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      Dataset from de novo transcriptome assembly of Myristica fatua leaves using MinION nanopore sequencer

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          Abstract

          Myristica fatua is a tropical fruit tree species originating from Indonesia. Very few genomic resources are available for the species. We developed a full-length transcriptome assembly using long-read sequencing (MinION Nanopore technology) and produced 4.3 million reads (3.5 G of bases). The assembled full-length transcript was constructed using the RATTLE program and assembled 21,098 transcripts. The transcript ranged from 201 – 14,174 bp, and N50 was 2,017 bp. The transcripts were annotated with the UNIPROT database using BlastX. The functional annotation was performed using Blast2go software. The 8,445 microsatellite motif-containing contigs were identified. The raw reads are deposited in the ENA (European Nucleotide Archive) with ENA experiment accession number ERX6798613.

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          Most cited references17

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          Basic local alignment search tool.

          A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score. Recent mathematical results on the stochastic properties of MSP scores allow an analysis of the performance of this method as well as the statistical significance of alignments it generates. The basic algorithm is simple and robust; it can be implemented in a number of ways and applied in a variety of contexts including straightforward DNA and protein sequence database searches, motif searches, gene identification searches, and in the analysis of multiple regions of similarity in long DNA sequences. In addition to its flexibility and tractability to mathematical analysis, BLAST is an order of magnitude faster than existing sequence comparison tools of comparable sensitivity.
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            Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research.

            We present here Blast2GO (B2G), a research tool designed with the main purpose of enabling Gene Ontology (GO) based data mining on sequence data for which no GO annotation is yet available. B2G joints in one application GO annotation based on similarity searches with statistical analysis and highlighted visualization on directed acyclic graphs. This tool offers a suitable platform for functional genomics research in non-model species. B2G is an intuitive and interactive desktop application that allows monitoring and comprehension of the whole annotation and analysis process. Blast2GO is freely available via Java Web Start at http://www.blast2go.de. http://www.blast2go.de -> Evaluation.
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              NanoPack: visualizing and processing long-read sequencing data

              Abstract Summary Here we describe NanoPack, a set of tools developed for visualization and processing of long-read sequencing data from Oxford Nanopore Technologies and Pacific Biosciences. Availability and implementation The NanoPack tools are written in Python3 and released under the GNU GPL3.0 License. The source code can be found at https://github.com/wdecoster/nanopack, together with links to separate scripts and their documentation. The scripts are compatible with Linux, Mac OS and the MS Windows 10 subsystem for Linux and are available as a graphical user interface, a web service at http://nanoplot.bioinf.be and command line tools. Supplementary information Supplementary data are available at Bioinformatics online.
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                Author and article information

                Contributors
                @matradeden
                Journal
                Data Brief
                Data Brief
                Data in Brief
                Elsevier
                2352-3409
                17 December 2022
                February 2023
                17 December 2022
                : 46
                : 108838
                Affiliations
                [a ]Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia
                [b ]Agronomy and Horticulture Study Program, Graduate School of IPB University, Bogor, Indonesia
                [c ]Agrotechnology Study Program, Faculty of Agriculture, Nusa Bangsa University, Bogor, Indonesia
                [d ]Department of Plant Science, Politeknik Negeri Lampung, Lampung, Indonesia
                [e ]DIADE, IRD, Univ Montpellier, CIRAD, Montpellier, France
                Author notes
                Article
                S2352-3409(22)01041-1 108838
                10.1016/j.dib.2022.108838
                9803912
                36593766
                8c5bdb9b-d5a1-491f-81ef-c72603b0e07a
                © 2022 The Author(s)

                This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

                History
                : 21 September 2022
                : 7 December 2022
                : 12 December 2022
                Categories
                Data Article

                conservation,genetic diversity,long-reads sequencing,spices,full-length transcript

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