Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay

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Abstract

Herein, we described a novel plasmonic CRISPR Cas12a assay for the visual, colorimetric detection of grapevine viral infections. Our assay generates rapid and specific colorimetric signals for nucleic acid amplicons by combining the unique target-induced incriminate single-stranded DNase activity of Cas12a with plasmon coupling of DNA functionalized gold nanoparticles. The practical applicability of our plasmonic assay was successfully demonstrated through the detection of emerging red-blotch viral infections in grapevine samples collected from commercial vineyards.

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Journal

Title: Analytical Chemistry

Abbreviated Title: Anal. Chem.

Publisher: American Chemical Society (ACS)

ISSN (Print): 0003-2700

ISSN (Electronic): 1520-6882

Publication date Created: September 05 2019

Publication date (Electronic): September 05 2019

Affiliations

[1 ]College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, P.R. China

[2 ]Department of Chemistry, Centre for Biotechnology, Brock University, St. Catharines, Ontario L2S 3A1, Canada

[3 ]Department of Cell Biology, National Research Centre, Cairo 12622, Egypt

[4 ]Cool Climate Oenology and Viticulture Institute, Brock University, St. Catharines, Ontario L2S 3A1, Canada

Article

DOI: 10.1021/acs.analchem.9b03545

PubMed ID: 31478642

SO-VID: 9ebf8cf7-97bb-43c5-9029-bb74cee8f112

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