In vivo bioimaging and detection of endogenous hypochlorous acid in lysosome using a near-infrared fluorescent probe.

Abstract

The phagocyte's lysosome is the primary site of hypochlorous acid (HOCl) synthesis, and HOCl can be used as a biomarker for osteoarthritis diagnosis and treatment evaluation. Accurate detection of HOCl with high sensitivity and selectivity is required to understand its activities in healthy bio-systems and diseases. By integrating acceptable design principles and dye screening methodologies, we proposed and developed a novel near-infrared fluorescent HOCl sensing probe (FNIR-HOCl). The FNIR-HOCl probe has a quick reaction rate, high sensitivity (LOD = 70 nM), and excellent selectivity toward HOCl over other metal ions and reactive oxygen species. It has been successfully implemented to detect endogenous HOCl produced by RAW264.7 cells, as well as in vivo imaging towards mice with osteoarthritis. As a result, the probe FNIR-HOCl is extremely promising as a biological tool for revealing the roles of HOCl in various physiological and pathological contexts.