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      Current status of biotechnological production and applications of microbial exopolysaccharides

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          Abstract

          <p class="first" id="d5619657e73">Microbial exopolysaccharides (EPS) are an abundant and important group of compounds that can be secreted by bacteria, fungi and algae. The biotechnological production of these substances represents a faster alternative when compared to chemical and plant-derived production with the possibility of using industrial wastes as substrates, a feasible strategy after a comprehensive study of factors that may affect the synthesis by the chosen microorganism and desirable final product. Another possible difficulty could be the extraction and purification methods, a crucial part of the production of microbial polysaccharides, since different methods should be adopted. In this sense, this review aims to present the biotechnological production of microbial exopolysaccharides, exploring the production steps, optimization processes and current applications of these relevant bioproducts. </p>

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          Bacterial Extracellular Polysaccharides Involved in Biofilm Formation

          Extracellular polymeric substances (EPS) produced by microorganisms are a complex mixture of biopolymers primarily consisting of polysaccharides, as well as proteins, nucleic acids, lipids and humic substances. EPS make up the intercellular space of microbial aggregates and form the structure and architecture of the biofilm matrix. The key functions of EPS comprise the mediation of the initial attachment of cells to different substrata and protection against environmental stress and dehydration. The aim of this review is to present a summary of the current status of the research into the role of EPS in bacterial attachment followed by biofilm formation. The latter has a profound impact on an array of biomedical, biotechnology and industrial fields including pharmaceutical and surgical applications, food engineering, bioremediation and biohydrometallurgy. The diverse structural variations of EPS produced by bacteria of different taxonomic lineages, together with examples of biotechnological applications, are discussed. Finally, a range of novel techniques that can be used in studies involving biofilm-specific polysaccharides is discussed.
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            Hyaluronic acid based scaffolds for tissue engineering--a review.

            This review focuses on hyaluronic acid (HA) tissue scaffolding materials. Scaffolds are defined in terms of formation mechanisms and mode of action. Solution properties are discussed as an understanding of the hydrodynamics of HA is fundamental in optimising the subsequent modification and the chemistries behind important tissue engineering applications that are emerging from recent research on this increasingly valuable carbohydrate polymer are described. Key scaffold characteristics such as mechanical, biological function and degradation are discussed. The latest technologies behind scaffold processing are assessed and the applications of HA based scaffolds are discussed. Copyright © 2012 Elsevier Ltd. All rights reserved.
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              Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies

              Bacteria produce a wide range of exopolysaccharides which are synthesized via different biosynthesis pathways. The genes responsible for synthesis are often clustered within the genome of the respective production organism. A better understanding of the fundamental processes involved in exopolysaccharide biosynthesis and the regulation of these processes is critical toward genetic, metabolic and protein-engineering approaches to produce tailor-made polymers. These designer polymers will exhibit superior material properties targeting medical and industrial applications. Exploiting the natural design space for production of a variety of biopolymer will open up a range of new applications. Here, we summarize the key aspects of microbial exopolysaccharide biosynthesis and highlight the latest engineering approaches toward the production of tailor-made variants with the potential to be used as valuable renewable and high-performance products for medical and industrial applications.
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                Author and article information

                Journal
                Critical Reviews in Food Science and Nutrition
                Critical Reviews in Food Science and Nutrition
                Informa UK Limited
                1040-8398
                1549-7852
                May 14 2020
                February 11 2019
                May 14 2020
                : 60
                : 9
                : 1475-1495
                Affiliations
                [1 ] Laboratory of Food Biotechnology – Food Engineering, Institute of Science and Technology – UFVJM – Diamantina, Minas Gerais, Brazil
                Article
                10.1080/10408398.2019.1575791
                30740985
                88f94998-2b8e-4474-823d-8b303a4e8083
                © 2020
                History

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