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Abstract
A three-stage anaerobic fermentation process including H(2) fermentation I, H(2) fermentation
II, methane fermentation was developed for the coproduction of hydrogen and methane
from cornstalks. Hydrogen production from cornstalks using direct microbial conversion
by Clostridium thermocellum 7072 was markedly enhanced in the two-stage thermophilic
hydrogen fermentation process integrated with alkaline treatment. The highest total
hydrogen yield from cornstalks in the two-stage fermentation process reached 74.4
mL/g-cornstalk. The hydrogen fermentation effluents and alkaline hydrolyzate were
further used for methane fermentation by anaerobic granular sludge, and the total
methane yield reached 205.8 mL/g-cornstalk. The total energy recovery in the three-stage
anaerobic fermentation process integrated with alkaline hydrolysis reached 70.0%.