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      Presencia de nectomonadas metaciclicas de L. pifanoi en la hipofaringe de Lutzomyia youngi y la ingestion de azúcares Translated title: Metacyclical forms of L. pifanoi in the hypopharynx of Lutzomyia youngi and their sugar consumption

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          Abstract

          Se evidencia la presencia de promastigotos metacíclicos de Leishmania pifanoi en el conducto hipofaríngeo de Lutzomyia youngi infectados experimentalmente por ingurgitación sobre lesiones tarsales de hámsteres. La aparición de metacíclicos en la hipofaringe, cuya morfología se ilustra, ocurre entre los 5 y 9 días de desarrollo postprandial y es más frecuente en insectos alimentados con sacarosa comercial no refinada. Se investiga el papel de derivados aminados de glucosa y galactosa y también de aminoácidos, en la promoción y migración de los metacíclicos.

          Translated abstract

          The presence of metacyclical promastigotes of Leishmania pifanoi in the hypopharyngeal duct of Lutzomyia youngi is reported. The insects were experimentally infected by engorgement on the tarsal lesions of hamsters. The metacyclics, whose morphology is illustrated, appeared in the hypopharynx 5 to 9 days after engorgement; they were more frequently found in the insects fed on unrefined commercial sugar. They role of amino derivates of glucose and galactose, as well as of amino acids in the development and migration of metacyclics, is investigated.

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          Most cited references20

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          Leishmania donovani metacyclic promastigotes: transformation in vitro, lectin agglutination, complement resistance, and infectivity.

          Freshly transformed Leishmania donovani amastigotes from hamster spleen were used to establish axenic cultures at high density in a modified Grace's medium, which was only partly replenished when cultures were fed. Small, free-swimming, highly active stationary phase promastigotes with a short cell body and long flagellum were induced in this medium. The freshly transformed stationary phase promastigotes so induced were less able to bind peanut agglutinin, had more than 40-fold increased resistance to killing by normal human serum, and 15-fold increased infectivity both in vivo and in vitro when compared to freshly transformed logarithmic phase or long term culture promastigotes. These short form promastigotes may correspond to the metacyclic promastigote forms in the sand fly vector.
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            Leishmania Differentiation in Natural and Unnatural Sand Fly Hosts

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              Lectin binding strain-specific carbohydrates on the cell surfaces of Leishmania strains from the Old World.

              Four-day-old promastigote culture forms of L. tropica major from USSR (LV-252, LV-253, LRC L-38) and Saudi Arabia (Kö, Ha), L. tropica minor from USSR (LV-239, LRC L-39) Leishmania sp. from Israel (Ro) and Saudi Arabia (Ve-322, Schwe, Ne), L. donovani from Sudan (3S, 1-S, LV-139) and India (LV-125, LRC L-51), L donovani infantum from Israel (LV-140), and L. aethiopica from Ethiopia (LV-1, LV-16, LV-24, LV-26) were tested using the following lectins: C. ensiformis, R. communis-120, A. polypoides, P. vulgaris, E. europaeus, D. biflorus, L. tetragonolobus, U. europaeus, L. alpinum, A. papillata II, A. hypogaea, and S. hispida. All strains reacted with C. ensiformis, R. communis-120, and A. polypoides. No agglutination reactions were observed with P. vulgaris, D. biflorus, E. europaeus, and L. tetragonolobus. Agglutination differences were detected by reactions with A. papillata II, U. europaeus, L. alpinum, A. hypogaea, and S. hispida. L. tropica minor (LRC L-39, LV-249), L. donovani (LV-239, 1-S, 3S, LRC L-51), L. aethiopica (LV-1, LV-15, LV-24, LV-26), and L. tropica major (LB-242, LV-253, LRC LK-38, Kö, Ha) are distinguishable with lectins. From L. tropica major two intraspecific forms can be identified: USSR-type (LV-252, LV-253, LRC L-38) and a Near East-type (Kö, Ha). The Leishmania sp. strains (Ve-322, Ne, Ro, Schwe) belong to the Near East-type. The strains L. donovano LV-140 and L. donovani LV-125 react as L. tropica minor, a fact which cannot be elucidated. The L. donovani strains from Sudan cannot be distinguished from the Indian strain L. donovani LRC L-51.
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                Author and article information

                Journal
                rsp
                Revista de Saúde Pública
                Rev. Saúde Pública
                Faculdade de Saúde Pública da Universidade de São Paulo (São Paulo, SP, Brazil )
                0034-8910
                1518-8787
                June 1996
                : 30
                : 3
                : 240-247
                Affiliations
                [01] Trujillo orgnameCentro de Investigaciones Parasitológicas orgdiv1Control de Vectores orgdiv2Laboratorio de Quimioterapia Venezuela
                Article
                S0034-89101996000300006 S0034-8910(96)03000306
                b40b53f2-2d3a-42db-b5f6-9a0cc1c47bfb

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 05 January 1996
                : 20 March 1995
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 20, Pages: 8
                Product

                SciELO Brazil

                Self URI: Texto completo solamente en formato PDF (ES)

                Leishmania mexicana,Sucrose,Psychodidae,Sacaroso
                Leishmania mexicana, Sucrose, Psychodidae, Sacaroso

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