Activity-based protein profiling: A graphical review

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Abstract

Activity-based protein profiling (ABPP) is a chemoproteomic technology that employs small chemical probes to directly interrogate protein function within complex proteomes. Since its initial application almost 25 years ago, ABPP has proven to be a powerful and versatile tool for addressing numerous challenges in drug discovery, including the development of highly selective small-molecule inhibitors, the discovery of new therapeutic targets, and the illumination of target proteins in tissues and organisms. This graphical review provides an overview of the rapid evolution of ABPP strategies, highlighting the versatility of the approach with selected examples of its successful application.

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Most cited references 34

Record: found
Abstract: not found
Article: not found

Click Chemistry: Diverse Chemical Function from a Few Good Reactions

Hartmuth Kolb, M G Finn, K. Barry Sharpless … (2001)

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Record: found
Abstract: found
Article: not found

Bioorthogonal chemistry: fishing for selectivity in a sea of functionality.

Ellen M. Sletten, Carolyn R. Bertozzi (2009)

The study of biomolecules in their native environments is a challenging task because of the vast complexity of cellular systems. Technologies developed in the last few years for the selective modification of biological species in living systems have yielded new insights into cellular processes. Key to these new techniques are bioorthogonal chemical reactions, whose components must react rapidly and selectively with each other under physiological conditions in the presence of the plethora of functionality necessary to sustain life. Herein we describe the bioorthogonal chemical reactions developed to date and how they can be used to study biomolecules.

0 comments Cited 307 times – based on 0 reviews      Review now

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Record: found
Abstract: found
Article: not found

Activity-based protein profiling: from enzyme chemistry to proteomic chemistry.

Benjamin Cravatt, Aaron T Wright, John Kozarich (2008)

Genome sequencing projects have provided researchers with a complete inventory of the predicted proteins produced by eukaryotic and prokaryotic organisms. Assignment of functions to these proteins represents one of the principal challenges for the field of proteomics. Activity-based protein profiling (ABPP) has emerged as a powerful chemical proteomic strategy to characterize enzyme function directly in native biological systems on a global scale. Here, we review the basic technology of ABPP, the enzyme classes addressable by this method, and the biological discoveries attributable to its application.

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Author and article information

Contributors

Exequiel O.J. Porta

Journal

Journal ID (nlm-ta): Curr Res Pharmacol Drug Discov

Journal ID (iso-abbrev): Curr Res Pharmacol Drug Discov

Title: Current Research in Pharmacology and Drug Discovery

Publisher: Elsevier

ISSN (Electronic): 2590-2571

Publication date PMC-release: 24 August 2023

Publication date Collection: 2023

Publication date (Electronic): 24 August 2023

Volume: 5

Electronic Location Identifier: 100164

Affiliations

[1]Department of Chemistry, Durham University, Durham, DH1 3LE, United Kingdom

Author notes

[∗ ]Corresponding author. exequiel.o.porta@ 123456durham.ac.uk

Article

Publisher Item ID: S2590-2571(23)00012-3 Publisher ID: 100164

DOI: 10.1016/j.crphar.2023.100164

PMC ID: 10484978

PubMed ID: 37692766

SO-VID: b5925311-152c-4b91-8d25-7a5005026e25

License:

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

History

Date received : 17 April 2023

Date revision received : 6 August 2023

Date accepted : 23 August 2023

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