Chikungunya virus transmission between Aedes albopictus and laboratory mice

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus associated with epidemics of acute and chronic arthritic disease in humans. Aedes albopictus has emerged as an important new natural vector for CHIKV transmission; however, mouse models for studying transmission have not been developed.

Methods

Aedes albopictus mosquitoes were infected with CHIKV via membrane feeding and by using infected adult wild-type C57BL/6 mice. Paraffin sections of infected mosquitoes were analysed by immunofluorescent antibody staining using an anti-CHIKV antibody. CHIKV-infected mosquitoes were used to infect adult C57BL/6 and interferon response factor 3 and 7 deficient (IRF3/7 ^-/-) mice.

Results

Feeding mosquitoes on blood meals with CHIKV titres > 5 log ₁₀CCID ₅₀/ml, either by membrane feeding or feeding on infected mice, resulted in ≥ 50 % of mosquitoes becoming infected. However, CHIKV titres in blood meals ≥ 7 log ₁₀CCID ₅₀/ml were required before salivary glands showed significant levels of immunofluorescent staining with an anti-CHIKV antibody. Mosquitoes fed on blood meals of 7.5 (but not 5.9) log ₁₀CCID ₅₀/ml were able efficiently to transmit virus to adult C57BL/6 and IRF3/7 ^-/- mice, with the latter mice showing overt signs of arthritis post-infection.

Conclusions

The results provide a simple in vivo model for studying transmission of CHIKV from mosquitoes to mammals and also argue against a resistance barrier to CHIKV infection in adult mice.

Electronic supplementary material

The online version of this article (doi:10.1186/s13071-016-1838-1) contains supplementary material, which is available to authorized users.

Related collections

Most cited references 30

Record: found
Abstract: found
Article: not found

Innate immunity of the newborn: basic mechanisms and clinical correlates.

Ofer Levy (2007)

The fetus and newborn face a complex set of immunological demands, including protection against infection, avoidance of harmful inflammatory immune responses that can lead to pre-term delivery, and balancing the transition from a sterile intra-uterine environment to a world that is rich in foreign antigens. These demands shape a distinct neonatal innate immune system that is biased against the production of pro-inflammatory cytokines. This bias renders newborns at risk of infection and impairs responses to many vaccines. This Review describes innate immunity in newborns and discusses how this knowledge might be used to prevent and treat infection in this vulnerable population.

0 comments Cited 349 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Two Chikungunya Isolates from the Outbreak of La Reunion (Indian Ocean) Exhibit Different Patterns of Infection in the Mosquito, Aedes albopictus

Marie Vazeille, Sara Moutailler, Daniel Coudrier … (2007)

Background A Chikungunya (CHIK) outbreak hit La Réunion Island in 2005–2006. The implicated vector was Aedes albopictus. Here, we present the first study on the susceptibility of Ae. albopictus populations to sympatric CHIKV isolates from La Réunion Island and compare it to other virus/vector combinations. Methodology and Findings We orally infected 8 Ae. albopictus collections from La Réunion and 3 from Mayotte collected in March 2006 with two Chikungunya virus (CHIKV) from La Réunion: (i) strain 05.115 collected in June 2005 with an Alanine at the position 226 of the glycoprotein E1 and (ii) strain 06.21 collected in November 2005 with a substitution A226V. Two other CHIKV isolates and four additional mosquito strains/species were also tested. The viral titer of the infectious blood-meal was 107 plaque forming units (pfu)/mL. Dissemination rates were assessed by immunofluorescent staining on head squashes of surviving females 14 days after infection. Rates were at least two times higher with CHIKV 06.21 compared to CHIKV 05.115. In addition, 10 individuals were analyzed every day by quantitative RT-PCR. Viral RNA was quantified on (i) whole females and (ii) midguts and salivary glands of infected females. When comparing profiles, CHIKV 06.21 produced nearly 2 log more viral RNA copies than CHIKV 05.115. Furthermore, females infected with CHIKV 05.115 could be divided in two categories: weakly susceptible or strongly susceptible, comparable to those infected by CHIKV 06.21. Histological analysis detected the presence of CHIKV in salivary glands two days after infection. In addition, Ae. albopictus from La Réunion was as efficient vector as Ae. aegypti and Ae. albopictus from Vietnam when infected with the CHIKV 06.21. Conclusions Our findings support the hypothesis that the CHIK outbreak in La Réunion Island was due to a highly competent vector Ae. albopictus which allowed an efficient replication and dissemination of CHIKV 06.21.

