Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

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Abstract

The purpose of this work is to provide an exhaustive overview of the emerging biosensor technologies for the detection of analytes of interest for food, environment, security, and health. Over the years, biosensors have acquired increasing importance in a wide range of applications due to synergistic studies of various scientific disciplines, determining their great commercial potential and revealing how nanotechnology and biotechnology can be strictly connected. In the present scenario, biosensors have increased their detection limit and sensitivity unthinkable until a few years ago. The most widely used biosensors are optical-based devices such as surface plasmon resonance (SPR)-based biosensors and fluorescence-based biosensors. Here, we will review them by highlighting how the progress in their design and development could impact our daily life.

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Most cited references 227

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Biosensing with plasmonic nanosensors.

Jeffrey N Anker, W. Hall, Olga Lyandres … (2008)

Recent developments have greatly improved the sensitivity of optical sensors based on metal nanoparticle arrays and single nanoparticles. We introduce the localized surface plasmon resonance (LSPR) sensor and describe how its exquisite sensitivity to size, shape and environment can be harnessed to detect molecular binding events and changes in molecular conformation. We then describe recent progress in three areas representing the most significant challenges: pushing sensitivity towards the single-molecule detection limit, combining LSPR with complementary molecular identification techniques such as surface-enhanced Raman spectroscopy, and practical development of sensors and instrumentation for routine use and high-throughput detection. This review highlights several exceptionally promising research directions and discusses how diverse applications of plasmonic nanoparticles can be integrated in the near future.

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Michael J. Berridge, Martin Bootman, H. Roderick (2003)

Ca2+ is a highly versatile intracellular signal that operates over a wide temporal range to regulate many different cellular processes. An extensive Ca2+-signalling toolkit is used to assemble signalling systems with very different spatial and temporal dynamics. Rapid highly localized Ca2+ spikes regulate fast responses, whereas slower responses are controlled by repetitive global Ca2+ transients or intracellular Ca2+ waves. Ca2+ has a direct role in controlling the expression patterns of its signalling systems that are constantly being remodelled in both health and disease.

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Electrochemical Biosensors - Sensor Principles and Architectures

Dorothee Grieshaber, Robert MacKenzie, Janos Vörös … (2008)

Quantification of biological or biochemical processes are of utmost importance for medical, biological and biotechnological applications. However, converting the biological information to an easily processed electronic signal is challenging due to the complexity of connecting an electronic device directly to a biological environment. Electrochemical biosensors provide an attractive means to analyze the content of a biological sample due to the direct conversion of a biological event to an electronic signal. Over the past decades several sensing concepts and related devices have been developed. In this review, the most common traditional techniques, such as cyclic voltammetry, chronoamperometry, chronopotentiometry, impedance spectroscopy, and various field-effect transistor based methods are presented along with selected promising novel approaches, such as nanowire or magnetic nanoparticle-based biosensing. Additional measurement techniques, which have been shown useful in combination with electrochemical detection, are also summarized, such as the electrochemical versions of surface plasmon resonance, optical waveguide lightmode spectroscopy, ellipsometry, quartz crystal microbalance, and scanning probe microscopy. The signal transduction and the general performance of electrochemical sensors are often determined by the surface architectures that connect the sensing element to the biological sample at the nanometer scale. The most common surface modification techniques, the various electrochemical transduction mechanisms, and the choice of the recognition receptor molecules all influence the ultimate sensitivity of the sensor. New nanotechnology-based approaches, such as the use of engineered ion-channels in lipid bilayers, the encapsulation of enzymes into vesicles, polymersomes, or polyelectrolyte capsules provide additional possibilities for signal amplification. In particular, this review highlights the importance of the precise control over the delicate interplay between surface nano-architectures, surface functionalization and the chosen sensor transducer principle, as well as the usefulness of complementary characterization tools to interpret and to optimize the sensor response.

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Author and article information

Contributors

Abel Santos: Role: Academic Editor

Journal

Journal ID (nlm-ta): Sensors (Basel)

Journal ID (iso-abbrev): Sensors (Basel)

Journal ID (publisher-id): sensors

Title: Sensors (Basel, Switzerland)

Publisher: MDPI

ISSN (Electronic): 1424-8220

Publication date (Electronic): 29 January 2021

Publication date Collection: February 2021

Volume: 21

Issue: 3

Electronic Location Identifier: 906

Affiliations

[1 ]Institute of Food Science, CNR Italy, 83100 Avellino, Italy; alessandra.camarca@ 123456isa.cnr.it (A.C.); antonio.varriale@ 123456isa.cnr.it (A.V.); alessandro.capo@ 123456isa.cnr.it (A.C.); angela.pennacchio@ 123456isa.cnr.it (A.P.); alessia.calabrese@ 123456isa.cnr.it (A.C.); cristina.giannattasio@ 123456isa.cnr.it (C.G.); carlos.murillo-almuzara@ 123456stud.sbg.ac.at (C.M.A.); maria.staiano@ 123456isa.cnr.it (M.S.)

[2 ]URT-ISA at Department of Biology, University of Naples Federico II, 80126 Napoli, Italy

Author notes

[* ]Correspondence: sabato.dauria@ 123456cnr.it ; Tel.: +39-082-529-9101

Article

Publisher ID: sensors-21-00906

DOI: 10.3390/s21030906

PMC ID: 7866296

PubMed ID: 33572812

SO-VID: 9b488f0e-b806-46c9-a920-cb7070c05dd8

License:

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

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Journal of Disability Research

Most cited references 227

Biosensing with plasmonic nanosensors.

Calcium signalling: dynamics, homeostasis and remodelling.

Electrochemical Biosensors - Sensor Principles and Architectures

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