A pilot and comparative study between pathological and serological levels of immunoglobulin and complement among three kinds of primary glomerulonephritis

Abstract

Background

Immunoglobulin A nephropathy (IgAN), membranous nephropathy (MN) and minimal-change disease (MCD) are three common types of glomerulonephritis in China. Pathological diagnosis based on renal biopsy is the criterion and the golden standard for diagnosing the sub-types of primary or secondary glomerulonephritis. Immunoglobulin and complements might be used in the differential diagnosis of glomerulonephritis without renal biopsies. However, the relationship between IF intensities of immune proteins and the corresponding serum levels remained unclear, and seldom studies combine histopathological examination results and blood tests together for a predictive purpose. This study was considered as a pilot study for integrating histopathological indicators into serum parameters for exploring the relationship of IF intensity and serum values of immunoglobulin and complement, and for screening and investigating effective indicators inIgAN, MN and MCD.

Methods

Renal tissue immunofluorescence (IF) intensity grades and serum levels of immunoglobulin and complements (IgG, IgA, IgM, C3 and C4) were retrospectively analyzed in 236 cases with IgAN, MN or MCD. IF grades were grouped as negative (−), positive (+) or strong positive (++) with both high and low magnification of microscope. Other serum indicators such as urea nitrogen (BUN), creatinine (Crea) and estimated glomerular filtration rate (eGFR) were also evaluated among the groups.

Results

There were difference in IgA, IgG and C3 IF intensity grades among IgAN, MN and MCD groups ( p = 9.82E-43, 4.60E-39, 7.45E-15, respectively). Serum values of BUN, Crea, eGFR, IgG, IgA, IgM and C4 showed difference in three groups (BUN: p = 0.045, Crea: p = 3.45E-5, eGFR: p = 0.005, IgG: p = 1.68E-14, IgA: p = 9.14E-9, IgM: p = 0.014, C4: p = 0.026). eGFR had the trend to decrease with enhanced IgA IF positive grades ( p = 8.99E-4); Crea had trends to decrease with both enhanced IgA and IgG IF intensity grades ( p = 2.06E-6, 2.94E-5, respectively). In all subjects, serum IgA levels was inversely correlated with eGFR( r = − 0.216, p = 0.001) and correlated with Crea levels( r = 0.189, p = 0.004); serum IgG and Crea showed no correlation which were discordance with inverse correlation of IgG IF grade and Crea( r = 0.058, p = 0.379). IgG serum level was inverse correlated with its IF grades ( p = 3.54E-5, p = 7.08E-6, respectively); C3 serum levels had significantly difference between Neg and positive (+) group ( p = 0.0003). IgA serum level was positive correlated with its IF grades (Neg-(+): p = 0.0001; (+)-(++): p = 0.022; Neg-(++): p = 2.01E-10). After matching comparison among C3 groups, C3 Neg. group and C3 ++ group had difference (* p = 0.017). C4 had all negative IF expression in all pathological groups. In IgAN subjects, there were statistical differences of serum C3 levels between its pathological Neg and positive (+) group( p = 0.026), and serum IgA levels showed difference between IgA pathological positive(+) and (++)( p = 0.007). In MN subjects, sIgG levels showed difference between IgG pathological IF grade positive (+) and (++)( p = 0.044); serum C3 levels showed difference between C3 pathological IF grade Neg and positive(+)( p = 0.005); and serum IgA levels showed difference between Neg and positive(+)( p = 0.040). In IgAN, eGFR showed serum IgA levels had significant differences among groups ( p = 0.007) and had increasing trend with enhanced its IF grades(P _trend = 0.016). There were also difference between IgG group Neg and positive (+) ( p = 0.005, P _trend = 0.007) in IgAN. In MN, serum IgG levels had significant differences among IF groups ( p = 0.034) and had decreasing trend with its enhanced IF grades (P _trend = 0.014). Serum C3 concentrations also were found distinctive among IF groups ( p = 0.016) and had in inverse correlation with its enhanced IF grades (P _trend = 0.004).

Discussion

Our research cross contrasts several immunoprotein IF intensities and relevant serum levels in three kinds of primary glomerular nephritis, and finally acquired helpful results for understanding the relationships between pathological presentation and serological presentation of immunoproteins in kidney diseases. Furthermore, this pilot study is offering a possible method for the analysis of combination of pathology and serology.

Conclusion

Different pathological types of nephritis presented different expression patterns of immunoglobulin and complement, especially IgA and IgG, which suggested different pathogenesis involved in the development of IgAN and MN. Furthermore, either in tissue or in serum, increased IgA level was closely related with renal function in all of the patients.

Electronic supplementary material

The online version of this article (10.1186/s12865-018-0254-z) contains supplementary material, which is available to authorized users.