous scaffolds

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