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      Update on Streptococcus suis Research and Prevention in the Era of Antimicrobial Restriction: 4th International Workshop on S. suis

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          Abstract

          Streptococcus suis is a swine pathogen and a zoonotic agent afflicting people in close contact with infected pigs or pork meat. Sporadic cases of human infections have been reported worldwide. In addition, S. suis outbreaks emerged in Asia, making this bacterium a primary health concern in this part of the globe. In pigs, S. suis disease results in decreased performance and increased mortality, which have a significant economic impact on swine production worldwide. Facing the new regulations in preventive use of antimicrobials in livestock and lack of effective vaccines, control of S. suis infections is worrisome. Increasing and sharing of knowledge on this pathogen is of utmost importance. As such, the pathogenesis and epidemiology of the infection, antimicrobial resistance, progress on diagnosis, prevention, and control were among the topics discussed during the 4th International Workshop on Streptococcus suis (held in Montreal, Canada, June 2019). This review gathers together recent findings on this important pathogen from lectures performed by lead researchers from several countries including Australia, Canada, France, Germany, Japan, Spain, Thailand, The Netherlands, UK, and USA. Finally, policies and recommendations for the manufacture, quality control, and use of inactivated autogenous vaccines are addressed to advance this important field in veterinary medicine.

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          Microbiota-mediated colonization resistance against intestinal pathogens.

          Commensal bacteria inhabit mucosal and epidermal surfaces in mice and humans, and have effects on metabolic and immune pathways in their hosts. Recent studies indicate that the commensal microbiota can be manipulated to prevent and even to cure infections that are caused by pathogenic bacteria, particularly pathogens that are broadly resistant to antibiotics, such as vancomycin-resistant Enterococcus faecium, Gram-negative Enterobacteriaceae and Clostridium difficile. In this Review, we discuss how immune- mediated colonization resistance against antibiotic-resistant intestinal pathogens is influenced by the composition of the commensal microbiota. We also review recent advances characterizing the ability of different commensal bacterial families, genera and species to restore colonization resistance to intestinal pathogens in antibiotic-treated hosts.
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            Streptococcus suis, an important pig pathogen and emerging zoonotic agent—an update on the worldwide distribution based on serotyping and sequence typing

            Streptococcus suis is an important pathogen causing economic problems in the pig industry. Moreover, it is a zoonotic agent causing severe infections to people in close contact with infected pigs or pork-derived products. Although considered sporadic in the past, human S. suis infections have been reported during the last 45 years, with two large outbreaks recorded in China. In fact, the number of reported human cases has significantly increased in recent years. In this review, we present the worldwide distribution of serotypes and sequence types (STs), as determined by multilocus sequence typing, for pigs (between 2002 and 2013) and humans (between 1968 and 2013). The methods employed for S. suis identification and typing, the current epidemiological knowledge regarding serotypes and STs and the zoonotic potential of S. suis are discussed. Increased awareness of S. suis in both human and veterinary diagnostic laboratories and further establishment of typing methods will contribute to our knowledge of this pathogen, especially in regions where complete and/or recent data is lacking. More research is required to understand differences in virulence that occur among S. suis strains and if these differences can be associated with specific serotypes or STs.
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              Development of an enhanced human gastrointestinal epithelial culture system to facilitate patient-based assays.

              The technology for the growth of human intestinal epithelial cells is rapidly progressing. An exciting possibility is that this system could serve as a platform for individualised medicine and research. However, to achieve this goal, human epithelial culture must be enhanced so that biopsies from individuals can be used to reproducibly generate cell lines in a short time frame so that multiple, functional assays can be performed (ie, barrier function and host-microbial interactions).
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                Author and article information

                Journal
                Pathogens
                Pathogens
                pathogens
                Pathogens
                MDPI
                2076-0817
                14 May 2020
                May 2020
                : 9
                : 5
                : 374
                Affiliations
                [1 ]Research Group on Infectious Diseases in Production Animals and Swine and Poultry Infectious Diseases Research Centre, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, QC J2S 2M2, Canada
                [2 ]IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain; virginia.aragon@ 123456irta.es
                [3 ]USDA, ARS, National Animal Disease Center, Ames, IA 50010, USA; susan.brockmeier@ 123456usda.gov
                [4 ]College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA; gebha001@ 123456umn.edu
                [5 ]Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands; astrid.degreeff@ 123456wur.nl
                [6 ]Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand; Anusak.ke@ 123456ku.th
                [7 ]Antimicrobial Resistance and Infectious Disease Laboratory, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia 6150, Australia; m.odea@ 123456murdoch.edu.au
                [8 ]Division of Bacterial and Parasitic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan; mokura@ 123456affrc.go.jp
                [9 ]French Agency for Veterinary Medicinal Products-French Agency for food, Environmental and Occupational Health Safety (Anses-ANMV), 35302 Fougères, France; mariette.salery@ 123456anses.fr
                [10 ]Department of Global Health-Amsterdam Institute for Global Health and Development and Department of Medical Microbiology, Amsterdam University Medical Centers, University of Amsterdam, 1105 BP Amsterdam, The Netherlands; c.schultsz@ 123456aighd.org
                [11 ]Institute for Microbiology, University of Veterinary Medicine, 30173 Hannover, Germany; Peter.Valentin@ 123456tiho-hannover.de
                [12 ]Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK; lw461@ 123456cam.ac.uk
                [13 ]Host-Microbe Interactomics Group, Department Animal Sciences, Wageningen University and Research, 6709 PG Wageningen, The Netherlands; jerry.wells@ 123456wur.nl
                [14 ]Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK
                Author notes
                [* ]Correspondence: mariela.segura@ 123456umontreal.ca (M.S.); marcelo.gottschalk@ 123456umontreal.ca (M.G.); Tel.: +1-450-773-8521 (ext. 0080) (M.S.); +1-450-773-8521 (ext. 8374) (M.G.)
                [†]

                The 4th International Workshop on Streptococcus suis was sponsored by the Organisation for Economic Co-operation and Development (OECD), Co-operative Research Programme on Biological Resource Management for Sustainable Agricultural Systems, whose financial support made it possible for most of the invited speakers to participate in the workshop.

                The opinions expressed and arguments employed in this publication are the sole responsibility of the authors and do not necessarily reflect those of the OECD or of the governments of its Member countries.

                [‡]

                Co-authors are listed in alphabetical order.

                Author information
                https://orcid.org/0000-0002-3470-6015
                https://orcid.org/0000-0002-7066-9178
                https://orcid.org/0000-0003-1055-3656
                https://orcid.org/0000-0002-2757-7585
                Article
                pathogens-09-00374
                10.3390/pathogens9050374
                7281350
                32422856
                ae96e928-14c9-40c2-9879-7c60a336ea2a
                © 2020 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 19 April 2020
                : 08 May 2020
                Categories
                Conference Report

                streptococcus suis,swine,zoonosis,epidemiology,genomics,diagnosis,antimicrobials,vaccines,public health,vaccine policies

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