17
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Functional regulation of Q by microRNA172 and transcriptional co‐repressor TOPLESS in controlling bread wheat spikelet density

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Summary

          Bread wheat ( Triticum aestivum) spike architecture is an important agronomic trait. The Q gene plays a key role in the domestication of bread wheat spike architecture. However, the regulatory mechanisms of Q expression and transcriptional activity remain largely unknown. In this study, we show that overexpression of bread wheat tae‐miR172 caused a speltoid‐like spike phenotype, reminiscent of that in wheat plants with the q gene. The reduction in Q transcript levels in the tae‐miR172 overexpression transgenic bread wheat lines suggests that the Q expression can be suppressed by tae‐miR172 in bread wheat. Indeed, our RACE analyses confirmed that the Q mRNA is targeted by tae‐miR172 for cleavage. According to our analyses, the Q protein is localized in nucleus and confers transcriptional repression activity. Meanwhile, the Q protein could physically interact with the bread wheat transcriptional co‐repressor TOPLESS (Ta TPL). Specifically, the N‐terminal ethylene‐responsive element binding factor‐associated amphiphilic repression ( EAR) ( LDLNVE) motif but not the C‐terminal EAR ( LDLDLR) motif of Q protein mediates its interaction with the CTLH motif of Ta TPL. Moreover, we show that the N‐terminal EAR motif of Q protein is also essentially required for the transcriptional repression activity of Q protein. Taken together, we reveal the functional regulation of Q protein by tae‐miR172 and transcriptional co‐repressor Ta TPL in controlling the bread wheat spike architecture.

          Related collections

          Most cited references20

          • Record: found
          • Abstract: found
          • Article: found
          Is Open Access

          psRNATarget: a plant small RNA target analysis server

          Plant endogenous non-coding short small RNAs (20–24 nt), including microRNAs (miRNAs) and a subset of small interfering RNAs (ta-siRNAs), play important role in gene expression regulatory networks (GRNs). For example, many transcription factors and development-related genes have been reported as targets of these regulatory small RNAs. Although a number of miRNA target prediction algorithms and programs have been developed, most of them were designed for animal miRNAs which are significantly different from plant miRNAs in the target recognition process. These differences demand the development of separate plant miRNA (and ta-siRNA) target analysis tool(s). We present psRNATarget, a plant small RNA target analysis server, which features two important analysis functions: (i) reverse complementary matching between small RNA and target transcript using a proven scoring schema, and (ii) target-site accessibility evaluation by calculating unpaired energy (UPE) required to ‘open’ secondary structure around small RNA’s target site on mRNA. The psRNATarget incorporates recent discoveries in plant miRNA target recognition, e.g. it distinguishes translational and post-transcriptional inhibition, and it reports the number of small RNA/target site pairs that may affect small RNA binding activity to target transcript. The psRNATarget server is designed for high-throughput analysis of next-generation data with an efficient distributed computing back-end pipeline that runs on a Linux cluster. The server front-end integrates three simplified user-friendly interfaces to accept user-submitted or preloaded small RNAs and transcript sequences; and outputs a comprehensive list of small RNA/target pairs along with the online tools for batch downloading, key word searching and results sorting. The psRNATarget server is freely available at http://plantgrn.noble.org/psRNATarget/.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development.

            X. Chen (2004)
            Plant microRNAs (miRNAs) show a high degree of sequence complementarity to, and are believed to guide the cleavage of, their target messenger RNAs. Here, I show that miRNA172, which can base-pair with the messenger RNA of a floral homeotic gene, APETALA2, regulates APETALA2 expression primarily through translational inhibition. Elevated miRNA172 accumulation results in floral organ identity defects similar to those in loss-of-function apetala2 mutants. Elevated levels of mutant APETALA2 RNA with disrupted miRNA172 base pairing, but not wild-type APETALA2 RNA, result in elevated levels of APETALA2 protein and severe floral patterning defects. Therefore, miRNA172 likely acts in cell-fate specification as a translational repressor of APETALA2 in Arabidopsis flower development.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              The Jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect Jasmonate-regulated stamen development in Arabidopsis.

