Contributors
Elizabeth P. Darga: Role: ConceptualizationRole: Data curationRole: Formal analysisRole: InvestigationRole:
MethodologyRole: Project administrationRole: ResourcesRole: ValidationRole: VisualizationRole:
Writing – original draftRole: Writing – review & editing
Emily M. Dolce:
ORCID: https://orcid.org/0000-0001-7212-8116
Role: Data curationRole: InvestigationRole: ResourcesRole: Writing – review & editing
Fang Fang: Role: Data curationRole: Formal analysisRole: MethodologyRole: SoftwareRole: Writing
– original draftRole: Writing – review & editing
Kelley M. Kidwell:
ORCID: https://orcid.org/0000-0002-1717-4483
Role: Data curationRole: Formal analysisRole: MethodologyRole: ResourcesRole: SoftwareRole:
SupervisionRole: ValidationRole: Writing – original draftRole: Writing – review &
editing
Christina L. Gersch: Role: Formal analysisRole: InvestigationRole: MethodologyRole: ResourcesRole: VisualizationRole:
Writing – review & editing
Steven Kregel: Role: InvestigationRole: MethodologyRole: ResourcesRole: Writing – review & editing
Dafydd G. Thomas: Role: InvestigationRole: ResourcesRole: Writing – review & editing
Anoop Gill: Role: ResourcesRole: Writing – review & editing
Martha E. Brown: Role: ResourcesRole: Writing – review & editing
Steven Gross: Role: ConceptualizationRole: InvestigationRole: Writing – review & editing
Mark Connelly: Role: ConceptualizationRole: InvestigationRole: Writing – review & editing
Michael Holinstat:
ORCID: https://orcid.org/0000-0001-5100-1933
Role: ConceptualizationRole: ResourcesRole: SupervisionRole: Writing – review & editing
Erin F. Cobain: Role: ConceptualizationRole: SupervisionRole: Writing – review & editing
James M. Rae: Role: ConceptualizationRole: ResourcesRole: SupervisionRole: Writing – review & editing
Daniel F. Hayes: Role: ConceptualizationRole: Funding acquisitionRole: Project administrationRole:
ResourcesRole: SupervisionRole: Writing – original draftRole: Writing – review & editing
Costanza Paoletti: Role: ConceptualizationRole: MethodologyRole: Project administrationRole: ResourcesRole:
SupervisionRole: Writing – original draftRole: Writing – review & editing
Dominique Heymann: Role: Editor
Journal
Journal ID (nlm-ta): PLoS One
Journal ID (iso-abbrev): PLoS One
Journal ID (publisher-id): plos
Title:
PLoS ONE
Publisher:
Public Library of Science
(San Francisco, CA USA
)
ISSN
(Electronic):
1932-6203
Publication date
(Electronic):
15
November
2021
Publication date Collection: 2021
Volume: 16
Issue: 11
Electronic Location Identifier: e0260124
Affiliations
[1
]
Breast Oncology Program, Department of Internal Medicine, University of Michigan Medical
School, Ann Arbor, Michigan, United States of America
[2
]
University of Michigan Rogel Cancer Center, University of Michigan, Ann Arbor, Michigan,
United States of America
[3
]
Department of Biostatistics, School of Public Health, University of Michigan, Ann
Arbor, Michigan, United States of America
[4
]
Michigan Center for Translational Pathology, University of Michigan, Ann Arbor, Michigan,
United States of America
[5
]
Menarini Silicon Biosystems, Huntingdon Valley, Pennsylvania, United States of America
[6
]
Departments of Pharmacology and Internal Medicine, University of Michigan Medical
School, Ann Arbor, Michigan, United States of America
Universite de Nantes, FRANCE
Author notes
Competing Interests: I have read the journal’s policy and the authors of this manuscript have the following
competing interests: C.P. reports non-financial support (travel paid), contracts,
and research support from Menarini Silicon Biosystems (MSB), the manufacturer of CellSearch®
and, outside the submitted work, research funding from AstraZeneca, and Pfizer. C.P.
