The seeds of Arabidopsis thaliana become encapsulated by a layer of mucilage when imbibed. This polysaccharide-rich hydrogel is constituted of two layers, an outer layer that can be easily extracted with water and an inner layer that must be examined in situ in order to study its properties and structure in a non-destructive manner or disintegrated through hydrolysis or physical means in order to analyze its constituents. Mucilage production is an adaptive trait and we have exploited 19 natural accessions previously found to have atypical and varied outer mucilage characteristics. A detailed study using biochemical, histological and Time-Domain NMR analyses has been used to generate three related datasets covering 33 traits measured in four biological replicates. This data will be a rich resource for genetic, biochemical, structural and functional analyses investigating mucilage constituent polysaccharides or their role as adaptive traits.
Measurement(s) | intrinsic viscosity • galacturonic acid • seed and inner mucilage layer width • Transverse Spin Relaxation Time • Transverse Spin Relaxation intensity • mucilage • neutral sugar content • mass per seed • sample mass |
Technology Type(s) | size-exclusion chromatography • colorimetry • immunolabeling method • confocal microscopy • nuclear magnetic resonance spectroscopy • Time-domain NMR • biochemical, histological and Time-Domain NMR analyses • biochemical method using orcinol • microbalance |
Sample Characteristic - Organism | Arabidopsis thaliana |
Machine-accessible metadata file describing the reported data: 10.6084/m9.figshare.13320593