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      Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein

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          Abstract

          A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum α-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA). A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents, less total assay time, and better linearity, recovery, precision, sensitivity and validity. AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA, and the results were compared with commercial electrochemiluminescence immunoassay (ECLIA) kit. The correlation coefficient between MPs-CLEIA and ELISA was obtained with R 2=0.6703; however, the correlation between MPs-CLEIA and ECLIA ( R 2=0.9582) was obviously better than that between colorimetric ELISA and ECLIA ( R 2=0.6866).

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          Management of hepatocellular carcinoma.

          Jordi Bruix, Morris Sherman (2005)
          Article 0 comments Cited 1332 times – based on 0 reviews     Review now
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            Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA).

            Rudolf M. Lequin (2005)
            This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully automated instruments in medical laboratories around the world use the immunoassay principle with an enzyme as the reporter label for routine measurements of innumerable analytes in patient samples. The impact of EIA/ELISA is reflected in the overwhelmingly large number of times it has appeared as a keyword in the literature since the 1970s. Clinicians and their patients, medical laboratories, in vitro diagnostics manufacturers, and worldwide healthcare systems owe much to these four inventors.
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              Magnetism and microfluidics.

              Nicole Pamme (2006)
              Magnetic forces are now being utilised in an amazing variety of microfluidic applications. Magnetohydrodynamic flow has been applied to the pumping of fluids through microchannels. Magnetic materials such as ferrofluids or magnetically doped PDMS have been used as valves. Magnetic microparticles have been employed for mixing of fluid streams. Magnetic particles have also been used as solid supports for bioreactions in microchannels. Trapping and transport of single cells are being investigated and recently, advances have been made towards the detection of magnetic material on-chip. The aim of this review is to introduce and discuss the various developments within the field of magnetism and microfluidics.
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                Author and article information

                Contributors
                Jin-Ming Lin
                Journal
                Journal ID (nlm-ta): J Pharm Anal
                Journal ID (iso-abbrev): J Pharm Anal
                Title: Journal of Pharmaceutical Analysis
                Publisher: Xi'an Jiaotong University
                ISSN (Print): 2095-1779
                ISSN (Electronic): 2214-0883
                Publication date PMC-release: 27 January 2012
                Publication date (Print): April 2012
                Publication date (Electronic): 27 January 2012
                Volume: 2
                Issue: 2
                Pages: 130-135
                Affiliations
                [a ]Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100029, China
                [b ]Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
                Author notes
                [* ]Corresponding author. Tel./fax: +86 10 62792343. jmlin@ 123456mail.tsinghua.edu.cn
                Article
                Publisher ID: S2095-1779(11)00042-6
                DOI: 10.1016/j.jpha.2011.10.001
                PMC ID: 5760820
                SO-VID: 85efeac8-bea1-4bd1-8581-b8241a376cdc
                Copyright © © 2011 Xi'an Jiaotong University
                License:

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

                History
                Date received : 18 August 2011
                Date accepted : 28 October 2011
                Categories
                Subject: Article

                Keywords: α-fetoprotein,hepatocellular carcinoma,chemiluminescence enzyme immunoassay,magnetic microparticles,colorimetric enzyme-linked immunosorbent assay
                Data availability:
                Keywords: α-fetoprotein, hepatocellular carcinoma, chemiluminescence enzyme immunoassay, magnetic microparticles, colorimetric enzyme-linked immunosorbent assay

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