is currently an employee of Eisai, Inc. University of Michigan (UM) has received funding
to support research on behalf of D.F.H. and from Menarini/Silicon Biosystems, manufacturer
of CellSearch. UM has submitted a patent application related to CTC and platelet PDL1
testing on which D.F.H., C.P., E.P.D. and E.M.D. are named inventors/co-inventors.UM
holds patent US 8,790,878 B2 for which D.F.H. is designated as inventor, and that
is licensed to MSB with annual royalties through January 2021. Outside the submitted
work D.F.H. holds stock options from InBiomotion, and serves on advisory boards for
Cepheid, Freenome, CellWorks, Lexent Bio, EPIC Science, Salutogenic Innovations, L-Nutra,
BioVeca, OncoCyte, Turnstone Biologics, Predictus BioSciences, Tempus, Guardant and
UM has received funding to support research on behalf of D.F.H. from Astra Zeneca,
Merrimack, Eli Lilly, Puma Biotechnology, Pfizer. Outside the submitted work M.H.
serves as a consultant and receives research support from Veralox Therapeutics. Outside
the submitted work E.F.C. has served advisory/consulting roles for AstraZeneca, Biotheranostics,
Ayala Pharmaceuticals and Athenex Oncology. The remaining co-authors have declared
that no competing interests exist.
[¤]
Current address: EISAI Inc., Woodcliff Lake, New Jersey, United States of America
Author information
Article
Publisher ID:
PONE-D-21-26910
DOI: 10.1371/journal.pone.0260124
PMC ID: 8592410
PubMed ID: 34780566
SO-VID: afe7ebb1-0270-4498-b633-be577f8591f4
Copyright © © 2021 Darga et al
License:
This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
History
Date
received
: 19
August
2021
Date
accepted
: 2
November
2021
Page count
Figures: 4,
Tables: 4,
Pages: 19
Funding
Funded by:
Menarini Silicon Biosystems
Award Recipient
:
Daniel F. Hayes
Funded by:
the Fashion Footwear Charitable Foundation of New York/QVC Presents Shoes on Sale
™
Award Recipient
:
Daniel F. Hayes
Funded by:
National Cancer Institute Cancer Center Support Grant
Award ID: P30CA046592
This work was supported by Menarini Silicon Biosystems, the Fashion Footwear Charitable
Foundation of New York/QVC Presents Shoes on Sale ™ (D.F.H.), and the National Cancer
Institute Cancer Center Support Grant (P30CA046592). The funders had no role in study
design, data collection and analysis, decision to publish, or preparation of the manuscript.
Categories
Subject:
Research Article
Subject:
Biology and Life Sciences
Subject:
Anatomy
Subject:
Body Fluids
Subject:
Blood
Subject:
Platelets
Subject:
Medicine and Health Sciences
Subject:
Anatomy
Subject:
Body Fluids
Subject:
Blood
Subject:
Platelets
Subject:
Biology and Life Sciences
Subject:
Physiology
Subject:
Body Fluids
Subject:
Blood
Subject:
Platelets
Subject:
Biology and Life Sciences
Subject:
Cell Biology
Subject:
Cellular Types
Subject:
Animal Cells
Subject:
Blood Cells
Subject:
Platelets
Subject:
Research and Analysis Methods
Subject:
Specimen Preparation and Treatment
Subject:
Staining
Subject:
Cell Staining
Subject:
Medicine and Health Sciences
Subject:
Oncology
Subject:
Cancers and Neoplasms
Subject:
Breast Tumors
Subject:
Breast Cancer
Subject:
Biology and Life Sciences
Subject:
Anatomy
Subject:
Body Fluids
Subject:
Blood
Subject:
Medicine and Health Sciences
Subject:
Anatomy
Subject:
Body Fluids
Subject:
Blood
Subject:
Biology and Life Sciences
Subject:
Physiology
Subject:
Body Fluids
Subject:
Blood
Subject:
Medicine and Health Sciences
Subject:
Oncology
Subject:
Cancer Treatment
Subject:
Medicine and Health Sciences
Subject:
Oncology
Subject:
Metastasis
Subject:
Medicine and Health Sciences
Subject:
Oncology
Subject:
Basic Cancer Research
Subject:
Metastasis
Subject:
Biology and life sciences
Subject:
Genetics
Subject:
Gene expression
Subject:
Gene regulation
Subject:
Small interfering RNA
Subject:
Biology and life sciences
Subject:
Biochemistry
Subject:
Nucleic acids
Subject:
RNA
Subject:
Non-coding RNA
Subject:
Small interfering RNA
Subject:
Research and analysis methods
Subject:
Specimen preparation and treatment
Subject:
Staining
Subject:
Nuclear staining
Subject:
DAPI staining
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