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      Anti-Leptospira spp and anti-Brucella spp antibodies in humans from rural area of Monte Negro municipality, state of Rondônia, Brazilian Western Amazon Translated title: Anticorpos anti-Leptospira spp e anti-Brucella spp em humanos da área rural do município de Monte Negro, estado de Rondônia, Amazônia Ocidental Brasileira

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          Abstract

          Sera from 276 humans living in 71 farms located in Monte Negro Municipality, RO., Western Amazon, Brazil were examined for anti-Leptospira spp antibodies by Microscopic Agglutination Test and for anti-Brucella spp antibodies by Tube Agglutination Test. Leptospira spp antibodies were detected in 28 (10.2%) of them with at least one positive case in 23 farms (32.4%). The most frequent leptospira serovars were Patoc (46.7%), Autumnalis (10.0%) and Shermani (10.0%). The proportion of positive males (14.5%) were higher than females (5.0%; P <0.05) and the contact with river water presented association with Leptospira spp infection (OR: 27; P=0.01). A total of 04 (1.4%) humans reacted against Brucella antigens; three farms (4.2%) presented at least one positive case of brucellosis.

          Translated abstract

          Foram avaliados soros de 276 humanos procedentes de 71 fazendas localizadas no município de Monte Negro, RO, pela Soroaglutinação Microscópica para verificar a presença de anticorpos anti-Leptospira spp e pela Soroaglutinação Lenta em Tubos para verificar a presença de anticorpos anti-Brucella spp. Anticorpos anti-Leptospira spp foram detectados em 28 (10,2%) humanos procedentes de 23 fazendas (32,4%). Os sorovares mais freqüentes foram Patoc (46,7%), Autumnalis (10,0%) e Shermani (10,0%). A positividade foi maior no sexo masculino (14,5%) que no feminino (5,0%; P < 0,05) e o contato com a água de rio apresentou associação com a infecção por Leptospira spp (OR: 27; P < 0,05). Quatro humanos (1,4%) reagiram contra antígenos de Brucella spp, com três (4,2%) fazendas apresentando pelo menos uma reação positiva para Brucella spp.

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          Applied Logistic Regression

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            Brucellosis: an overview.

            M Corbel (1997)
            Brucellosis remains a major zoonosis worldwide. Although many countries have eradicated Brucella abortus from cattle, in some areas Brucella melitensis has emerged as a cause of infection in this species as well as in sheep and goats. Despite vaccination campaigns with the Rev 1 strain, B. melitensis remains the principal cause of human brucellosis. Brucella suis is also emerging as an agent of infection in cattle, thus extending its opportunities to infect humans. The recent isolation of distinctive strains of Brucella from marine mammals has extended its ecologic range. Molecular genetic studies have demonstrated phylogenetic affiliation to Agrobacterium, Phyllobacterium, Ochrobactrum, and Rhizobium. Polymerase chain reaction and gene probe development may provide more effective typing methods. Pathogenicity is related to production of lipopolysaccharides containing a poly N-formyl perosamine O chain, CuZn superoxide dismutase, erythrlose phosphate dehydrogenase, stress-induced proteins related to intracellular survival, and adenine and guanine monophosphate inhibitors of phagocyte functions. Protective immunity is conferred by antibody to lipopolysaccharide and T-cell-mediated macrophage activation triggered by protein antigens. Diagnosis still centers on isolation of the organism and serologic test results, especially enzyme immunoassay, which is replacing other methods. Polymerase chain reaction is also under evaluation. Therapy is based on tetracyclines with or without rifampicin, aminoglycosides, or quinolones. No satisfactory vaccines against human brucellosis are available, although attenuated purE mutants appear promising.
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              Brucellosis in Brazil.

              This paper reviews the epidemiology of bovine, swine, ovine, caprine, and canine brucellosis in Brazil. The zoonotic aspects of Brucella infection in Brazil is also discussed. Emphasis is given to the new program for the control of brucellosis in cattle and buffaloes that is likely to provide important insights into the prospects and strategies for controlling brucellosis in developing countries. Copyright 2002 Elsevier Science B.V.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                bjm
                Brazilian Journal of Microbiology
                Braz. J. Microbiol.
                Sociedade Brasileira de Microbiologia (São Paulo )
                1678-4405
                March 2007
                : 38
                : 1
                : 93-96
                Affiliations
                [1 ] Agência Paulista de Tecnologia dos Agronegócios Brazil
                [2 ] Universidade de São Paulo Brazil
                [3 ] Universidade de São Paulo Brazil
                Article
                S1517-83822007000100019
                10.1590/S1517-83822007000100019
                9e09f3c9-116f-455f-bc00-7cc8913dc552

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1517-8382&lng=en
                Categories
                MICROBIOLOGY

                Microbiology & Virology
                Human,Leptospirosis,Brucellosis,Amazon,Brazil,Humanos,Leptospirose,Brucelose,Amazônia,Brasil
                Microbiology & Virology
                Human, Leptospirosis, Brucellosis, Amazon, Brazil, Humanos, Leptospirose, Brucelose, Amazônia, Brasil

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