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      Prediction of bovine milk true protein content by mid-infrared spectroscopy Translated title: Estimativa do teor de proteína verdadeira do leite bovino por espectroscopia na região do infravermelho médio

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          Abstract

          The aim of this study was to estimate the concentration of milk true protein (TP) by mid-infrared absorbance method (MIR) in samples from bulk tank of dairy herds, and to determine the correlation between the results of TP of milk determined by Kjeldahl and MIR. Forty nine dairy herds were selected (17 Holstein, 6 Jersey and 26 Girolando) for monthly collections of samples from bulk tanks during the period of one year (284 samples). Fat, lactose, crude protein and total solids were firstly determined by MIR, and then analyzed for total and true protein by Kjeldahl method. The regression equation to estimate TP contents based on MIR crude protein determination was as follows: TP=0.0021+(1.0104xCP), where: TP is the content of true protein, CP is the crude protein content determined by the MIR method, and 0.0155 is the model error term.

          Translated abstract

          O objetivo deste estudo foi estimar o teor de proteína verdadeira (PV) do leite por meio de metodologia de espectroscopia na região do infravermelho médio (IVM), em amostras de tanque de rebanhos leiteiros comerciais, e determinar a correlação entre os resultados de proteína verdadeira do leite determinados pelo método de Kjeldahl e por IVM. Foram selecionados 49 rebanhos leiteiros (17 da raça Holandesa, seis da raça Jersey e 26 da raça Girolando) para coletas mensais de amostras de leite de tanque durante o período de um ano, totalizando 284 amostras analisadas. As amostras de leite foram analisadas inicialmente em relação aos teores de gordura, lactose, proteína bruta e sólidos totais por IVM, sendo em seguida analisadas quanto ao teor de PB e PV pelo método de Kjeldahl. A equação de regressão para estimativa do teor de PV com base nos teores de proteína bruta foi a seguinte: PV=0,0021+(1,0104xPB); em que: PV é o teor de proteína verdadeira estimado; PB é o teor de proteína bruta pelo método IVM e 0,0155 é o erro apresentado pelo modelo.

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          Determination of protein concentration in raw milk by mid-infrared fourier transform infrared/attenuated total reflectance spectroscopy.

          This study investigates the potential use of attenuated total reflectance spectroscopy in the mid-infrared range for determining protein concentration in raw cow milk. The determination of protein concentration is based on the characteristic absorbance of milk proteins, which includes 2 absorbance bands in the 1500 to 1700 cm(-1) range, known as the amide I and amide II bands, and absorbance in the 1060 to 1100 cm(-1) range, which is associated with phosphate groups covalently bound to casein proteins. To minimize the influence of the strong water band (centered around 1640 cm(-1)) that overlaps with the amide I and amide II bands, an optimized automatic procedure for accurate water subtraction was applied. Following water subtraction, the spectra were analyzed by 3 methods, namely simple band integration, partial least squares (PLS) and neural networks. For the neural network models, the spectra were first decomposed by principal component analysis (PCA), and the neural network inputs were the spectra principal components scores. In addition, the concentrations of 2 constituents expected to interact with the protein (i.e., fat and lactose) were also used as inputs. These approaches were tested with 235 spectra of standardized raw milk samples, corresponding to 26 protein concentrations in the 2.47 to 3.90% (weight per volume) range. The simple integration method led to very poor results, whereas PLS resulted in prediction errors of about 0.22% protein. The neural network approach led to prediction errors of 0.20% protein when based on PCA scores only, and 0.08% protein when lactose and fat concentrations were also included in the model. These results indicate the potential usefulness of Fourier transform infrared/attenuated total reflectance spectroscopy for rapid, possibly online, determination of protein concentration in raw milk.
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            Calibration of Infrared Milk Analyzers: Modified Milk Versus Producer Milk

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              Official methods of analysis

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                Author and article information

                Journal
                cr
                Ciência Rural
                Cienc. Rural
                Universidade Federal de Santa Maria (Santa Maria, RS, Brazil )
                0103-8478
                1678-4596
                August 2011
                : 41
                : 8
                : 1472-1474
                Affiliations
                [01] São Paulo SP orgnameUniversidade de São Paulo orgdiv1Faculdade de Medicina Veterinária e Zootecnica orgdiv2Departamento de Medicina Veterinária Preventiva Brasil
                [02] Pirassununga SP orgnameUniversidade de São Paulo orgdiv1Faculdade de Medicina Veterinária e Zootecnica orgdiv2Departamento Nutrição e Produção Animal Brasil mveiga@ 123456usp.br
                [03] Piracicaba SP orgnameUniversidade de São Paulo orgdiv1Escola Superior de Agricultura Luiz de Queiroz orgdiv2Departamento de Zootecnia Brasil
                Article
                S0103-84782011000800028 S0103-8478(11)04100828
                80dfdadf-1191-464c-a0de-64abfa4ce7a3

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 29 June 2010
                : 21 April 2011
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 11, Pages: 3
                Product

                SciELO Brazil

                Categories
                Animal Production

                determinação de proteína,composição do leite,vaca leiteira,protein determination,milk composition,dairy cow

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