UPARSE: highly accurate OTU sequences from microbial amplicon reads.

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Abstract

Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with ≤1% incorrect bases in artificial microbial community tests, compared with >3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community.

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Journal

Journal ID (iso-abbrev): Nat Methods

Title: Nature methods

Publisher: Springer Science and Business Media LLC

ISSN (Electronic): 1548-7105

ISSN (Print): 1548-7091

Publication date (Electronic): Oct 2013

Volume: 10

Issue: 10

Affiliations

[1 ] Independent Investigator, Tiburon, California, USA.

Article

Publisher Item ID: nmeth.2604

DOI: 10.1038/nmeth.2604

PubMed ID: 23955772

SO-VID: 87b1ec0f-935d-4bd2-bdc9-b1f183964ee2

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