LINC01094/miR-577 axis regulates the progression of ovarian cancer

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Abstract

Background

Long intergenic non-coding RNA 01094 (LINC01094) is probably a novel regulator in cancer biology. This study aimed to probe into the function and mechanism of LINC01094 in ovarian cancer (OC).

Methods

Quantitative real-time polymerase chain reaction (qRT-PCR) assay was utilized to measure LINC01094 and miR-577 expressions in OC tissues and cell lines. Western blot was used to examine the expressions of epithelial-mesenchymal transition (EMT)-related proteins, β-catenin, c-Myc and cyclin D1. Cell counting kit-8 (CCK-8) and Transwell assays were used to detect the proliferation, migration and invasion of SKOV3 and 3AO cells, respectively. Eventually, dual-luciferase reporter gene assay was employed to detect the regulatory relationship between miR-577 and LINC01094.

Results

LINC01094 expression was elevated in OC tissues and cell lines. High LINC01094 expression was associated with higher FIGO stage, lymph node metastasis and the shorter overall survival rate in patients with OC. Meanwhile, LINC01094 knockdown inhibited OC cell proliferation, migration, invasion and EMT. In addition, miR-577 was demonstrated to be a direct downstream target of LINC01094 in OC and inhibition of miR-577 reversed the biological effects of LINC01094 knockdown on OC cells. Additionally, LINC01094 / miR-577 axis regulated the expressions of β-catenin, c-Myc and cyclin D1 in OC cells.

Conclusion

LINC01094 promotes the proliferation, migration, invasion and EMT of OC cells by adsorbing miR-577.

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Most cited references 35

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MicroRNA therapeutics: towards a new era for the management of cancer and other diseases

Rajesha Rupaimoole, Frank J Slack (2017)

MicroRNAs (miRNAs) are small non-coding RNAs that can modulate mRNA expression. Insights into the roles of miRNAs in development and disease have led to the development of new therapeutic approaches that are based on miRNA mimics or agents that inhibit their functions (antimiRs), and the first such approaches have entered the clinic. This Review discusses the role of different miRNAs in cancer and other diseases, and provides an overview of current miRNA therapeutics in the clinic.

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Functional Classification and Experimental Dissection of Long Noncoding RNAs

Florian Kopp, Joshua Mendell (2018)

Over the last decade, it has been increasingly demonstrated that the genomes of many species are pervasively transcribed, resulting in the production of numerous long noncoding RNAs (lncRNAs). At the same time, it is now appreciated that many types of DNA regulatory elements, such as enhancers and promoters, regularly initiate bidirectional transcription. Thus, discerning functional noncoding transcripts from a vast transcriptome is a paramount priority, and challenge, for the lncRNA field. In this review, we aim to provide a conceptual and experimental framework for classifying and elucidating lncRNA function. We categorize lncRNA loci into those that regulate gene expression in cis versus those that perform functions in trans , and propose an experimental approach to dissect lncRNA activity based on these classifications. These strategies to further understand lncRNAs promise to reveal new and unanticipated biology, with great potential to advance our understanding of normal physiology and disease.

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Ovarian cancer statistics, 2018

Lindsey A Torre, Britton Trabert, Carol DeSantis … (2018)

In 2018, there will be approximately 22,240 new cases of ovarian cancer diagnosed and 14,070 ovarian cancer deaths in the United States. Herein, the American Cancer Society provides an overview of ovarian cancer occurrence based on incidence data from nationwide population-based cancer registries and mortality data from the National Center for Health Statistics. The status of early detection strategies is also reviewed. In the United States, the overall ovarian cancer incidence rate declined from 1985 (16.6 per 100,000) to 2014 (11.8 per 100,000) by 29% and the mortality rate declined between 1976 (10.0 per 100,000) and 2015 (6.7 per 100,000) by 33%. Ovarian cancer encompasses a heterogenous group of malignancies that vary in etiology, molecular biology, and numerous other characteristics. Ninety percent of ovarian cancers are epithelial, the most common being serous carcinoma, for which incidence is highest in non-Hispanic whites (NHWs) (5.2 per 100,000) and lowest in non-Hispanic blacks (NHBs) and Asians/Pacific Islanders (APIs) (3.4 per 100,000). Notably, however, APIs have the highest incidence of endometrioid and clear cell carcinomas, which occur at younger ages and help explain comparable epithelial cancer incidence for APIs and NHWs younger than 55 years. Most serous carcinomas are diagnosed at stage III (51%) or IV (29%), for which the 5-year cause-specific survival for patients diagnosed during 2007 through 2013 was 42% and 26%, respectively. For all stages of epithelial cancer combined, 5-year survival is highest in APIs (57%) and lowest in NHBs (35%), who have the lowest survival for almost every stage of diagnosis across cancer subtypes. Moreover, survival has plateaued in NHBs for decades despite increasing in NHWs, from 40% for cases diagnosed during 1992 through 1994 to 47% during 2007 through 2013. Progress in reducing ovarian cancer incidence and mortality can be accelerated by reducing racial disparities and furthering knowledge of etiology and tumorigenesis to facilitate strategies for prevention and early detection. CA Cancer J Clin 2018;68:284-296. © 2018 American Cancer Society.

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Author and article information

Contributors

Ping Zhang:

ORCID: http://orcid.org/0000-0003-3359-8320

iamzhangping1978@163.com

Journal

Journal ID (nlm-ta): J Ovarian Res

Journal ID (iso-abbrev): J Ovarian Res

Title: Journal of Ovarian Research

Publisher: BioMed Central (London )

ISSN (Electronic): 1757-2215

Publication date (Electronic): 17 October 2020

Publication date PMC-release: 17 October 2020

Publication date Collection: 2020

Volume: 13

Electronic Location Identifier: 122

Affiliations

[1 ]Department of Obstetrics and Gynecology, Zibo Hospital of Integrated Traditional Chinese and Western Medicine, Zibo, Shandong Province China

[2 ]GRID grid.415946.b, Department of Reproductive Medicine, Linyi People’s Hospital, ; Fenghuang Street No. 233, Hedong District, Linyi, Shandong Province China

[3 ]Department of Obstetrics and Gynecology, Yantai Yeda Hospital, Yantai, Shandong Province China

[4 ]Department of Gynecology, Qingdao Women’s and Children’s Hospital, Qingdao, Shandong Province China

Author information

Ping Zhang http://orcid.org/0000-0003-3359-8320

Article

Publisher ID: 721

DOI: 10.1186/s13048-020-00721-9

PMC ID: 7568364

PubMed ID: 33069244

SO-VID: a34ce666-ca00-4520-86e1-32a16dc0a624

License:

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

History

Date received : 5 May 2020

Date accepted : 17 September 2020

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ScienceOpen disciplines: Obstetrics & Gynecology

Keywords: linc01094,mir-577,ovarian cancer,cancer progression

Data availability:

ScienceOpen disciplines: Obstetrics & Gynecology

Keywords: linc01094, mir-577, ovarian cancer, cancer progression

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LINC01094/miR-577 axis regulates the progression of ovarian cancer

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Abstract

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Karger: Oncology

Most cited references 35

MicroRNA therapeutics: towards a new era for the management of cancer and other diseases

Functional Classification and Experimental Dissection of Long Noncoding RNAs

Ovarian cancer statistics, 2018

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