Mechanism of reduced vancomycin susceptibility conferred by walK mutation in community-acquired methicillin-resistant Staphylococcus aureus strain MW2.

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Abstract

Point mutations with unclear molecular mechanisms are often associated with vancomycin resistance in Staphylococcus aureus. Here, we observed that the walK (G223D) mutation caused decreased expression of genes associated with cell wall metabolism, decreased autolytic activity, thickened cell walls, and reduced vancomycin susceptibility. A phosphorylation assay showed that WalK (G223D) exhibited reduced autophosphorylation, which led to reduced phosphorylation of WalR. An electrophoretic mobility shift assay indicated that WalK (G223D)-phosphorylated WalR had a reduced capacity to bind to the atlA promoter.

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Journal

Journal ID (iso-abbrev): Antimicrob. Agents Chemother.

Title: Antimicrobial agents and chemotherapy

ISSN (Electronic): 1098-6596

ISSN (Print): 0066-4804

Publication date (Electronic): Feb 2015

Volume: 59

Issue: 2

Affiliations

[1 ] Department of Microbiology and Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, Anhui, China.

[2 ] Department of Microbiology and Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, Anhui, China Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui, China sunb@ustc.edu.cn.

Article

Publisher Item ID: AAC.04290-14

DOI: 10.1128/AAC.04290-14

PMC ID: 4335861

PubMed ID: 25451044

SO-VID: 7ddcbcb0-7fc5-4c24-85b4-39e8722b2dce

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