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      Species identification of adult African blowflies (Diptera: Calliphoridae) of forensic importance

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          Abstract

          Necrophagous blowflies can provide an excellent source of evidence for forensic entomologists and are also relevant to problems in public health, medicine, and animal health. However, access to useful information about these blowflies is constrained by the need to correctly identify the flies, and the poor availability of reliable, accessible identification tools is a serious obstacle to the development of forensic entomology in the majority of African countries. In response to this need, a high-quality key to the adults of all species of forensically relevant blowflies of Africa has been prepared, drawing on high-quality entomological materials and modern focus-stacking photomicroscopy. This new key can be easily applied by investigators inexperienced in the taxonomy of blowflies and is made available through a highly accessible online platform. Problematic diagnostic characters used in previous keys are discussed.

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          The online version of this article (doi:10.1007/s00414-017-1654-y) contains supplementary material, which is available to authorized users.

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          Mobile Phones and Economic Development in Africa

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            Beyond barcoding: a mitochondrial genomics approach to molecular phylogenetics and diagnostics of blowflies (Diptera: Calliphoridae).

            Members of the Calliphoridae (blowflies) are significant for medical and veterinary management, due to the ability of some species to consume living flesh as larvae, and for forensic investigations due to the ability of others to develop in corpses. Due to the difficulty of accurately identifying larval blowflies to species there is a need for DNA-based diagnostics for this family, however the widely used DNA-barcoding marker, cox1, has been shown to fail for several groups within this family. Additionally, many phylogenetic relationships within the Calliphoridae are still unresolved, particularly deeper level relationships. Sequencing whole mt genomes has been demonstrated both as an effective method for identifying the most informative diagnostic markers and for resolving phylogenetic relationships. Twenty-seven complete, or nearly so, mt genomes were sequenced representing 13 species, seven genera and four calliphorid subfamilies and a member of the related family Tachinidae. PCR and sequencing primers developed for sequencing one calliphorid species could be reused to sequence related species within the same superfamily with success rates ranging from 61% to 100%, demonstrating the speed and efficiency with which an mt genome dataset can be assembled. Comparison of molecular divergences for each of the 13 protein-coding genes and 2 ribosomal RNA genes, at a range of taxonomic scales identified novel targets for developing as diagnostic markers which were 117-200% more variable than the markers which have been used previously in calliphorids. Phylogenetic analysis of whole mt genome sequences resulted in much stronger support for family and subfamily-level relationships. The Calliphoridae are polyphyletic, with the Polleninae more closely related to the Tachinidae, and the Sarcophagidae are the sister group of the remaining calliphorids. Within the Calliphoridae, there was strong support for the monophyly of the Chrysomyinae and Luciliinae and for the sister-grouping of Luciliinae with Calliphorinae. Relationships within Chrysomya were not well resolved. Whole mt genome data, supported the previously demonstrated paraphyly of Lucilia cuprina with respect to L. sericata and allowed us to conclude that it is due to hybrid introgression prior to the last common ancestor of modern sericata populations, rather than due to recent hybridisation, nuclear pseudogenes or incomplete lineage sorting. Copyright © 2012 Elsevier B.V. All rights reserved.
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              The role of non-biting flies in the epidemiology of human infectious diseases

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                Author and article information

                Contributors
                lena@die-lutzens.de
                kirstin.williams@durban.gov.za
                M.Villet@ru.ac.za
                mfoneka2004@yahoo.com
                szpila@umk.pl
                Journal
                Int J Legal Med
                Int. J. Legal Med
                International Journal of Legal Medicine
                Springer Berlin Heidelberg (Berlin/Heidelberg )
                0937-9827
                1437-1596
                28 August 2017
                28 August 2017
                2018
                : 132
                : 3
                : 831-842
                Affiliations
                [1 ]ISNI 0000 0004 1936 9721, GRID grid.7839.5, Institute of Forensic Medicine, Goethe-University Frankfurt, ; Kennedyallee 104, D-60596 Frankfurt am Main, Germany
                [2 ]Entomology Department, Durban Natural Science Museum, Durban, South Africa
                [3 ]GRID grid.91354.3a, Southern African Forensic Entomology Research Laboratory, Department of Zoology and Entomology, , Rhodes University, ; Grahamstown, South Africa
                [4 ]ISNI 0000 0000 9156 2260, GRID grid.412960.8, Department of Zoology, , University of Uyo, ; Uyo, Nigeria
                [5 ]ISNI 0000 0001 0943 6490, GRID grid.5374.5, Faculty of Biology and Environmental Protection, , Nicolaus Copernicus University, ; Lwowska 1, 87-100 Toruń, Poland
                Article
                1654
                10.1007/s00414-017-1654-y
                5919996
                28849264
                83f053f5-11b6-43b6-b137-7b4889bec617
                © The Author(s) 2017

                Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

                History
                : 5 April 2017
                : 20 July 2017
                Funding
                Funded by: FundRef http://dx.doi.org/10.13039/501100004281, Narodowe Centrum Nauki;
                Award ID: 2015/17/B/NZ8/02453
                Award Recipient :
                Categories
                Original Article
                Custom metadata
                © Springer-Verlag GmbH Germany, part of Springer Nature 2018

                Law
                calliphoridae,blowflies,species identification,africa,forensic entomology
                Law
                calliphoridae, blowflies, species identification, africa, forensic entomology

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