In order to determine the Sterigmatocystin (ST) contaminated in grains by indirect competitive enzyme link immune sorbent assays (IC-ELISA), 1580 samples including wheat, corn and rice were taken from 12 provinces located in East-north, west-north, west-south, east and central China. Results showed that the average contaminated content of ST in wheat, corn and rice was 68.9 ug/kg, 32.2 ug/kg and 13.9 ug/kg respectively. Also, the contaminated rate was 98%, 89% and 72% respectively. The contaminated content and rate of ST was highest in wheat followed by corn and the rice ranged in third that was statistically highly significant. Severity of contamination was different in different geo-regions with higher in east China and Western China that in north-east China, central China and south-western China that were statistically highly significant.