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      INFLUENCIA DE LA LISTERIOSIS EN LA FERTILIDAD Y PRESENTACIÓN DE MASTITIS SUBCLÍNICA EN UN CONGLOMERADO LECHERO DE LA SABANA DE BOGOTÁ, COLOMBIA Translated title: LISTERIOSIS INFLUENCE ON FERTILITY AND OCCURRENCE OF SUBCLINICAL MASTITIS IN A DAIRY CLUSTER OF SAVANNAH BOGOTA, COLOMBIA

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          Abstract

          Esta investigación tuvo como objetivo determinar la influencia de la listeriosis sobre el desempeño reproductivo y la presentación de mastitis subclínica, en un conglomerado lechero, de la Sabana de Bogotá. Se tomaron al azar ochenta y cuatro vacas en producción y en capacidad reproductiva, que fueron examinadas dos veces, con un intervalo de dos meses, para un total de 168 muestras, mediante la técnica de ELISA, para IFγ bovino específico para listeriosis. Los resultados indicaron una incidencia de 36% de positividad a la enfermedad. Las vacas positivas a IFγ presentaron mastitis subclínica grado 1 (P<0.00001) y grado 2 (P<0.3402); adicionalmente, las cifras de abortos en estas vacas fueron mayores (P<0.0002). En consecuencia, tuvieron mayor número de días abiertos (P<0.00001) y un número mayor de servicios por concepción (P<0.0093). Los resultados obtenidos ponen en evidencia los efectos negativos de la infección por Listeria monocytogenes sobre el tracto reproductivo y la responsabilidad del microorganismo en la presentación de mastitis en bovinos infectados.

          Translated abstract

          This research aimed to determine the influence of listeriosis on the reproductive performance and the presentation of mastitis in a dairy cluster of the Sabana de Bogotá. We randomly selected eighty four cows in production and reproductive capacity in four farms, which were examined twice with an interval of two months for a total of 168 samples by ELISA test for specific bovine IFγ for listeriosis. The results indicated an incidence of 36% of positivity to the disease. Positive cows for IFγ had grade 1 of subclinical mastitis (P <0.00001) and grade 2 (P <0.3402). The figures for abortions in these animals were higher than negatives (P <0.0002). Accordingly, had higher number of open days (P <0.00001) and a greater number of services per conception (P <0.0093). The results highlight the negative influence of Listeria monocytogenes infection on the reproductive tract and responsibility of the microorganism in the presentation of mastitis in infected cattle.

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              Current Issues on Molecular and Immunological Diagnosis of Tuberculosis

              Laboratory diagnosis of tuberculosis (TB) traditionally relies on smear microscopy and culture of Mycobacterium tuberculosis from clinical samples. With recent advances in technology, there have been numerous efforts to develop new diagnostic tests for TB that overcome the low sensitivity and specificity and long turnover time associated with current diagnostic tests. Molecular biological tests based on nucleic acid amplification have brought an unprecedented opportunity for the rapid and specific detection of M. tuberculosis from clinical specimens. With automated sequencing analysis, species identification of mycobacteria is now easier and more accurate than with conventional methods, and rapid detection of mutations in the genes associated with resistance to TB drugs provides early information on the potential drug resistance for each clinical isolate or for clinical samples. In addition, immunological tests for the detection of M. tuberculosis antigens and antibodies to the antigens have been explored to identify individuals at risk of developing TB or with latent TB infection (LTBI). The recent introduction of commercial IFN-γ assay kits for the detection of LTBI provides a new approach for TB control even in areas with a high incidence of TB. However, these molecular and immunological tools still require further evaluation using large scale cohort studies before implementation in TB control programs.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rudca
                Revista U.D.C.A Actualidad & Divulgación Científica
                rev.udcaactual.divulg.cient.
                Universidad de Ciencias Aplicadas y Ambientales
                0123-4226
                December 2015
                : 18
                : 2
                : 409-415
                Affiliations
                [1 ] Universidad de Ciencias Aplicadas y Ambientales U.D.C.A Colombia
                [2 ] Fundación FUNCEP Colombia
                [3 ] Particular Colombia
                [4 ] Universidad de Ciencias Aplicadas y Ambientales U.D.C.A Colombia
                Article
                S0123-42262015000200014
                b0f5ff20-e133-4df1-a30d-e4ca2f86b045

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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                SciELO Colombia

                Self URI (journal page): http://www.scielo.org.co/scielo.php?script=sci_serial&pid=0123-4226&lng=en
                Categories
                MULTIDISCIPLINARY SCIENCES

                Infertilidad,mastitis,ganado de leche,Infertility,dairy cattle

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