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      Photosynthetic index and nitrogen assimilation in rapeseed seedlings transplanted in soil with ammonium glufosinate Translated title: Índice fotossintético e assimilação de nitrogênio em plântulas de colza transplantadas em resposta a resíduos de glufosinato presentes no solo

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          Abstract

          ABSTRACT: Herbicide application is an effective weed control method for mitigating crop yield loss; however, herbicide overuse can cause toxicity in non-target plants. The present study evaluated the effects of glufosinate at recommended dose for agricultural application (0.45 kg ha-1) and at overuse dose (0.90 kg ha-1) glufosinate application on photosynthetic performance and nitrogen assimilation of the rapeseed varieties D148 and Zhongshuang 11 (ZS11). Both glufosinate concentrations significantly decreased the content of chlorophyll and nitrogenous compounds, except free proline, and the activity of glutamine synthetase and glutamate synthase, and increased the activity of glutamic acid dehydrogenase in both varieties. When the concentration of glyphosate was 0.45kg ha-1, the nitrogen assimilation of the two varieties decreased, which indicated that the recommended dosage inhibited the nitrogen assimilation of the two varieties; however, the increase of net photosynthetic rate of D148 and the decrease of that of ZS11 mean that D148 is more tolerant to the recommended dose of glyphosate than ZS11. The 0.90 kg ha-1 dosage was toxic to both rapeseed varieties. Overall, our results indicated that herbicide overuse inhibited the photosynthetic rate and nitrogen assimilation in rapeseed seedlings, and it is essential to apply a suitable glufosinate dose based on the variety grown to minimize adverse effects on crops and environment.

          Translated abstract

          RESUMO: A aplicação de herbicidas é um método eficaz de controle de ervas daninhas para mitigar a perda de produtividade das culturas. No entanto, o uso excessivo de herbicidas pode causar toxicidade em plantas não alvo. O presente estudo avaliou os efeitos da dose recomendada para aplicação agrícola (0.45 kg ha-1) e dose excessiva (0.90 kg ha-1) de glufosinato no desempenho fotossintético e assimilação de nitrogênio das variedades de colza D148 e Zhongshuang 11 (ZS11). Ambas as concentrações de glutamato diminuíram significativamente o teor de clorofila e compostos azotados, exceto a prolina livre, e a atividade de síntese da glutamina e de síntese de glutamato, e aumentaram a atividade de desidrogenase do ácido glutâmico em ambas as variedades. Quando a concentração de glifosato foi 0.45 kg ha-1, a assimilação de azoto das duas variedades diminuiu, o que indicou que a dosagem recomendada de glifosato inibiu a assimilação de azoto das duas variedades de colza. Entretanto, a taxa fotosintética líquida do D148 aumentou enquanto o do ZS11 diminuiu, o que significa que o D148 é mais tolerante a dose recomendada de glifosato do que o ZS11. A dose de 0.90 kg ha-1 de glifosato foi prejudicial para as mudas de duas variedades de colza. Em geral, os nossos resultados indicam que o uso excessivo de glufosinato inibe a taxa fotossintética e a assimilação de nitrogênio em mudas de colza, sendo essencial aplicar uma dose adequada deste herbicida com base na variedade cultivada para minimizar os efeitos adversos nas culturas e no meio ambiente.

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          Most cited references36

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          The increasing importance of herbicides in worldwide crop production.