0 comments Cited 244 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: found

Is Open Access

Emergence and potential for spread of Chikungunya virus in Brazil

Marcio Roberto Teixeira Nunes, Nuno Rodrigues Faria, Janaina Mota de Vasconcelos … (2015)

Background In December 2013, an outbreak of Chikungunya virus (CHIKV) caused by the Asian genotype was notified in the Caribbean. The outbreak has since spread to 38 regions in the Americas. By September 2014, the first autochthonous CHIKV infections were confirmed in Oiapoque, North Brazil, and in Feira de Santana, Northeast Brazil. Methods We compiled epidemiological and clinical data on suspected CHIKV cases in Brazil and polymerase-chain-reaction-based diagnostic was conducted on 68 serum samples from patients with symptom onset between April and September 2014. Two imported and four autochthonous cases were selected for virus propagation, RNA isolation, full-length genome sequencing, and phylogenetic analysis. We then followed CDC/PAHO guidelines to estimate the risk of establishment of CHIKV in Brazilian municipalities. Results We detected 41 CHIKV importations and 27 autochthonous cases in Brazil. Epidemiological and phylogenetic analyses indicated local transmission of the Asian CHIKV genotype in Oiapoque. Unexpectedly, we also discovered that the ECSA genotype is circulating in Feira de Santana. The presumed index case of the ECSA genotype was an individual who had recently returned from Angola and developed symptoms in Feira de Santana. We estimate that, if CHIKV becomes established in Brazil, transmission could occur in 94% of municipalities in the country and provide maps of the risk of importation of each strain of CHIKV in Brazil. Conclusions The etiological strains associated with the early-phase CHIKV outbreaks in Brazil belong to the Asian and ECSA genotypes. Continued surveillance and vector mitigation strategies are needed to reduce the future public health impact of CHIKV in the Americas. Electronic supplementary material The online version of this article (doi:10.1186/s12916-015-0348-x) contains supplementary material, which is available to authorized users.

0 comments Cited 212 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Leon E. Hugo: Leon.Hugo@qimrberghofer.edu.au

Natalie A. Prow: Natalie.Prow@qimrberghofer.edu.au

Bing Tang: Bing.Tang@qimrberghofer.edu.au

Greg Devine: Greg.Devine@qimrberghofer.edu.au

Andreas Suhrbier: Andreas.Suhrbier@qimrberghofer.edu.au

Journal

Journal ID (nlm-ta): Parasit Vectors

Journal ID (iso-abbrev): Parasit Vectors

Title: Parasites & Vectors

Publisher: BioMed Central (London )

ISSN (Electronic): 1756-3305

Publication date (Electronic): 19 October 2016

Publication date PMC-release: 19 October 2016

Publication date Collection: 2016

Volume: 9

Electronic Location Identifier: 555

Affiliations

[1 ]Inflammation Biology, QIMR Berghofer Medical Research Institute, Brisbane, Queensland 4029 Australia

[2 ]Mosquito Control, QIMR Berghofer Medical Research Institute, Brisbane, Queensland 4029 Australia

Article

Publisher ID: 1838

DOI: 10.1186/s13071-016-1838-1

PMC ID: 5069946

PubMed ID: 27760560

SO-VID: 890a58af-8eb0-420e-8d78-bb7507bfdeaa

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 12 June 2016

Date accepted : 6 October 2016

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100000925, National Health and Medical Research Council;

Award ID: APP1078468

Award Recipient : Andreas Suhrbier

Funded by: Perpetual JT Wilson Fellow

Funded by: John and Elizabeth Hunter

Funded by: Ed Westaway, Royal Australian Air Force Association

Custom metadata

ScienceOpen disciplines: Parasitology

Keywords: chikungunya virus,aedes albopictus,mouse model

Data availability:

ScienceOpen disciplines: Parasitology

Keywords: chikungunya virus, aedes albopictus, mouse model

Comments

Comment on this article

scite_

Cited by 15

See all cited by

Most referenced authors 455

See all reference authors

Chikungunya virus transmission between Aedes albopictus and laboratory mice

Read this article at

Abstract

Background

Methods

Results

Conclusions

Electronic supplementary material

Related collections

Decoding Infection and Transmission

Most cited references 30

Innate immunity of the newborn: basic mechanisms and clinical correlates.

Two Chikungunya Isolates from the Outbreak of La Reunion (Indian Ocean) Exhibit Different Patterns of Infection in the Mosquito, Aedes albopictus

Emergence and potential for spread of Chikungunya virus in Brazil

Author and article information

Contributors

Journal

Affiliations

Article

History

Funding

Categories

Custom metadata

Comments

Comment on this article

Similar content 1,159

Cited by 15

Most referenced authors 455