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      Biomolecular analyses enable new insights into ancient Egyptian embalming

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          Abstract

          The ability of the ancient Egyptians to preserve the human body through embalming has not only fascinated people since antiquity, but also has always raised the question of how this outstanding chemical and ritual process was practically achieved. Here we integrate archaeological, philological and organic residue analyses, shedding new light on the practice and economy of embalming in ancient Egypt. We analysed the organic contents of 31 ceramic vessels recovered from a 26th Dynasty embalming workshop at Saqqara 1, 2 . These vessels were labelled according to their content and/or use, enabling us to correlate organic substances with their Egyptian names and specific embalming practices. We identified specific mixtures of fragrant or antiseptic oils, tars and resins that were used to embalm the head and treat the wrappings using gas chromatography–mass spectrometry analyses. Our study of the Saqqara workshop extends interpretations from a micro-level analysis highlighting the socio-economic status of a tomb owner 37 to macro-level interpretations of the society. The identification of non-local organic substances enables the reconstruction of trade networks that provided ancient Egyptian embalmers with the substances required for mummification. This extensive demand for foreign products promoted trade both within the Mediterranean 810 (for example, Pistacia and conifer by-products) and with tropical forest regions (for example, dammar and elemi). Additionally, we show that at Saqqara, antiu and sefet—well known from ancient texts and usually translated as ‘myrrh’ or ‘incense’ 1113 and ‘a sacred oil’ 13, 14 —refer to a coniferous oils-or-tars-based mixture and an unguent with plant additives, respectively.

          Abstract

          Philological analysis of labels and instructions, together with gas chromatography–mass spectrometry analysis of residues on vessels recovered from a 26th Dynasty embalming workshop at Saqqara, Egypt provide insights into ancient Egyptian embalming practices.

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          A generalization of the retention index system including linear temperature programmed gas—liquid partition chromatography

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            Chemical Alteration and Use of Beeswax Through Time: Accelerated Ageing Tests and Analysis of Archaeological Samples from Various Environmental Contexts

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              Gas chromatographic, mass spectrometric and stable carbon isotopic investigations of organic residues of plant oils and animal fats employed as illuminants in archaeological lamps from Egypt.

              Man's use of illuminants in lamps or as torches to extend the working day and range of environments accessible to him would have been a major technological advance in human civilisation. The most obvious evidence for this in the archaeological record comes from pottery and stone vessels showing sooting due to the use of a wick in conjunction with a lipid-based fuel or illuminant. A wide range of potential fuels would have been exploited depending upon availability and burning requirements. Reported herein are the results of chemical investigations of a number of lamps recovered from excavations of the site of Qasr Ibrim, Egypt. Gas chromatographic, mass spectrometric and stable carbon isotopic analyses of both free (solvent extractable) and 'bound'(released from solvent extracted pottery by base treatment) lipids have revealed a wide range of saturated fatty acids, hydroxy fatty acids and alpha, omega-dicarboxylic acids. Examination of the distributions of compounds and comparisons with the fatty acid compositions of modern plant oils have allowed a range of fats and oils to be recognised. Specific illuminants identified include Brassicaceae (Cruciferae) seed oil (most likely radish oil, Raphanus sativus), castor oil (from Ricinus communis), animal fat, with less diagnostic distributions and delta(13)C values being consistent with low stearic acid plant oils, such as linseed (Linum usitatissimum) or sesame (Sesamum indicum) oils. The identifications of the various oils and fats are supported by parallel investigations of illuminant residues produced by burning various oils in replica pottery lamps. The findings are entirely consistent with the classical writers including Strabo, Pliny and Theophrastrus.
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                Author and article information

                Contributors
                maxime.rageot@uni-tuebingen.de
                philipp.stockhammer@lmu.de
                Journal
                Nature
                Nature
                Nature
                Nature Publishing Group UK (London )
                0028-0836
                1476-4687
                1 February 2023
                1 February 2023
                2023
                : 614
                : 7947
                : 287-293
                Affiliations
                [1 ]GRID grid.5252.0, ISNI 0000 0004 1936 973X, Institute for Pre- and Protohistoric Archaeology and Archaeology of the Roman Provinces, , Ludwig Maximilian University of Munich, ; Munich, Germany
                [2 ]GRID grid.10392.39, ISNI 0000 0001 2190 1447, Department of Pre- and Protohistory, , Eberhard Karls University of Tübingen, ; Tübingen, Germany
                [3 ]GRID grid.10392.39, ISNI 0000 0001 2190 1447, Department of Egyptology, , Eberhard Karls University of Tübingen, ; Tübingen, Germany
                [4 ]GRID grid.8379.5, ISNI 0000 0001 1958 8658, Department of Egyptology, , Julius-Maximilians University, Würzburg, ; Würzburg, Germany
                [5 ]GRID grid.419725.c, ISNI 0000 0001 2151 8157, The Central Laboratories Network, , the National Research Centre, ; Cairo, Egypt
                [6 ]GRID grid.419725.c, ISNI 0000 0001 2151 8157, Packaging Materials Department, , the National Research Centre, ; Cairo, Egypt
                [7 ]Analytical Research Department, Robertet S.A., Grasse, France
                [8 ]GRID grid.5685.e, ISNI 0000 0004 1936 9668, BioArCh, , University of York, ; York, UK
                [9 ]GRID grid.7605.4, ISNI 0000 0001 2336 6580, Department of Life Sciences, , University of Turin, ; Turin, Italy
                [10 ]GRID grid.419518.0, ISNI 0000 0001 2159 1813, Max Planck Institute for Evolutionary Anthropology, ; Leipzig, Germany
                Author information
                http://orcid.org/0000-0002-2216-0676
                http://orcid.org/0000-0003-0623-5225
                http://orcid.org/0000-0002-1026-6975
                http://orcid.org/0000-0003-4702-9372
                Article
                5663
                10.1038/s41586-022-05663-4
                9908542
                36725928
                b4221580-bc4c-4cc2-a683-7085e3e3afdd
                © The Author(s) 2023

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 10 August 2022
                : 15 December 2022
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                © The Author(s), under exclusive licence to Springer Nature Limited 2023

                Uncategorized
                science in culture,lipids,environmental economics,bioanalytical chemistry,interdisciplinary studies

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