          Herbicide use is increasingly being adopted around the world. Many developing countries (India, China, Bangladesh) are facing shortages of workers to hand weed fields as millions of people move from rural to urban areas. In these countries, herbicides are far cheaper and more readily available than labor for hand weeding. History shows that in industrializing countries in the past, including the United States, Germany, Japan and South Korea, the same phenomenon has occurred-as workers have left agriculture, herbicides have been adopted. It is inevitable that herbicide use will increase in sub-Saharan Africa, not only because millions of people are leaving rural areas, creating shortages of hand weeders, but also because of the need to increase crop yields. Hand weeding has never been a very efficient method of weed control-often performed too late and not frequently enough. Uncontrolled weeds have been a major cause of low crop yields in sub-Saharan Africa for a long time. In many parts of the world, herbicides are being increasingly used to replace tillage in order to improve environmental conditions. In comparison with tillage, herbicide use reduces erosion, fuel use, greenhouse gas emissions and nutrient run-off and conserves water.
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            Resistance of Common Groundsel to Simazine and Atrazine

            G Ryan (1970)
            Failure to control common groundsel (Senecio vulgaris L.) was observed in 1968 in a nursery where 2-chloro-4,6-bis(ethylamino)- s -triazine (simazine) or 2-chloro-4-(ethylamino)-6-(isopropylamino)- s -triazine (atrazine) had been used once or twice annually since 1958. Seedlings from seed collected at that location were not controlled by pre-emergence applications of simazine or atrazine at rates up to 17.92 kg/ha. Seedlings from a seed source where triazine herbicides had not been in continuous use were completely controlled by 1.12 or 2.24 kg/ha of either chemical, and partially controlled by 0.28 to 0.56 kg/ha. Postemergence applications of atrazine at 1.12, 2.24, or 4.48 kg/ha did not affect common groundsel from the nursery source, but killed small seedlings from the other source. Preemergence applications of four other herbicides were equally effective against seedlings from both sources.
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              Genotoxicity assessment of acute exposure of chlorpyrifos to freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis.

              Chlorpyrifos (O,O-diethyl O-3,5,6-trichloro-2-pyridylphosphorothioate) is one of the organophosphate pesticides widely used in agricultural practices throughout world and irreversible inhibitor of cholinesterase in all animal species. Limited efforts have been made to study acute genotoxic effects of chlorpyrifos (CPF) in different tissues of fish using genotoxic biomarkers. Therefore, the present investigation was aimed to study the induction of DNA damage by CPF in freshwater teleost fish Channapunctatus using micronucleus assay (MN assay) and alkaline single-cell gel electrophoresis (comet assay). The value of LC(50) - 96 h of CPF was determined as 811.98 microgl(-1) for C. punctatus, in a semi-static system and on the basis of LC(50) value three acute concentrations viz., 203, 406 and 609 microgl(-1) were determined. The fishes were exposed to the different concentrations of CPF for 96 h and samplings were done at regular intervals for assessment of the MN frequencies and DNA damage. In general, significant effects (P<0.01) from both concentrations and time of exposure were observed in exposed fishes. It was found that the micronucleus induction was highest on 96 h at all concentrations in the peripheral blood. Similar trend was observed for the DNA damage measured in terms of the percentage of tail DNA in the lymphocyte and gill cells. This study explored the combined use of micronucleus assay and comet assay for in vivo laboratory studies using fresh water fish for screening the genotoxic potential of xenobiotics.
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                Author and article information

                Journal
                cr
                Ciência Rural
                Cienc. Rural
                Universidade Federal de Santa Maria (Santa Maria, RS, Brazil )
                0103-8478
                1678-4596
                2020
                : 50
                : 4
                : e20180911
                Affiliations
                [1] Chongqing Chongqing orgnameSouthwest University orgdiv1College of Agronomy and Biotechnology China
                Article
                S0103-84782020000400251 S0103-8478(20)05000400251
                10.1590/0103-8478cr20180911
                aaa6d210-7085-44d0-a728-116e2cd57129

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 10 December 2019
                : 11 June 2018
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 48, Pages: 0
                Product

                SciELO Brazil

                Self URI: Full text available only in PDF format (EN)
                Categories
                Soil Science

                Soil
                redidual de glufosinato,colza,mudas transplantadas,fotossíntese,assimilação de nitrogênio,residual glufosinate,rapeseed,seedling transplanted,photosynthesis,nitrogen assimilation

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