The purpose of the current study was to determine the prevalence of depression and anxiety and their associated factors among Jordanian adolescents (n = 1,878) and Syrian adolescent refugees (n = 1,773) aged 12 to 17 years. The Center for Epidemiologic Studies-Depression Scale for Children (CES-DC), Patient Health Questionnaire-9-Modified, and Generalized Anxiety Disorder-7 questionnaire were used in this study. Approximately 27.2% of Jordanian adolescents and 28.3% of Syrian adolescent refugees had depression as assessed by the CES-DC. Among males, 17.6% of Jordanian adolescents and 19% of Syrian adolescent refugees had anxiety. Among females, 28.1% of Jordanian adolescents and 27.3% of Syrian adolescent refugees had anxiety. Overall, Jordanian adolescents and Syrian adolescent refugees had high prevalence of depression and anxiety. Establishing community-based mental health care in Jordan is crucial. [Journal of Psychosocial Nursing and Mental Health Services, 59(6), 23-30.].