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      Additivity of Ileal Amino Acid Digestibility in Diets Containing Corn, Soybean Meal, and Corn Distillers Dried Grains with Solubles for Male Broilers

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          There is limited information on the additivity on ileal digestibility of amino acids (AAs) in poultry diets containing corn distillers dried grains with solubles. The aim of this study was to test additivity of two types of ileal digestibility of AAs in mixed diets containing corn, soybean meal, and corn distillers dried grains with solubles in male Ross 308 broiler chickens. The apparent ileal digestibility of some amino acids in feed ingredients used in the study was not additive in mixed diets, whereas the standardized ileal digestibility of most amino acids was additive. Based on the results, standardized ileal digestibility values are more additive in poultry diets containing various feed ingredients compared with apparent ileal digestibility values.

          Abstract

          The aim was to test additivity of apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AAs) in mixed diets for Ross 308 broiler chickens. Two hundred and eighty-eight, 20-d-old male broiler chickens were assigned to one of six diets, with six birds per cage using a randomized complete block design. The diets consisted of a nitrogen-free diet, three diets containing corn, soybean meal (SBM), and corn distillers dried grains with solubles (CDDGS) as the sole source of nitrogen, respectively, and two mixed diets containing corn, SBM or CDDGS. Chromic oxide was added to the diets as an indigestible index. On day 24, birds were euthanized for ileal digesta collection. Relative proportion of the basal endogenous loss of AAs to total ileal outflow of AAs in corn was greater ( p < 0.05) than that of SBM and CDDGS. For the corn-SBM and corn-SBM-CDDGS mixed diets, the AID of AAs differed ( p < 0.05) from the predicted values. No difference was observed between the measured and predicted SID of AA. In conclusion, the SID of AAs is more additive in mixed diets containing corn, SBM, or CDDGS compared to AID values.

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          AN IMPROVED PROCEDURE FOR THE DETERMINATION OF CHROMIC OXIDE IN FEED AND FECES

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            Evaluation of Amino Acid and Energy Utilization in Feedstuff for Swine and Poultry Diets

            An accurate feed formulation is essential for optimizing feed efficiency and minimizing feed cost for swine and poultry production. Because energy and amino acid (AA) account for the major cost of swine and poultry diets, a precise determination of the availability of energy and AA in feedstuffs is essential for accurate diet formulations. Therefore, the methodology for determining the availability of energy and AA should be carefully selected. The total collection and index methods are 2 major procedures for estimating the availability of energy and AA in feedstuffs for swine and poultry diets. The total collection method is based on the laborious production of quantitative records of feed intake and output, whereas the index method can avoid the laborious work, but greatly relies on accurate chemical analysis of index compound. The direct method, in which the test feedstuff in a diet is the sole source of the component of interest, is widely used to determine the digestibility of nutritional components in feedstuffs. In some cases, however, it may be necessary to formulate a basal diet and a test diet in which a portion of the basal diet is replaced by the feed ingredient to be tested because of poor palatability and low level of the interested component in the test ingredients. For the digestibility of AA, due to the confounding effect on AA composition of protein in feces by microorganisms in the hind gut, ileal digestibility rather than fecal digestibility has been preferred as the reliable method for estimating AA digestibility. Depending on the contribution of ileal endogenous AA losses in the ileal digestibility calculation, ileal digestibility estimates can be expressed as apparent, standardized, and true ileal digestibility, and are usually determined using the ileal cannulation method for pigs and the slaughter method for poultry. Among these digestibility estimates, the standardized ileal AA digestibility that corrects apparent ileal digestibility for basal endogenous AA losses, provides appropriate information for the formulation of swine and poultry diets. The total quantity of energy in feedstuffs can be partitioned into different components including gross energy (GE), digestible energy (DE), metabolizable energy (ME), and net energy based on the consideration of sequential energy losses during digestion and metabolism from GE in feeds. For swine, the total collection method is suggested for determining DE and ME in feedstuffs whereas for poultry the classical ME assay and the precision-fed method are applicable. Further investigation for the utilization of ME may be conducted by measuring either heat production or energy retention using indirect calorimetry or comparative slaughter method, respectively. This review provides information on the methodology used to determine accurate estimates of AA and energy availability for formulating swine and poultry diets.
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              Ileal digestibility of amino acids in feed ingredients for broilers

              To more precisely formulate feed and predict animal performance, it is important to base both the recommendations and feed formulations on digestible rather than total amino acid contents. Most published data on the digestibility of amino acids in feed ingredients for poultry are based on excreta digestibility. Ileal digestibility is an alternative and preferred approach to estimate amino acid availability in feed ingredients. Both methodologies are described and assessed. In addition, the differences between apparent and standardised (in which corrections are made for basal endogenous losses) digestible amino acid systems are discussed. The concept of a standardised digestibility system as a mean of overcoming the limitations of apparent digestibility estimates is proposed. In this context, different methodologies for the determination of basal endogenous amino acid losses are discussed. Although each methodology suffers from some limitations and published data on endogenous losses at the ileal level in growing poultry are limited, averaged data from repeated experiments using the ‘enzymatically hydrolysed casein’ method are considered as the best measure of basal losses. Standardised ileal amino acid digestibility values of 17 feed ingredients commonly used in broiler nutrition are presented including grains (barley, corn, sorghum, triticale, wheat), grain by-products (wheat middlings, rice pollard), plant protein sources (soybean meal, canola meal, corn gluten meal, cottonseed meal, lupins, peas/beans, sunflower meal), and animal by-products (feather meal, fish meal, meat and bone meal). This comprehensive set of the ileal amino acid digestibility of feed ingredients in broiler nutrition may serve as a basis for the establishment of the system in broiler feeding and for further research.
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                Author and article information

                Journal
                Animals (Basel)
                Animals (Basel)
                animals
                Animals : an Open Access Journal from MDPI
                MDPI
                2076-2615
                28 May 2020
                June 2020
                : 10
                : 6
                : 933
                Affiliations
                [1 ]Department of Animal Science, Kyungpook National University, Sangju 37224, Korea; woobi89@ 123456gmail.com
                [2 ]Monogastric Animal Feed Research Institute, Konkuk University, Seoul 05029, Korea; jungyeolsung@ 123456gmail.com
                [3 ]Institute of Poultry Science, National Institute of Animal Science, RDA, Pyeongchang 25340, Korea; magic100@ 123456korea.kr
                Author notes
                [* ]Correspondence: changsukong@ 123456knu.ac.kr ; Tel.: +82-54-530-1225
                Author information
                https://orcid.org/0000-0001-6236-6815
                https://orcid.org/0000-0002-3876-6488
                Article
                animals-10-00933
                10.3390/ani10060933
                7341220
                32481569
                989f0692-4089-4fe3-a0cf-38de72be58a4
                © 2020 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 28 April 2020
                : 25 May 2020
                Categories
                Article

                additivity,amino acids,ileal digestibility,feed ingredients,broiler

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