Draft Genome Sequence of Planomonospora sphaerica JCM9374, a Rare Actinomycete

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Planomonospora sphaerica is a rare actinomycete that is a potential antibiotic producer. Here, we report the draft genome sequence of P. sphaerica strain JCM9374. This is the first genome report of a bacterium belonging to the genus Planomonospora. The genome information of P. sphaerica will contribute to studies on the structure and function of antibiotics.

Related collections

Most cited references 2

Record: found
Abstract: found
Article: found

Is Open Access

The khmer software package: enabling efficient nucleotide sequence analysis

Michael R. Crusoe, Hussien Alameldin, Sherine Awad … (2015)

The khmer package is a freely available software library for working efficiently with fixed length DNA words, or k-mers. khmer provides implementations of a probabilistic k-mer counting data structure, a compressible De Bruijn graph representation, De Bruijn graph partitioning, and digital normalization. khmer is implemented in C++ and Python, and is freely available under the BSD license at https://github.com/dib-lab/khmer/.

0 comments Cited 166 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Cloning and analysis of the planosporicin lantibiotic biosynthetic gene cluster of Planomonospora alba.

David Sherwood, A Hesketh, Mervyn Bibb (2013)

The increasing prevalence of antibiotic resistance in bacterial pathogens has renewed focus on natural products with antimicrobial properties. Lantibiotics are ribosomally synthesized peptide antibiotics that are posttranslationally modified to introduce (methyl)lanthionine bridges. Actinomycetes are renowned for their ability to produce a large variety of antibiotics, many with clinical applications, but are known to make only a few lantibiotics. One such compound is planosporicin produced by Planomonospora alba, which inhibits cell wall biosynthesis in Gram-positive pathogens. Planosporicin is a type AI lantibiotic structurally similar to those which bind lipid II, the immediate precursor for cell wall biosynthesis. The gene cluster responsible for planosporicin biosynthesis was identified by genome mining and subsequently isolated from a P. alba cosmid library. A minimal cluster of 15 genes sufficient for planosporicin production was defined by heterologous expression in Nonomuraea sp. strain ATCC 39727, while deletion of the gene encoding the precursor peptide from P. alba, which abolished planosporicin production, was also used to confirm the identity of the gene cluster. Deletion of genes encoding likely biosynthetic enzymes identified through bioinformatic analysis revealed that they, too, are essential for planosporicin production in the native host. Reverse transcription-PCR (RT-PCR) analysis indicated that the planosporicin gene cluster is transcribed in three operons. Expression of one of these, pspEF, which encodes an ABC transporter, in Streptomyces coelicolor A3(2) conferred some degree of planosporicin resistance on the heterologous host. The inability to delete these genes from P. alba suggests that they play an essential role in immunity in the natural producer.

0 comments Cited 21 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): Genome Announc

Journal ID (iso-abbrev): Genome Announc

Journal ID (hwp): ga

Journal ID (pmc): ga

Journal ID (publisher-id): GA

Title: Genome Announcements

Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )

ISSN (Electronic): 2169-8287

Publication date (Electronic): 4 August 2016

Publication date Collection: Jul-Aug 2016

Volume: 4

Issue: 4

Electronic Location Identifier: e00779-16

Affiliations

[a ]Instrumental Research Support Office, Research Institute of Green Science and Technology, Shizuoka University, Suruga-ku, Shizuoka, Japan

[b ]Department of Science, Graduate School of Integrated Science and Technology, Shizuoka University, Suruga-ku, Shizuoka, Japan

[c ]Center for Bioscience Research and Education, Utsunomiya University, Utsunomiya, Tochigi, Japan

[d ]Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Suruga-ku, Shizuoka, Japan

Author notes

Address correspondence to Shinya Kodani, kodani.shinya@ 123456shizuoka.ac.jp .

Article

Publisher ID: genomeA00779-16

DOI: 10.1128/genomeA.00779-16

PMC ID: 4974322

PubMed ID: 27492001

SO-VID: a8f98517-0575-4cb8-954b-462022d75529

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

History

Date received : 9 June 2016

Date accepted : 10 June 2016

Page count

Figures: 0, Tables: 0, Equations: 0, References: 8, Pages: 2, Words: 1122

Funding

Funded by: Japan Society for the Promotion of Science (JSPS) http://dx.doi.org/10.13039/501100001691

Award ID: 16K01913

Award Recipient : Shinya Kodani

Custom metadata

cover-date July/August 2016

ScienceOpen disciplines: Genetics

Data availability:

ScienceOpen disciplines: Genetics

Comments

Comment on this article

scite_

Cited by 4

See all cited by

Most referenced authors 410

See all reference authors