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      Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water Translated title: Parâmetros bioquímicos do jundiá (Rhamdia quelen) após transporte com eugenol ou óleo essencial de Lippia alba adicionado à água

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          Abstract

          Abstract The transport of live fish is a routine practice in aquaculture and constitutes a considerable source of stress to the animals. The addition of anesthetic to the water used for fish transport can prevent or mitigate the deleterious effects of transport stress. This study investigated the effects of the addition of eugenol (EUG) (1.5 or 3.0 µL L–1) and essential oil of Lippia alba (EOL) (10 or 20 µL L–1) on metabolic parameters (glycogen, lactate and total protein levels) in liver and muscle, acetylcholinesterase activity (AChE) in muscle and brain, and the levels of protein carbonyl (PC), thiobarbituric acid reactive substances (TBARS) and nonprotein thiol groups (NPSH) and activity of glutathione-S-transferase in the liver of silver catfish (Rhamdia quelen; Quoy and Gaimard, 1824) transported for four hours in plastic bags (loading density of 169.2 g L–1). The addition of various concentrations of EUG (1.5 or 3.0 µL L–1) and EOL (10 or 20 µL L–1) to the transport water is advisable for the transportation of silver catfish, since both concentrations of these substances increased the levels of NPSH antioxidant and decreased the TBARS levels in the liver. In addition, the lower liver levels of glycogen and lactate in these groups and lower AChE activity in the brain (EOL 10 or 20 µL L–1) compared to the control group indicate that the energetic metabolism and neurotransmission were lower after administration of anesthetics, contributing to the maintenance of homeostasis and sedation status.

          Translated abstract

          Resumo O transporte de peixes vivos é uma prática rotineira na aquicultura e constitui uma fonte considerável de estresse para os animais. A adição de anestésicos na água utilizada para o transporte de peixes pode prevenir ou mitigar os efeitos nocivos do estresse de transporte. Este estudo investigou os efeitos da adição de eugenol (EUG) (1,5 ou 3,0 mL L–1) e óleo essencial de Lippia alba (OEL) (10 ou 20 mL L–1) em parâmetros metabólicos (níveis de glicogênio, lactato e proteína total) no fígado e músculos, a atividade da acetilcolinesterase (AChE) no músculo e no cérebro, e os níveis de proteína carbonil (PC), ácido tiobarbitúrico (TBARS) e grupos tióis não proteicos (NPSH) e atividade da glutationa-S-transferase no fígado de jundiás (Rhamdia quelen; Quoy e Gaimard, 1824) transportados por quatro horas em sacos plásticos (densidade de carga de 169,2 g L–1). A adição de várias concentrações de EUG (1,5 ou 3,0 mL L–1) e OEL (10 ou 20 mL L–1) na água de transporte é aconselhável para o transporte do jundiá, uma vez que ambas concentrações destas substâncias aumentaram os níveis do antioxidante NPSH e diminuíram os níveis de TBARS no fígado. Além disso, os níveis mais baixos de glicogénio e lactato no fígado destes grupos e menor atividade de AChE no cérebro (EOL 10 ou 20 mL L–1) em comparação com o grupo controle indicam que o metabolismo energético e neurotransmissão foram menores após a administração dos anestésicos, contribuindo para a manutenção do estado de homeostase e sedação.

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          Reviews in Fish Biology and Fisheries, 9(3), 211-268
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            Resveratrol attenuates mitochondrial oxidative stress in coronary arterial endothelial cells.

            The production of hyperglycemia-induced mitochondrial reactive oxygen species (mtROS) is a key event in the development of diabetic complications. Because resveratrol, a naturally occurring polyphenol, has been reported to confer vasoprotection, improving endothelial function and preventing complications of diabetes, we investigated the effect of resveratrol on mtROS production in cultured human coronary arterial endothelial cells (CAECs). The measurement of MitoSox fluorescence showed that resveratrol attenuates both steady-state and high glucose (30 mM)-induced mtROS production in CAECs, an effect that was prevented by the knockdown of the protein deacetylase silent information regulator 2/sirtuin 1 (SIRT1), an intracellular target of resveratrol. An overexpression of SIRT1 mimicked the effects of resveratrol, attenuating mtROS production. Similar results were obtained in CAECs transfected with mitochondria-targeted H(2)O(2)-sensitive HyPer-Mito fluorescent sensor. Amplex red assay showed that resveratrol and SIRT1 overexpression significantly reduced cellular H(2)O(2) levels as well. Resveratrol upregulated MnSOD expression and increased cellular GSH content in a concentration-dependent manner (measured by HPLC coulometric analysis). These effects were attenuated by SIRT1 knockdown and mimicked by SIRT1 overexpression. We propose that resveratrol, via a pathway that involves the activation of SIRT1 and the upregulation of antioxidant defense mechanisms, attenuates mtROS production, suggesting the potential for new treatment approaches targeting endothelial mitochondria in metabolic diseases.
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              Anaesthetic and Sedative Techniques for Aquatic Animals

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                bjb
                Brazilian Journal of Biology
                Braz. J. Biol.
                Instituto Internacional de Ecologia (São Carlos, SP, Brazil )
                1519-6984
                1678-4375
                May 2017
                : 77
                : 4
                : 696-702
                Affiliations
                [05] Santa Maria Rio Grande do Sul orgnameUniversidade Federal de Santa Maria orgdiv1Departamento de Farmácia Industrial Brazil
                [03] Faro orgnameUniversidade do Algarve orgdiv1Centro de Ciências do Mar Portugal
                [01] Santa Maria Rio Grande do Sul orgnameUniversidade Federal de Santa Maria orgdiv1Departamento de Fisiologia e Farmacologia Brazil
                [02] Frederico Westphalen Rio Grande do Sul orgnameInstituto Federal de Educação, Ciência e Tecnologia Farroupilha Brazil
                [04] Santa Maria Rio Grande do Sul orgnameUniversidade Federal de Santa Maria orgdiv1Departamento de Química Brazil
                Article
                S1519-69842017000400696
                10.1590/1519-6984.16515
                28492807
                ab0989b9-624a-4e60-ad2c-fa38ccd0dd9a

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 07 October 2015
                : 20 July 2016
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 43, Pages: 7
                Product

                SciELO Brazil


                anesthetic,metabolism,stress,fish transportation,anestésico,metabolismo,estresse,transporte de peixes

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