              The Arabidopsis thaliana F-box protein CORONATINE INSENSITIVE1 (COI1) perceives jasmonate (JA) signals and subsequently targets the Jasmonate-ZIM domain proteins (JAZs) for degradation by the SCF(COI1)-26S proteasome pathway to mediate various jasmonate-regulated processes, including fertility, root growth, anthocyanin accumulation, senescence, and defense. In this study, we screened JAZ-interacting proteins from an Arabidopsis cDNA library in the yeast two-hybrid system. MYB21 and MYB24, two R2R3-MYB transcription factors, were found to interact with JAZ1, JAZ8, and JAZ11 in yeast and in planta. Genetic and physiological experiments showed that the myb21 myb24 double mutant exhibited defects specifically in pollen maturation, anther dehiscence, and filament elongation leading to male sterility. Transgenic expression of MYB21 in the coi1-1 mutant was able to rescue male fertility partially but unable to recover JA-regulated root growth inhibition, anthocyanin accumulation, and plant defense. These results demonstrate that the R2R3-MYB transcription factors MYB21 and MYB24 function as direct targets of JAZs to regulate male fertility specifically. We speculate that JAZs interact with MYB21 and MYB24 to attenuate their transcriptional function; upon perception of JA signal, COI1 recruits JAZs to the SCF(COI1) complex for ubiquitination and degradation through the 26S proteasome; MYB21 and MYB24 are then released to activate expression of various genes essential for JA-regulated anther development and filament elongation.
                Bookmark

                Author and article information

                Contributors
                sunjiaqiang@caas.cn
                Journal
                Plant Biotechnol J
                Plant Biotechnol. J
                10.1111/(ISSN)1467-7652
                PBI
                Plant Biotechnology Journal
                John Wiley and Sons Inc. (Hoboken )
                1467-7644
                1467-7652
                09 August 2017
                February 2018
                : 16
                : 2 ( doiID: 10.1111/pbi.2018.16.issue-2 )
                : 495-506
                Affiliations
                [ 1 ] National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing China
                Author notes
                [*] [* ] Correspondence (Tel +86 10 82107842; fax +86 10 82105828; email sunjiaqiang@ 123456caas.cn )
                [†]

                These authors contributed equally to this work.

                Article
                PBI12790
                10.1111/pbi.12790
                5787848
                28703466
                7f3f424b-9151-41fc-9f03-516c8bbed842
                © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

                This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

                History
                : 31 March 2017
                : 23 June 2017
                : 04 July 2017
                Page count
                Figures: 10, Tables: 0, Pages: 12, Words: 8252
                Funding
                Funded by: Ministry of Agriculture of China
                Award ID: 2016ZX08009003–003
                Funded by: National Key Research and Development Program of China
                Award ID: 2016YFD0100302
                Funded by: Institute of Crop Science
                Funded by: Chinese Academy of Agricultural Sciences (CAAS)
                Funded by: Agricultural Science and Technology Innovation Program of CAAS
                Funded by: Youth Talent Plan of CAAS
                Categories
                Research Article
                Research Articles
                Custom metadata
                2.0
                pbi12790
                February 2018
                Converter:WILEY_ML3GV2_TO_NLMPMC version:version=5.3.1.2 mode:remove_FC converted:29.01.2018

                Biotechnology
                wheat,spike architecture,mir172,q gene,transcriptional repression
                Biotechnology
                wheat, spike architecture, mir172, q gene, transcriptional repression

                Comments

                Comment on this article

                scite_
                0
                0
                0
                0
                Smart Citations
                0
                0
                0
                0
                Citing PublicationsSupportingMentioningContrasting
                View Citations

                See how this article has been cited at scite.ai

                scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

                Similar content333

                Cited by26

                Most referenced authors1,015