Low-cesium rice: mutation in OsSOS2 reduces radiocesium in rice grains

Abstract

In Japan, radiocesium contamination in foods has become of great concern and it is a primary issue to reduce grain radiocesium concentration in rice ( Oryza sativa L.). Here, we report a low-cesium rice mutant 1 ( lcs1) with the radiocesium concentration in grain about half that in the wild-type cultivar. Genetic analyses revealed that a mutation in OsSOS2, which encodes a serine/threonine-protein kinase required for the salt overly sensitive (SOS) pathway in plants, is responsible for the decreased cesium (Cs) concentrations in lcs1. Physiological analyses showed that Cs ⁺ uptake by lcs1 roots was significantly decreased under low-potassium (K ⁺) conditions in the presence of sodium (Na ⁺) (low K ⁺/Na ⁺). The transcript levels of several K ⁺ and Na ⁺ transporter genes, such as OsHAK1, OsHAK5, OsAKT1, and OsHKT2;1 were significantly down-regulated in lcs1 grown at low K ⁺/Na ⁺. The decreased Cs ⁺ uptake in lcs1 might be closely related to the lower expression of these genes due to the K ⁺/Na ⁺ imbalance in the lcs1 roots caused by the OsSOS2 mutation. Since the lcs1 plant had no significant negative effects on agronomic traits when grown in radiocesium-contaminated paddy fields, this mutant could be used directly in agriculture for reducing radiocesium in rice grains.