Genome plasticity favours double chromosomal Tn 4401b - bla KPC-2  transposon insertion in the  Pseudomonas aeruginosa  ST235 clone

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Abstract

Background

Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in bla _KPC-2 -positive P. aeruginosa ST235 in Colombia.

Results

In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the bla _VIM and bla _KPC-2 genes, respectively. The four bla _KPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn 6162-like with ant(2″)-Ia, two Tn 402-like with ant(3″)-Ia and bla _OXA-2 and two Tn 4401b with bla _KPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn 4401b copies harbouring bla _KPC-2 were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background.

Conclusion

This is the first report of a double Tn 4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism .

Electronic supplementary material

The online version of this article (10.1186/s12866-019-1418-6) contains supplementary material, which is available to authorized users.

Related collections

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Author and article information

Contributors

Deisy Abril: djabril@unbosque.edu.co

Ricaurte Alejandro Marquez-Ortiz: ramarquezo@gmail.com

Betsy Castro-Cardozo: betsy2402@gmail.com

José Ignacio Moncayo-Ortiz: jimo@utp.edu.co

Narda María Olarte Escobar: htunalcove@yahoo.com

Zayda Lorena Corredor Rozo: lore_rozo@yahoo.com

Niradiz Reyes: niradiz@gmail.com

Catalina Tovar: catalinatovar@unisinu.edu.co

Héctor Fabio Sánchez: chessanchez@gmail.com

Jaime Castellanos: castellanosjaime@unbosque.edu.co

Yina Marcela Guaca-González: yimagugo@utp.edu.co

Carmen Elisa Llanos-Uribe: coordinacionlaboratoriohusj@gmail.com

Natasha Vanegas Gómez: natashavanegas@yahoo.es

Javier Escobar-Pérez:

ORCID: http://orcid.org/0000-0002-0432-6978

57 1 6489000 , escobarjavier@unbosque.edu.co , javiesco21@yahoo.com

Journal

Journal ID (nlm-ta): BMC Microbiol

Journal ID (iso-abbrev): BMC Microbiol

Title: BMC Microbiology

Publisher: BioMed Central (London )

ISSN (Electronic): 1471-2180

Publication date (Electronic): 20 February 2019

Publication date PMC-release: 20 February 2019

Publication date Collection: 2019

Volume: 19

Electronic Location Identifier: 45

Affiliations

[1 ]ISNI 0000 0004 1761 4447, GRID grid.412195.a, Bacterial Molecular Genetics Laboratory, , Universidad El Bosque, ; Carrera 9 N°131A-02, Bogotá D.C, Colombia

[2 ]ISNI 0000 0001 2176 1069, GRID grid.412256.6, Grupo de Investigación en Enfermedades Infecciosas- GRIENI, Facultad de Ciencias de la Salud, , Universidad Tecnológica de Pereira, ; Pereira, Colombia

[3 ]Hospital El Tunal E.S.E, Bogotá D.C, Colombia

[4 ]ISNI 0000 0004 0486 624X, GRID grid.412885.2, Grupo de Genética y Biología Molecular, , Universidad de Cartagena, ; Cartagena, Colombia

[5 ]GRID grid.441931.a, Grupo de Investigación en Enfermedades Tropicales y Resistencia Bacteriana, , Universidad del Sinú, ; Montería, Colombia

[6 ]Hospital Universitario Departamental de Nariño, Pasto, Colombia

[7 ]ISNI 0000 0001 0286 3748, GRID grid.10689.36, Grupo de Patogénesis Infecciosa, , Universidad Nacional de Colombia, ; Bogotá D.C, Colombia

[8 ]ESE-Hospital Universitario San Jorge, Pereira, Colombia

[9 ]ISNI 0000 0004 1936 7611, GRID grid.117476.2, The i3 institute, Faculty of Science University of Technology, ; Sydney, Australia

Author information

Javier Escobar-Pérez http://orcid.org/0000-0002-0432-6978

Article

Publisher ID: 1418

DOI: 10.1186/s12866-019-1418-6

PMC ID: 6381643

PubMed ID: 30786858

SO-VID: 989852fc-5027-42a7-aa2b-1b66c686ff9e

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 15 December 2017

Date accepted : 12 February 2019

Funding

Funded by: Departamento Administrativo de Ciencia, Tecnología e Innovación (CO)

Award ID: 1308-657-41107 and 1308-569-33463

Award ID: 1308-657-41107

Funded by: Universidad El Bosque

Award ID: PCI63-2014

Custom metadata

ScienceOpen disciplines: Microbiology & Virology

Keywords: blakpc-2,pseudomonas aeruginosa,carbapenems,resistance,colombia,st235

Data availability:

ScienceOpen disciplines: Microbiology & Virology

Keywords: blakpc-2, pseudomonas aeruginosa, carbapenems, resistance, colombia, st235