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      Phyllocnistis furcata sp. nov.: a new species of leaf-miner associated with Baccharis (Asteraceae) from Southern Peru (Lepidoptera, Gracillariidae)

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          Abstract

          The southwestern Andes of Peru harbors a hidden taxonomic diversity of Lepidoptera . Here a new leaf-mining species of Gracillariidae ( Lepidoptera ) is described, Phyllocnistis furcata Vargas & Cerdeña, sp. nov., from a dry Andean valley of southern Peru, at 2400 m above sea level. The morphological aspects of adults (male and female) and the immature stages associated with Baccharis alnifolia Meyen & Walp. ( Asteraceae ) are given, under optical microscopy and scanning electron microscopy. DNA barcodes show that its nearest neighbor is the Atlantic Forest species Phyllocnistis ourea Brito & Moreira, 2017 that feeds on Baccharis anomala DC. The importance of morphological characters from immature stages for diagnosis among congeneric species is also discussed. Phyllocnistis furcata represents the fourth species of Phyllocnistis Zeller for Peru, and first record from the south of Peru for the genus.

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          MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.

          The Molecular Evolutionary Genetics Analysis (Mega) software implements many analytical methods and tools for phylogenomics and phylomedicine. Here, we report a transformation of Mega to enable cross-platform use on Microsoft Windows and Linux operating systems. Mega X does not require virtualization or emulation software and provides a uniform user experience across platforms. Mega X has additionally been upgraded to use multiple computing cores for many molecular evolutionary analyses. Mega X is available in two interfaces (graphical and command line) and can be downloaded from www.megasoftware.net free of charge.
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            New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0.

            PhyML is a phylogeny software based on the maximum-likelihood principle. Early PhyML versions used a fast algorithm performing nearest neighbor interchanges to improve a reasonable starting tree topology. Since the original publication (Guindon S., Gascuel O. 2003. A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 52:696-704), PhyML has been widely used (>2500 citations in ISI Web of Science) because of its simplicity and a fair compromise between accuracy and speed. In the meantime, research around PhyML has continued, and this article describes the new algorithms and methods implemented in the program. First, we introduce a new algorithm to search the tree space with user-defined intensity using subtree pruning and regrafting topological moves. The parsimony criterion is used here to filter out the least promising topology modifications with respect to the likelihood function. The analysis of a large collection of real nucleotide and amino acid data sets of various sizes demonstrates the good performance of this method. Second, we describe a new test to assess the support of the data for internal branches of a phylogeny. This approach extends the recently proposed approximate likelihood-ratio test and relies on a nonparametric, Shimodaira-Hasegawa-like procedure. A detailed analysis of real alignments sheds light on the links between this new approach and the more classical nonparametric bootstrap method. Overall, our tests show that the last version (3.0) of PhyML is fast, accurate, stable, and ready to use. A Web server and binary files are available from http://www.atgc-montpellier.fr/phyml/.
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              Biodiversity hotspots for conservation priorities.

              Conservationists are far from able to assist all species under threat, if only for lack of funding. This places a premium on priorities: how can we support the most species at the least cost? One way is to identify 'biodiversity hotspots' where exceptional concentrations of endemic species are undergoing exceptional loss of habitat. As many as 44% of all species of vascular plants and 35% of all species in four vertebrate groups are confined to 25 hotspots comprising only 1.4% of the land surface of the Earth. This opens the way for a 'silver bullet' strategy on the part of conservation planners, focusing on these hotspots in proportion to their share of the world's species at risk.
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                Author and article information

                Contributors
                Journal
                Zookeys
                Zookeys
                2
                urn:lsid:arphahub.com:pub:45048D35-BB1D-5CE8-9668-537E44BD4C7E
                urn:lsid:zoobank.org:pub:91BD42D4-90F1-4B45-9350-EEF175B1727A
                ZooKeys
                Pensoft Publishers
                1313-2989
                1313-2970
                2020
                24 November 2020
                : 996
                : 121-145
                Affiliations
                [1 ] PPG Biologia Animal, Departamento de Zoologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Porto Alegre, RS 91501-970, Brazil Universidad Nacional de San Agustín de Arequipa Arequipa Peru
                [2 ] Universidad Nacional de San Agustín de Arequipa, Museo de Historia Natural, Av. Alcides Carrión s/n, Arequipa, Peru Universidade Federal do Rio Grande do Sul Porto Alegre Brazil
                [3 ] Departamento de Recursos Ambientales, Facultad de Ciencias Agronómicas, Universidad de Tarapacá, Casilla 6-D, Arica, Chile Universidad de Tarapacá Arica Chile
                [4 ] Embrapa Cerrados, Planaltina, Distrito Federal, Brazil Universidad Nacional de San Agustín de Arequipa Arequipa Peru
                [5 ] Departamento de Zoologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, Porto Alegre RS, 91501-970, Brazil Universidade Federal do Rio Grande do Sul Porto Alegre Brazil
                [6 ] Universidad Nacional de San Agustín de Arequipa, Laboratorio Fisiologia Animal, Av. Alcides Carrión s/n, Arequipa, Peru Universidad de Tarapacá Arica Chile
                Author notes
                Corresponding author: José Cerdeña ( cerdenajoseal@ 123456yahoo.es )

                Academic editor: E. van Nieukerken

                Author information
                https://orcid.org/0000-0003-3930-6480
                https://orcid.org/0000-0002-5355-3157
                https://orcid.org/0000-0003-3132-7164
                Article
                53958
                10.3897/zookeys.996.53958
                7710689
                ac0d3037-6315-4bb2-b7a7-e4a0d6ad92fe
                José Cerdeña, Jackie Farfán, Héctor A. Vargas, Rosângela Brito, Gislene L. Gonçalves, Ana Lazo, Gilson R. P. Moreira

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 06 May 2020
                : 24 August 2020
                Categories
                Research Article
                Animalia
                Arthropoda
                Gracillariidae
                Hexapoda
                Insecta
                Invertebrata
                Lepidoptera
                Systematics
                Taxonomy
                Americas
                Peru
                South America

                Animal science & Zoology
                andes,arequipa,barcoding,immature stages, phyllocnistinae
                Animal science & Zoology
                andes, arequipa, barcoding, immature stages, phyllocnistinae

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