For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
29
views
131
references
 Top references
cited by
23
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      277
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      The Adenovirus Genome Contributes to the Structural Stability of the Virion

      review-article
      Author(s): 1 , 2 , 3 , 1 , 2 , 3 , 1 , 2 , 3 , 4 , *
      Publication date (Electronic):
      Journal: Viruses
      Publisher: MDPI
      Keywords: Adenovirus, virion stability, viral vectors, helper-dependent Ad

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Adenovirus (Ad) vectors are currently the most commonly used platform for therapeutic gene delivery in human gene therapy clinical trials. Although these vectors are effective, many researchers seek to further improve the safety and efficacy of Ad-based vectors through detailed characterization of basic Ad biology relevant to its function as a vector system. Most Ad vectors are deleted of key, or all, viral protein coding sequences, which functions to not only prevent virus replication but also increase the cloning capacity of the vector for foreign DNA. However, radical modifications to the genome size significantly decreases virion stability, suggesting that the virus genome plays a role in maintaining the physical stability of the Ad virion. Indeed, a similar relationship between genome size and virion stability has been noted for many viruses. This review discusses the impact of the genome size on Ad virion stability and emphasizes the need to consider this aspect of virus biology in Ad-based vector design.

          Related collections

          Most cited references131

          • Record: found
          • Abstract: found
          • Article: not found

          Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5.

          J M Bergelson, J. A. Cunningham, G Droguett … (1997)
          A complementary DNA clone has been isolated that encodes a coxsackievirus and adenovirus receptor (CAR). When transfected with CAR complementary DNA, nonpermissive hamster cells became susceptible to coxsackie B virus attachment and infection. Furthermore, consistent with previous studies demonstrating that adenovirus infection depends on attachment of a viral fiber to the target cell, CAR-transfected hamster cells bound adenovirus in a fiber-dependent fashion and showed a 100-fold increase in susceptibility to virus-mediated gene transfer. Identification of CAR as a receptor for these two unrelated and structurally distinct viral pathogens is important for understanding viral pathogenesis and has implications for therapeutic gene delivery with adenovirus vectors.
          Article 0 comments Cited 509 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Characteristics of a human cell line transformed by DNA from human adenovirus type 5.

            F Graham, J. Smiley, W Russell … (1977)
            Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in otheruman cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.
            Article 0 comments Cited 407 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.

              Louise Chow, Richard E. Gelinas, Thomas R. Broker … (1977)
              The 5' terminal sequences of several adenovirus 2 (Ad2) mRNAs, isolated late in infection, are complementary to sequences within the Ad2 genome which are remote from the DNA from which the main coding sequence of each mRNA is transcribed. This has been observed by forming RNA displacement loops (R loops) between Ad2 DNA and unfractionated polysomal RNA from infected cells. The 5' terminal sequences of mRNAs in R loops, variously located between positions 36 and 92, form complex secondary hybrids with single-stranded DNA from restriction endonuclease fragments containing sequences to the left of position 36 on the Ad2 genome. The structures visualized in the electron microscope show that short sequences coded at map positions 16.6, 19.6 and 26.6 on the R strand are joined to form a leader sequence of 150-200 nucleotides at the 5' end of many late mRNAs. A late mRNA which maps to the left of position 16.6 shows a different pattern of second site hybridization. It contains sequences from 4.9-6.0 linked directly to those from 9.6-10.9. These findings imply a new mechanism for the biosynthesis of Ad2 mRNA in mammalian cells.
              Article 0 comments Cited 209 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Viruses
                Journal ID (iso-abbrev): Viruses
                Journal ID (publisher-id): viruses
                Title: Viruses
                Publisher: MDPI
                ISSN (Electronic): 1999-4915
                Publication date (Electronic): 24 September 2014
                Publication date Collection: September 2014
                Volume: 6
                Issue: 9
                Pages: 3563-3583
                Affiliations
                [1 ]Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario K1H 8L6, Canada; E-Mails: bsaha@ 123456ohri.ca (B.S.); cawong@ 123456ohri.ca (C.M.W.)
                [2 ]Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
                [3 ]Centre for Neuromuscular Disease, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
                [4 ]Department of Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
                Author notes
                [* ]Author to whom correspondence should be addressed; E-Mail: rparks@ 123456ohri.ca ; Tel.: +1-613-737-8123, Fax: +1-613-737-8803.
                Article
                Publisher ID: viruses-06-03563
                DOI: 10.3390/v6093563
                PMC ID: 4189039
                PubMed ID: 25254384
                SO-VID: b22d0232-bc70-425f-a131-6ce32d4c48bc
                Copyright © © 2014 by the authors; licensee MDPI, Basel, Switzerland.
                License:

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).

                History
                Date received : 31 July 2014
                Date revision received : 15 September 2014
                Date accepted : 17 September 2014
                Categories
                Subject: Review

                ScienceOpen disciplines: Microbiology & Virology
                Keywords: adenovirus,virion stability,viral vectors,helper-dependent ad
                Data availability:
                ScienceOpen disciplines: Microbiology & Virology
                Keywords: adenovirus, virion stability, viral vectors, helper-dependent ad

                Comments

                Comment on this article

                scite_

                Similar content277

                See all similar

                Cited by23

                See all cited by

                Most referenced authors1,269

                See all reference authors