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      A brief review of forensically important flesh flies (Diptera: Sarcophagidae)

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          ABSTRACT

          Forensic entomology could provide valuable data for the minimum postmortem interval (PMI min) estimation and other relevant information, such as causes and circumstances of death. Some representatives of flesh flies are one of the dominant necrophagous insects during early stages of decomposition, demonstrating unique biological characteristics compared with other necrophagous flies. Moreover, they lead to global health concerns as carriers of various pathogenic micro-organisms, and dominantly result in the traumatic myiasis. Thus, sarcophagid flies are considered important in decomposition processes for PMI min estimation. However, the utility of sarcophagid flies has been seriously hampered by limited ecological, biological and taxonomic knowledge of them. The aim of this paper is to provide a brief review on the species, distribution and biological habit of forensically important sarcophagid flies. In addition, the relation between traumatic myiasis and flesh flies, molecular identification methods and developmental pattern of flesh flies are summarized.

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          Forensic entomology: applications and limitations.

          Forensic entomology is the science of collecting and analysing insect evidence to aid in forensic investigations. Its main application is in the determination of the minimum time since death in cases of suspicious death, either by estimating the age of the oldest necrophagous insects that developed on the corpse, or by analysing the insect species composition on the corpse. In addition, toxicological and molecular examinations of these insects may help reveal the cause of death or even the identity of a victim, by associating a larva with its last meal, for example, in cases where insect evidence is left at a scene after human remains have been deliberately removed. Some fly species can develop not only on corpses but on living bodies too, causing myiasis. Analysis of larvae in such cases can demonstrate the period of neglect of humans or animals. Without the appropriate professional collection of insect evidence, an accurate and convincing presentation of such evidence in court will be hampered or even impossible. The present paper describes the principles and methods of forensic entomology and the optimal techniques for collecting insect evidence.
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            Initial Studies on Insect Succession on Carrion in Southwestern British Columbia

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              Patterns of evolution of mitochondrial cytochrome c oxidase I and II DNA and implications for DNA barcoding.

              DNA barcoding has focused increasing attention on the use of specific regions of mitochondrial cytochrome c oxidase I and II genes (COI-COII) to diagnose and delimit species. However, our understanding of patterns of molecular evolution within these genes is limited. Here we examine patterns of nucleotide divergence in COI-COII within species and between species pairs of Lepidoptera and Diptera using a sliding window analysis. We found that: (1) locations of maximum divergence within COI-COII were highly variable among taxa surveyed in this study; (2) there was major overlap in divergence within versus between species, including within individual COI-COII profiles; (3) graphical DNA saturation analysis showed variation in percent nucleotide transitions throughout COI-COII and only limited association with levels of DNA divergence. Ultimately, no single optimally informative 600 bp location was found within the 2.3 kb of COI-COII, and the DNA barcoding region was no better than other regions downstream in COI. Consequently, we recommend that researchers should maximize sequence length to increase the probability of sampling regions of high phylogenetic informativeness, and to minimize stochastic variation in estimating total divergence.
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                Author and article information

                Journal
                Forensic Sci Res
                Forensic Sci Res
                TFSR
                tfsr20
                Forensic sciences research
                Taylor & Francis
                2096-1790
                2471-1411
                2018
                22 March 2018
                : 3
                : 1 , Special Issue on Forensic Entomology
                : 16-26
                Affiliations
                [a ]Department of Forensic Science, School of Basic Medical Sciences, Central South University , Changsha, China
                [b ]Department of Forensic Medicine, Shantou University Medical College , Shantou, China
                [c ]Department of Forensic Medicine, Guizhou Police Officer Vocational College , Guiyang, China
                [d ]Department of Forensic Medicine, Hainan Medical University , Haikou, China
                Author notes
                Author information
                http://orcid.org/0000-0002-4916-7368
                Article
                1432099
                10.1080/20961790.2018.1432099
                6197121
                30483648
                a96e88b8-3915-40f1-80f9-473b5b66a3f0
                © 2018 The Author(s). Published by Taylor & Francis Group on behalf of the Academy of Forensic Science.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 26 September 2017
                : 21 January 2018
                Page count
                Figures: 0, Tables: 3, References: 132, Pages: 11
                Funding
                Funded by: National Natural Science Foundation of China 10.13039/501100001809
                Award ID: 81302615, 81772026
                Funded by: National Natural Science Foundation of Hunan Province
                Award ID: 2017JJ3512
                This study is supported by the National Natural Science Foundation of China [grant numbers 81302615 and 81772026]; the National Natural Science Foundation of Hunan Province [grant number 2017JJ3512].
                Categories
                Review

                forensic science,forensic entomology,sarcophagid flies,decomposition,biology,distribution

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