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      Draft Genome Sequences of Bacteroides pyogenes Strains Isolated from the Uterus of Holstein Dairy Cows with Metritis

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          Abstract

          Bacteroides pyogenes is found in the human and animal gut and is implicated in the pathogenesis of metritis in cows. We report the draft genome sequences of four Bacteroides pyogenes isolates obtained from the uterus of metritic cows. This will increase the understanding of its pathogenicity, antimicrobial resistance, and differentiation across hosts.

          ABSTRACT

          Bacteroides pyogenes is found in the human and animal gut and is implicated in the pathogenesis of metritis in cows. We report the draft genome sequences of four Bacteroides pyogenes isolates obtained from the uterus of metritic cows. This will increase the understanding of its pathogenicity, antimicrobial resistance, and differentiation across hosts.

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          Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows.

          Rodolfo D Mingoti, Klibs N. Galvão, Rodolfo Daetz … (2015)
          The objective of this study was to evaluate the progression of the uterine microbiota from calving until establishment of metritis. Uterine swabs (n = 72) collected at 0, 2, and 6 ± 2 days postpartum (dpp) from 12 metritic and 12 healthy cows were used for metagenomic sequencing of the 16S rRNA gene on the Illumina MiSeq platform. A heat map showed that uterine microbiota was established at calving. The microbiota changed rapidly from 0 to 6 ± 2 dpp, with a decrease in the abundance of Proteobacteria and an increase in the abundance of Bacteroidetes and Fusobacteria, which were dominant in metritic cows. Uterine microbiota composition was shared; however, metritic and healthy cows could be discriminated using relative abundance of bacterial genera at 0, 2, and 6 ± 2 dpp. Bacteroides was the main genus associated with metritis because it was the only genus that showed significantly greater abundance in cows with metritis. As the abundance of Bacteroides organisms increased, the uterine discharge score, a measure of uterine health, worsened. Fusobacterium was also an important genus associated with metritis because Fusobacterium abundance increased as Bacteroides abundance increased and the uterine discharge score worsened as the abundance increased. The correlation with uterine discharge score and the correlation with Bacteroides or Fusobacterium showed that other bacteria, such as Helcoccocus, Filifactor, and Porphyromonas, were also associated with metritis. There were also bacteria associated with uterine health, such as "Candidatus Blochmannia," Escherichia, Sneathia, and Pedobacter.
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            Quantifying known and emerging uterine pathogens, and evaluating their association with metritis and fever in dairy cows

            K. Jeong, Klibs N. Galvão, Jimena Laporta … (2018)
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              Distribution and mobility of the tetracycline resistance determinant tetQ.

              Elise Riley, J. L. Krieger, Z. Leng … (1997)
              We tested 34 American Type Culture Collection (ATCC) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetQ gene using a polymerase chain reaction (PCR) assay and DNA-DNA hybridization. The identities of PCR products were confirmed by Southern blot hybridization of whole-cell DNA. Eleven of the ATCC strains were positive for tetQ, including five Bacteroides spp., five Prevotella spp. and a single isolate of Mitsuokella multiacidus. Twenty-eight (29%) of the 95 clinical Gram-negative isolates carried the tetQ gene, while eight (11%) of the 73 clinical Gram-positive isolates carried the tetQ gene. This is the first description of tetQ in Gram-positive species. All isolates except one Peptostreptococcus sp. carried tetQ integrated into the chromosome. The tetQ gene could be transferred from Prevotella bivia, Bacteroides ovatus, Bacteroides fragilis, Bacteroides vulgatus and Bacteroides distasonis into an Enterococcus faecalis recipient at frequencies of 10(-7)-10(-9) per recipient. In contrast, tetQ failed to transfer from two isolates of Prevotella intermedia, two isolates of Porphyromonas gingivalis, one isolate of Mobiluncus curtisii and one isolate of Peptostreptococcus sp. The latter two are Gram-positive species. The PCR assay was used to screen 198 proteinase K-treated biopsies of prostate, periprostate and bladder from 84 men with prostatitis. Thirty-four (40%) of the patients had one or more positive samples, suggesting that the PCR assay could be of value in screening patient material directly for the presence of bacteria.
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                Author and article information

                Contributors
                David A. Baltrus: Role: Editor
                Journal
                Journal ID (nlm-ta): Microbiol Resour Announc
                Journal ID (iso-abbrev): Microbiol Resour Announc
                Journal ID (hwp): ga
                Journal ID (pmc): mra
                Journal ID (publisher-id): MRA
                Title: Microbiology Resource Announcements
                Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )
                ISSN (Electronic): 2576-098X
                Publication date (Electronic): 10 October 2019
                Publication date Collection: October 2019
                Volume: 8
                Issue: 41
                Electronic Location Identifier: e01043-19
                Affiliations
                [a ]Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA
                [b ]Department of Animal Sciences, University of Florida, Gainesville, Florida, USA
                [c ]Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA
                [d ]D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, Florida, USA
                University of Arizona
                Author notes
                Address correspondence to Klibs N. Galvão, galvaok@ 123456ufl.edu .
                [*]

                Present address: Soo Jin Jeon, College of Veterinary Medicine, Long Island University, Brookville, New York, USA.

                Citation Cunha F, Jeon SJ, Jeong KC, Galvão KN. 2019. Draft genome sequences of Bacteroides pyogenes strains isolated from the uterus of Holstein dairy cows with metritis. Microbiol Resour Announc 8:e01043-19. https://doi.org/10.1128/MRA.01043-19.

                Article
                Publisher ID: MRA01043-19
                DOI: 10.1128/MRA.01043-19
                PMC ID: 6787326
                PubMed ID: 31601669
                SO-VID: 908c0b6c-4a4b-47ea-85fd-f773cf1b21f9
                Copyright © Copyright © 2019 Cunha et al.
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

                History
                Date received : 28 August 2019
                Date accepted : 18 September 2019
                Page count
                Figures: 0, Tables: 1, Equations: 0, References: 16, Pages: 3, Words: 1646
                Funding
                Funded by: U.S. Department of Agriculture (USDA), https://doi.org/10.13039/100000199;
                Award ID: 1002880
                Award Recipient :
                Categories
                Subject: Genome Sequences
                Custom metadata
                cover-date October 2019

                Data availability:

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