For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
0
views
11
references
 Top references
cited by
0
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      215
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: not found

      Analysis of bovine parainfluenza-3 virus replication in bovine embryonic lung cells by indirect fluorescent antibody and hemadsorption assays

      brief-report

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The temporal manifestation of bovine parainfluenza-3 virus (BPI3V) proteins in the cytoplasm and on the surface of bovine embryonic lung (BEL) cells was characterized by indirect fluorescent antibody (IFA) and hemadsorption (HAd) assays. Intracellular proteins appeared earliest at 0.5 h post inoculation (p.i.) and the infection spread to virtually all cells by 48 h p.i. Viral proteins on the surface of cells were seen first at 16 h p.i., and by 48 h p.i. the entire cell monolayer was IFA positive. Hundred-fold less virus in the inoculum delayed appearance of the intracellular as well as cell-surface viral proteins by 24 h and allowed the infection of only about 13 of the cells by 48 h p.i. Kinetics of the development in the proportion of HAd-positive cells correlated with those of the surface fluorescence-positive cells. Morphology of the manifestation of BPI3V proteins is characterized by microphotography.

          Related collections

          Most cited references11

          • Record: found
          • Abstract: found
          • Article: not found

          Trypsin-enhanced replication of neonatal calf diarrhea coronavirus in bovine embryonic lung cells.

          T Toth (1982)
          Bovine embryonic lung (BEL) cells, grown in 8-chamber cell-culture slides, were inoculated with 2.5, 25, and 250 TCID50 of neonatal calf diarrhea coronavirus (NCDCV)/chamber. The NCDCV inocula were prepared in serum-free, trypsin-free regular medium (RM), and in serum-free medium containing 1 microgram (TM1), 5 microgram (TM5), and 10 microgram (TM10) of trypsin/ml; the the cells were maintained under identical media. Cytopathic effect (CPE), hemadsorption of rat RBC, formation of syncytia, appearance of immunofluorescing cells, and release of infectious virus was followed from postinoculation hours 4 to 100. Maintaining the BEL cells in medium containing trypsin up to 10 microgram/ml did not affect them adversely. The BEL cells appeared resistant to infection with NCDCV in RM by CPE, syncytia, immunofluorescing cells, or infectious virus-release parameters. In TM1, TM5, and TM10, all parameters were positive for NCDCV replication. They manifested and developed in positive correlation with increasing trypsin concentration from 1 microgram to 10 microgram/ml. Release of infectious virions preceded all other parameters of virus replication. Replication of NCDCV was demonstrated earlier by hemadsorption and immunofluorescing cells than by syncytia or CPE. The TM5 and TM10 increased the susceptibility of BEL cells to NCDCV by more than a millionfold, as measured by the titer of released virus in these media (greater than 1-(6) TCID50/ml) as compared with those in RM (infectious virus not demonstrated).
          Article 0 comments Cited 14 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Aerosol stability of bovine parainfluenza type 3 virus.

            M Elazhary, J Derbyshire (1979)
            Aerosols of bovine parainfluenza type 3 virus were generated with a Devilbiss 40 nebulizer from Eagle's minimum essential medium and nasal secretion from a non-infected calf and stored in a rotating drum at temperatures of 6 degrees C or 32 degrees C and relative humidities of 30% or 90%. The aerosols were sampled at seven minutes, one, two and three hours after the start of generation with an all glass impinger (AGI-30) and titrated for infectivity in cell cultures. Physical decay was determined by a rhodamine tracer technique. Media, temperature or relative humidity had little effect on the survival of parainfluenza type 3 virus during spraying (zero to seven minutes). During aging of aerosols at 32 degrees C and 30% relative humidity, parainfluenza type 3 virus was less stable in Eagle's minimum essential medium than in nasal secretion from a noninfected calf, but at 6 degrees C and 30% relative humidity, the virus was more stable in Eagle's minimum essential medium. At 32 degrees C, the virus was less stable during aging at 90% relative humidity than at 30% relative humidity. The virus was consistently more stable during aging of aerosols at 6 degrees C than at 32 degrees C.
            Article 0 comments Cited 8 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Bovine parainfluenza type 3 virus infection: virus replication in bovine embryonic cell cultures and virion separation by rate-zonal centrifugation.

              K. Tsai, R G Thomson (1975)
              Replicative sequences of a bovine strain of parainfluenza type 3 virus in bovine embryonic kidney and spleen cell cultures were investigated by light and fluorescence microscopy and by ultrathin section and negative-contrast electron microscopy. Observations from light and fluorescence microscopy showed that intracytoplasmic inclusions were detected as small granules surrounding the nuclei of more than 90 percent of the cell population by day 2 postinoculation. With the increase of postexposure times, these inclusions coalesced into larger bodies which occupied large portions of the cell. Ultrastructurally, the first sign of virus development was the appearance of aggregates of viral nucleocapsids in the vicinity of the nucleus. With the concomitant accumulation of viral nucleocapsids in the cytoplasm, the virus maturation was expressed by budding processes through the cell membrane into round, oval, or elongated forms. Eosinophilic inclusions were demonstrable in many mitotic cells. Ultrastructurally, these cells were observed to produce virus particles by a process identical to that of resting cells. Virions, prepared from infected culture fluid and negatively stained, appeared to be pleomorphic and their diameter ranged from 200 to 600 mm. The virions were separated, by rate-zonal centrifugation, into two subclasses in a sucrose gradient (15 to 60 percent, wt/wt). The slowly sedimenting virions had a density approximately 1.20 gm/cm3 and an average size of 200 nm in diameter, whereas the faster-sedimenting virions had a density of 1.24 gm/cm3 and average diameter of 400 nm.
              Article 0 comments Cited 2 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): J Virol Methods
                Journal ID (iso-abbrev): J. Virol. Methods
                Title: Journal of Virological Methods
                Publisher: Published by Elsevier B.V.
                ISSN (Print): 0166-0934
                ISSN (Electronic): 1879-0984
                Publication date PMC-release: 12 November 2002
                Publication date (Print): January 1990
                Publication date (Electronic): 12 November 2002
                Volume: 27
                Issue: 1
                Pages: 113-119
                Affiliations
                Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061, U.S.A.
                Author notes
                [∗ ]Correspondence to: T.E. Toth, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061, U.S.A..
                Article
                Publisher ID: 0166-0934(90)90151-5
                DOI: 10.1016/0166-0934(90)90151-5
                PMC ID: 7119780
                PubMed ID: 2155247
                SO-VID: 957a16f5-c531-4598-9d77-a4157f9b2f02
                Copyright © Copyright © 1990 Published by Elsevier B.V.
                License:

                Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

                History
                Date accepted : 5 September 1989
                Categories
                Subject: Article

                ScienceOpen disciplines: Microbiology & Virology
                Keywords: bovine parainfluenza-3 virus,bovine embryonic lung,indirect fluorescent antibody,hemadsorption
                Data availability:
                ScienceOpen disciplines: Microbiology & Virology
                Keywords: bovine parainfluenza-3 virus, bovine embryonic lung, indirect fluorescent antibody, hemadsorption

                Comments

                Comment on this article