RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

To engineer trehalose metabolism in sugarcane ( Saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (TPSP), to increase trehalose biosynthesis and an RNAi transgene specific for trehalase, to abrogate trehalose catabolism. In RNAi-expressing lines trehalase expression was abrogated in many plants however no decrease in trehalase activity was observed. In TPSP lines trehalase activity was significantly higher. No events of co-integration of TPSP and RNAi transgenes were observed. We suggest trehalase activity is essential to mitigate embryonic lethal effects of trehalose metabolism and discuss the implications for engineering trehalose metabolism.

Related collections

Most cited references 29

Record: found
Abstract: found
Article: not found

Inhibition of SNF1-related protein kinase1 activity and regulation of metabolic pathways by trehalose-6-phosphate.

Henriette Schluepmann, Erick J. Paul, Astrid Wingler … (2009)

Trehalose-6-phosphate (T6P) is a proposed signaling molecule in plants, yet how it signals was not clear. Here, we provide evidence that T6P functions as an inhibitor of SNF1-related protein kinase1 (SnRK1; AKIN10/AKIN11) of the SNF1-related group of protein kinases. T6P, but not other sugars and sugar phosphates, inhibited SnRK1 in Arabidopsis (Arabidopsis thaliana) seedling extracts strongly (50%) at low concentrations (1-20 microM). Inhibition was noncompetitive with respect to ATP. In immunoprecipitation studies using antibodies to AKIN10 and AKIN11, SnRK1 catalytic activity and T6P inhibition were physically separable, with T6P inhibition of SnRK1 dependent on an intermediary factor. In subsequent analysis, T6P inhibited SnRK1 in extracts of all tissues analyzed except those of mature leaves, which did not contain the intermediary factor. To assess the impact of T6P inhibition of SnRK1 in vivo, gene expression was determined in seedlings expressing Escherichia coli otsA encoding T6P synthase to elevate T6P or otsB encoding T6P phosphatase to decrease T6P. SnRK1 target genes showed opposite regulation, consistent with the regulation of SnRK1 by T6P in vivo. Analysis of microarray data showed up-regulation by T6P of genes involved in biosynthetic reactions, such as genes for amino acid, protein, and nucleotide synthesis, the tricarboxylic acid cycle, and mitochondrial electron transport, which are normally down-regulated by SnRK1. In contrast, genes involved in photosynthesis and degradation processes, which are normally up-regulated by SnRK1, were down-regulated by T6P. These experiments provide strong evidence that T6P inhibits SnRK1 to activate biosynthetic processes in growing tissues.

0 comments Cited 198 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Sugar signals and molecular networks controlling plant growth.

Sjef Smeekens, Jingkun Ma, Johannes Hanson … (2010)

In recent years, several regulatory systems that link carbon nutrient status to plant growth and development have emerged. In this paper, we discuss the growth promoting functions of the hexokinase (HXK) glucose sensor, the trehalose 6-phosphate (T6P) signal and the Target of Rapamycin (TOR) kinase pathway, and the growth inhibitory function of the SNF1-related Protein Kinase1 (SnRK1) and the C/S1 bZIP transcription factor network. It is crucial that these systems interact closely in regulating growth and in several cases crosstalk has been demonstrated. Importantly, these nutrient controlled systems must interact with other growth regulatory pathways. Copyright 2009 Elsevier Ltd. All rights reserved.

0 comments Cited 178 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Trehalose and plant stress responses: friend or foe?

Olivier Fernandez, Linda Béthencourt, Anthony Quéro … (2010)

The disaccharide trehalose is involved in stress response in many organisms. However, in plants, its precise role remains unclear, although some data indicate that trehalose has a protective role during abiotic stresses. By contrast, some trehalose metabolism mutants exhibit growth aberrations, revealing potential negative effects on plant physiology. Contradictory effects also appear under biotic stress conditions. Specifically, trehalose is essential for the infectivity of several pathogens but at the same time elicits plant defense. Here, we argue that trehalose should not be regarded only as a protective sugar but rather like a double-faced molecule and that further investigation is required to elucidate its exact role in stress tolerance in plants.

0 comments Cited 133 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

: brian.oneill@uqconnect.edu.au

: matthewpurnell@gmail.com

: Lars.Nielsen@uq.edu.au

: sbrumbley@unt.edu

Journal

Journal ID (nlm-ta): Springerplus

Journal ID (iso-abbrev): Springerplus

Title: SpringerPlus

Publisher: Springer International Publishing AG (Cham )

ISSN (Electronic): 2193-1801

Publication date (Electronic): 21 December 2012

Publication date PMC-release: 21 December 2012

Publication date Collection: 2012

Volume: 1

Issue: 1

Electronic Location Identifier: 74

Affiliations

[1 ]Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland 4072 Australia

[2 ]BSES Limited, PO Box 86, Indooroopilly, Queensland 4068 Australia

[3 ]Department of Biological Sciences, University of North Texas, 1155 Union Circle #305220, Denton, TX 76203-5017 USA

Article

Publisher ID: 160

DOI: 10.1186/2193-1801-1-74

PMC ID: 3606522

PubMed ID: 23539210

SO-VID: 984c4dbd-2bf9-46b3-99bb-a879b61cd755

License:

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

History

Date received : 7 September 2012

Date accepted : 1 December 2012

Custom metadata

ScienceOpen disciplines: Uncategorized

Keywords: rna-interference,saccharum,sucrose-derivatives,sugarcane biofactory,trehalase, trehalose

Data availability:

ScienceOpen disciplines: Uncategorized

Keywords: rna-interference, saccharum, sucrose-derivatives, sugarcane biofactory, trehalase, trehalose

Comments

Comment on this article

scite_

Smart Citations

Citing PublicationsSupportingMentioningContrasting

View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.