Propagation of electrical activity in uterine muscle during pregnancy: a review

On the basis of a literature review, this work summarizes uterine animal and human electromyographic information obtained at cellular, myometrial, and abdominal levels during gestation and parturition. We show that both internal and external electromyograms occur in phase with intrauterine pressure increase and exhibit similar spectra, including a slow wave (0.01 < frequency < 0.03 Hz) probably because of mechanical artifacts and a fast wave whose frequency content can be subdivided into a low-frequency band always present in every contraction and a high-frequency band related to efficient parturition contractions. Application of classic spectral techniques to electromyogram envelopes has identified group propagation but not pacemaker areas. However, no time delay or classic propagation has been demonstrated by applying the same spectral techniques to the electromyogram itself, probably because of the nonlinearity and three-dimensional nature of the propagating process.

Power spectrum (PS) of uterine electromyography (EMG) can identify true labor. EMG propagation velocity (PV) to diagnose labor has not been reported. The objective was to compare uterine EMG against current methods to predict preterm delivery. EMG was recorded in 116 patients (preterm labor, n = 20; preterm nonlabor, n = 68; term labor, n = 22; term nonlabor, n = 6). A Student t test was used to compare EMG values for labor vs nonlabor (P < .05, significant). Predictive values of EMG, Bishop score, contractions on tocogram, and transvaginal cervical length were calculated using receiver-operator characteristics analysis. PV was higher in preterm and term labor compared with nonlabor (P < .001). Combined PV and PS peak frequency predicted preterm delivery within 7 days with area under the curve (AUC) of 0.96. Bishop score, contractions, and cervical length had an AUC of 0.72, 0.67, and 0.54. Uterine EMG PV and PS peak frequency more accurately identify true preterm labor than clinical methods. Copyright © 2011 Mosby, Inc. All rights reserved.

The mechanism underlying spontaneous pacemaker potential in the uterus is not clearly understood. Several spontaneously active smooth muscles have interstitial cells of Cajal (ICCs) or ICC-like cells. We therefore examined cells from freshly dispersed uterine muscle strips (from pregnant human and rat myometrium) and in situ uterine preparations to determine the cell types present. Both preparations revealed numerous ICC-like cells; they were multipolar, with spider-like projections and enlarged central regions. These cells were readily distinguished from uterine myocytes by their morphology and ultrastructure, i.e., no myofilaments, numerous mitochondria, caveolae, and filaments. In addition, the ICC-like cells were noncontractile. These cells were negative to c-kit, a classic marker for ICCs. They stained positive for the intermediate filament, vimentin, a marker for cells of mesenchymal origin but not differentiated myocytes. The ICC-like cells had a more or less stable resting membrane potential of -58+/-7 mV compared with smooth-muscle cells, -65+/-13 mV, and produced outward current in response to voltage clamp pulses. However, in contrast with uterine myocytes, inward currents were not observed. This is the first description of ICC-like cells in myometrium and their role in the uterus is discussed, as possible inhibitors of intrinsic smooth-muscle activity.