Resistance to Bacillus thuringiensis Cry1Ac toxin requires mutations in two Plutella xylostella ATP-binding cassette transporter paralogs

Abstract

The diamondback moth, Plutella xylostella, is a cosmopolitan pest and the first species to develop field resistance to toxins from the gram-positive bacterium Bacillus thuringiensis (Bt). Although previous work has suggested that mutations of ATP-binding cassette transporter subfamily C2 (ABCC2) or C3 (ABCC3) genes can confer Cry1Ac resistance, here we reveal that P. xylostella requires combined mutations in both PxABCC2 and PxABCC3 to achieve high-level Cry1Ac resistance, rather than simply a mutation of either gene. We identified natural mutations of PxABCC2 and PxABCC3 that concurrently occurred in a Cry1Ac-resistant strain (Cry1S1000) of P. xylostella, with a mutation ( R _A2 ) causing the mis-splicing of PxABCC2 and another mutation ( R _A3 ) leading to the premature termination of PxABCC3. Genetic linkage analysis showed that R _A2 and R _A3 were tightly linked to Cry1Ac resistance. Introgression of R _A2 and R _A3 enabled a susceptible strain (G88) of P. xylostella to obtain high resistance to Cry1Ac, confirming that these genes confer resistance. To further support the role of PxABCC2 and PxABCC3 in Cry1Ac resistance, frameshift mutations were introduced into PxABCC2 and PxABCC3 singly and in combination in the G88 strain with CRISPR/Cas9 mediated mutagenesis. Bioassays of CRISPR-based mutant strains, plus genetic complementation tests, demonstrated that the deletion of PxABCC2 or PxABCC3 alone provided < 4-fold tolerance to Cry1Ac, while disruption of both genes together conferred >8,000-fold resistance to Cry1Ac, suggesting the redundant/complementary roles of PxABCC2 and PxABCC3. This work advances our understanding of Bt resistance in P. xylostella by demonstrating mutations within both PxABCC2 and PxABCC3 genes are required for high-level Cry1Ac resistance.

Author summary

Bacillus thuringiensis (Bt) foliar sprays and transgenic crops expressing Bt toxins are used extensively to control insect pests, but the evolution of resistance limits their efficacy. Multiple studies have reported that ATP-binding cassette (ABC) transporters are important Bt receptors, and mutations in either ABCC2 or ABCC3 can lead to Cry1Ac-toxin resistance, although this process is not fully understood. In this study, we applied both forward and reverse genetic analyses to demonstrate that high-level Bt-Cry1Ac resistance in Plutella xylostella requires concurrent mutations in both PxABCC2 and PxABCC3. We identified inactivating mutations in these two genes from a Cry1Ac-resistant strain (Cry1S1000) of P. xylostella and conducted genetic linkage analysis, which supported the role that PxABCC2 and PxABCC3 were the causal genes of Cry1Ac resistance. We then knocked out PxABCC2 and PxABCC3 in a P. xylostella susceptible reference strain (G88) to confirm that high-level Cry1Ac resistance requires mutation of PxABCC2 and PxABCC3, rather than a mutation of either one gene. This finding expands our understanding of complex Bt resistance processes and may be relevant to Bt-Cry1Ac resistance in other lepidopteran insects.