Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin.

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Abstract

16S rRNA methyltransferases confer resistance to most aminoglycosides, but discriminating their activity from that of aminoglycoside-modifying enzymes (AMEs) is challenging using phenotypic methods. We demonstrate that arbekacin, an aminoglycoside refractory to most AMEs, can rapidly detect 16S methyltransferase activity in Enterobacteriaceae with high specificity using the standard disk susceptibility test.

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Journal

Journal ID (iso-abbrev): J Clin Microbiol

Title: Journal of clinical microbiology

Publisher: American Society for Microbiology

ISSN (Electronic): 1098-660X

ISSN (Print): 0095-1137

Publication date (Electronic): Jan 2016

Volume: 54

Issue: 1

Affiliations

[1 ] Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA patrick.t.mcgann4.ctr@mail.mil.

[2 ] Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.

[3 ] Infectious Diseases Service, Walter Reed National Military Medical Center, Bethesda, Maryland, USA.

[4 ] Division of Bacterial & Rickettsial Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.

Article

Publisher Item ID: JCM.02642-15

DOI: 10.1128/JCM.02642-15

PMC ID: 4702724

PubMed ID: 26537447

SO-VID: 99c78837-cee6-45a2-9338-1716d85b0595

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