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      • Record: found
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      Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis

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      Publication date (Electronic, pub):
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      Journal: microPublication Biology
      Publisher: Caltech Library

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          Abstract

          METTL3 , the enzyme that catalyzes the m 6A RNA modification in Drosophila is highly conserved and essential in various eukaryotic organisms. Mettl3 and its homologs have been linked to biological processes such as gametogenesis. We focused on characterizing the role of METTL3 in Drosophila spermatogenesis. We used the Gal4-UAS system to ubiquitously knockdown Mettl3 in both somatic cyst cells and germline cells. Using immunostaining and confocal microscopy, we found spermatid bundles mislocalize in testes that contain the morphologically abnormal swollen apical tip. Our result suggests Mettl3 knockdown using TRiP.GL01126 results in spermatogenesis aberrations.

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          Epitranscriptomic influences on development and disease

          Phillip J Hsu, Hailing Shi, Chuan Sheng He (2017)
          RNA contains over 150 types of chemical modifications. Although many of these chemical modifications were discovered several decades ago, their functions were not immediately apparent. Discoveries of RNA demethylases, along with advances in mass spectrometry and high-throughput sequencing techniques, have caused research into RNA modifications to progress at an accelerated rate. Post-transcriptional RNA modifications make up an epitranscriptome that extensively regulates gene expression and biological processes. Here, we present an overview of recent advances in the field that are shaping our understanding of chemical modifications, their impact on development and disease, and the dynamic mechanisms through which they regulate gene expression.
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            Investigating spermatogenesis in Drosophila melanogaster.

            Rafael Demarco, Åsmund H. Eikenes, Kaisa Haglund … (2014)
            The process of spermatogenesis in Drosophila melanogaster provides a powerful model system to probe a variety of developmental and cell biological questions, such as the characterization of mechanisms that regulate stem cell behavior, cytokinesis, meiosis, and mitochondrial dynamics. Classical genetic approaches, together with binary expression systems, FRT-mediated recombination, and novel imaging systems to capture single cell behavior, are rapidly expanding our knowledge of the molecular mechanisms regulating all aspects of spermatogenesis. This methods chapter provides a detailed description of the system, a review of key questions that have been addressed or remain unanswered thus far, and an introduction to tools and techniques available to probe each stage of spermatogenesis.
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              N6-adenosine methylation in mRNA: substrate specificity and enzyme complexity.

              Suguna P. Narayan, J Bokar, G Shambaugh … (1993)
              The N6-methylation of internal adenosine residues is a common post-transcriptional modification of eukaryotic pre-mRNA sequences. An in vitro methylation system which retains the precise selectivity of in vivo methylation sites has been used to further define the nature of RNA site recognition. In addition to short consensus sequences, other structural features or context effects contribute to the selection of methylation sites in pre-mRNAs. Partial purification of the mRNA N6-adenosine methyltransferase revealed unexpected levels of complexity. The methyltransferase is composed of three separate components with molecular masses of 30, 200 and 875 kDa, respectively. These components are readily separated under non-denaturing conditions and each is required for mRNA methylation activity.
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                Author and article information

                Journal
                Journal ID (nlm-ta): MicroPubl Biol
                Journal ID (iso-abbrev): MicroPubl Biol
                Title: microPublication Biology
                Publisher: Caltech Library
                ISSN (Electronic): 2578-9430
                Publication date (Electronic, pub): 18 January 2022
                Publication date (Electronic, collection): 2022
                Volume: 2022
                Electronic Location Identifier: 10.17912/micropub.biology.000511
                Affiliations
                [1 ] Susquehanna University
                Author notes
                [§ ] Correspondence to: Antonio L Rockwell ( Rockwell@ 123456susqu.edu )

                LR: Data curation, Writing - original draft, Writing - review and editing, Formal analysis, Investigation

                AR: Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Writing - review and editing, Visualization, Validation

                Article
                DOI: 10.17912/micropub.biology.000511
                PMC ID: 8767421
                SO-VID: aab2463e-7bf9-4589-8a6d-859c9b9921e4
                Copyright © Copyright: © 2022 by the authors
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                Date received : 31 August 2021
                Date revision received : 02 January 2022
                Date accepted : 07 January 2022
                Categories
                Subject: New Finding
                Subject: Phenotype Data
                Subject: Drosophila

                Data availability:

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