Kinesin 1 Drives Autolysosome Tubulation.

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Abstract

Autophagic lysosome reformation (ALR) plays an important role in maintaining lysosome homeostasis. During ALR, lysosomes are reformed by recycling lysosomal components from autolysosomes. The most noticeable step of ALR is autolysosome tubulation, but it is currently unknown how the process is regulated. Here, using an approach combining in vivo studies and in vitro reconstitution, we found that the kinesin motor protein KIF5B is required for autolysosome tubulation and that KIF5B drives autolysosome tubulation by pulling on the autolysosomal membrane. Furthermore, we show that KIF5B directly interacts with PtdIns(4,5)P2. Kinesin motors are recruited and clustered on autolysosomes via interaction with PtdIns(4,5)P2 in a clathrin-dependent manner. Finally, we demonstrate that clathrin promotes formation of PtdIns(4,5)P2-enriched microdomains, which are required for clustering of KIF5B. Our study reveals a mechanism by which autolysosome tubulation was generated.

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Journal

Journal ID (iso-abbrev): Dev. Cell

Title: Developmental cell

Publisher: Elsevier BV

ISSN (Electronic): 1878-1551

ISSN (Print): 1534-5807

Publication date (Electronic): May 23 2016

Volume: 37

Issue: 4

Affiliations

[1 ] State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China; School of Life Sciences, Tsinghua University, Beijing 100084, China.

[2 ] State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Peking University, Beijing 100871, China.

[3 ] State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.

[4 ] School of Life Sciences, Peking University, Beijing 100871, China.

[5 ] School of Life Sciences, Tsinghua University, Beijing 100084, China.

[6 ] State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Peking University, Beijing 100871, China; School of Life Sciences, Peking University, Beijing 100871, China. Electronic address: sun_yujie@pku.edu.cn.

[7 ] State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address: liyulab@mail.tsinghua.edu.cn.

Article

Publisher Item ID: S1534-5807(16)30234-9

DOI: 10.1016/j.devcel.2016.04.014

PubMed ID: 27219061

SO-VID: af3b9d01-d8f2-4b56-aa0c-af77301056d6

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