„Natural” killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype
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Abstract
In the spleens of young, adult mice there exist naturally occurring killer lymphocytes
with specificity for mouse Moloney leukemia cells. The lytic activity was directed
against syngeneic or allogeneic Moloney leukemia cells to a similar extent, but was
primarily expressed when tested against in vitro grown leukemia cells. Two leukemias
of non-Moloney origin were resistant and so was the mastocytoma line P815. Although
killer activity varied between different strains of mice, the specificity of lysis
was the same as indicated by competition experiments using unlabeled Moloney or other
tumor cells as inhibitors in the cytotoxic assays. Capacity to compete and sensitivy
to lysis by the killer cells were found to be highly positively correlated. Analysis
of the kinetics of the cytotoxic assay revealed a rapid induction of lysis within
one to four hours, arguing against any conventional in vitro induction of immune response.
No evidence was found of soluble factors playing any role in the cytolytic assay.