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      Estudio comparativo de la Digestibilidad de forrajes mediante dos Métodos de Laboratorio

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          Abstract

          El objetivo de este estudio fue estandarizar un método enzimático para medir la digestibilidad de forrajes empleando la enzima celulasa proveniente del hongo Penicillium funiculosum y compararlo con el método de digestibilidad in vitro de Tilley y Terry (1963). Para la estandarización de la técnica se utilizó como sustrato forrajes de digestibilidad in vitro conocida: alfalfa y paja de avena; y para la comparación entre métodos se utilizó forrajes de diferente calidad provenientes de varias zonas del Perú. La concentración óptima enzima-sustrato hallada fue de 200 y 250 mg, respectivamente. Los valores de digestibilidad del método enzimático fueron estadísticamente menores que los del método in vitro, y el coeficiente de correlación fue de 0.987 (p<0.001). El método de digestibilidad enzimática demostró ser un procedimiento de laboratorio simple y rápido para la evaluación de los forrajes.

          Translated abstract

          The objective of this study was to standardize the enzymatic procedure for estimating the digestibility of forages using the fungal cellulase Penicillium funiculosum, and its comparison with the Tilley and Terry (1963) in vitro digestibility method. The well- known in vitro digestibility of alfalfa and oat straw were used for the standardization of the technique. Different quality forages from various locations of Peru were used for the comparison between methods. The best enzyme-substrate concentrations were 200 and 250 mg respectively. Both methods were statistically different, being the enzymatic digestibility values lower than the in vitro method ones. The correlation coefficient between methods was 0.987 (p<0.001). The enzymatic digestibility method proved to be a simple and rapid laboratory analysis for the evaluation of forages.

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          The effect of pepsin pretreatment of herbage on the prediction of dry matter digestibility from solubility in fungal cellulase solutions

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            A two stage technique for in vitro digestion of forage crops

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              Enzymatic Evaluation of Processes for Improving Agricultural Wastes for Ruminant Feeds

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                Author and article information

                Journal
                rivep
                Revista de Investigaciones Veterinarias del Perú
                Rev. investig. vet. Perú
                Universidad Nacional Mayor de San Marcos. Facultad de Medicina Veterinaria (Lima, , Peru )
                1609-9117
                January 2003
                : 14
                : 1
                : 07-12
                Affiliations
                [02] orgnameUNMSM orgdiv1FMV orgdiv2Laboratorio de Bioquímica, Nutrición y Alimentación Animal
                Article
                S1609-91172003000100002 S1609-9117(03)01400102
                aeb8ad5c-ac2a-41c6-bc98-1344ecec88a8

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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                Figures: 0, Tables: 0, Equations: 0, References: 13, Pages: 6
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                SciELO Peru

                Self URI: Texto completo solamente en formato PDF (ES)
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                ARTÍCULOS PRIMARIOS

                cellulase,digestibilidad,forraje,celulasa,digestibility,forage
                cellulase, digestibilidad, forraje, celulasa, digestibility, forage

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