Rapid and sustained homeostatic control of presynaptic exocytosis at a central synapse

Significance

Homeostatic mechanisms stabilize neural activity, and there are genetic links between homeostatic plasticity and neural disease. While homeostatic plasticity in the central nervous system (CNS) operates on relatively slow time scales of hours to days, activity-dependent forms of synaptic plasticity alter neural activity on much faster time scales. It is unclear if homeostatic plasticity stabilizes CNS synapses on rapid time scales. Here, we uncovered that cerebellar synapses stabilize transmission within minutes upon activity perturbation. This is achieved through homeostatic control of presynaptic exocytosis. We show that synergistic modulation of distinct presynaptic mechanisms not only maintains synaptic efficacy on rapid, but also on prolonged time scales. Homeostatic control of presynaptic exocytosis may be a general mechanism for stabilizing CNS function.

Abstract

Animal behavior is remarkably robust despite constant changes in neural activity. Homeostatic plasticity stabilizes central nervous system (CNS) function on time scales of hours to days. If and how CNS function is stabilized on more rapid time scales remains unknown. Here, we discovered that mossy fiber synapses in the mouse cerebellum homeostatically control synaptic efficacy within minutes after pharmacological glutamate receptor impairment. This rapid form of homeostatic plasticity is expressed presynaptically. We show that modulations of readily releasable vesicle pool size and release probability normalize synaptic strength in a hierarchical fashion upon acute pharmacological and prolonged genetic receptor perturbation. Presynaptic membrane capacitance measurements directly demonstrate regulation of vesicle pool size upon receptor impairment. Moreover, presynaptic voltage-clamp analysis revealed increased Ca ²⁺-current density under specific experimental conditions. Thus, homeostatic modulation of presynaptic exocytosis through specific mechanisms stabilizes synaptic transmission in a CNS circuit on time scales ranging from minutes to months. Rapid presynaptic homeostatic plasticity may ensure stable neural circuit function in light of rapid activity-dependent plasticity.