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      Chlorophyll fluorescence and other physiological parameters as indicators of waterlogging and shadow stress in lulo (Solanum quitoense var. septentrionale) seedlings Translated title: Parámetros de fluorescencia de la clorofila y otros parámetros fisiológicos como indicadores del estrés por anegamiento y sombrío en plántulas de lulo (Solanum quitoense var. septentrionale)

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          Abstract

          ABSTRACT Climate change has resulted in an increasing frequency of the phenomenon "La Niña," generating prolonged periods of waterlogging and low light. The objective of the present study was to evaluate the effects of two abiotic stresses: shading (65%) and waterlogging, and their interaction on fluorescence parameters of chlorophyll a in lulo (Solanum quitoense var. septentrionale) seedlings. A completely randomized design with a factorial arrangement was implemented. The first factor consisted of two levels of light (with and without shading). The second factor were four levels of duration of the waterlogging period (0, 3, 6 and 9 days), for a total of 8 treatments with three replicates. The response variables were recorded at 6, 12 and 18 days after the application of the waterlogging treatments began. Measurements of relative water content (RWC), electrolyte leakage, chlorophyll content and chlorophyll a fluorescence were recorded. The lulo plants appeared to be more susceptible to waterlogging than to shading, with a lower RWC when waterlogged 6 and 9 days, presenting damage at the level of photosystem II from day 3, causing a decrease in the chlorophyll content. The plants flooded under shading had a greater tolerance to this factor than those cultivated in full light. The techniques of quantification of the chlorophyll a fluorescence, especially the maximum quantum efficiency of the PSII, the effective photochemical quantum yield of PS II and the photochemical quenching were useful tools that characterized the lulo seedlings under stress conditions.

          Translated abstract

          RESUMEN El cambio climático trae como consecuencia el aumento en la frecuencia de fenómenos como los eventos "La Niña", generando periodos prolongados de anegamiento y sombrío. El objetivo del presente estudio fue evaluar los efectos de dos estreses abióticos sombrío (65%) y anegamiento y su interacción sobre parámetros de fluorescencia de la clorofila a en plántulas de lulo (Solanum quitoense var. septentrionale). Se implementó un diseño completamente al azar con un arreglo factorial. El primer factor consistió en dos niveles de sombrío (sin y con). El segundo factor fueron cuatro niveles (0, 3, 6 y 9 days) de duración del periodo de anegamiento, para un total de 8 tratamientos con tres repeticiones. Las variables de respuesta se registraron a los 6, 12 y 18 días después de iniciada la aplicación de los tratamientos de anegamiento. Se registraron medidas de contenido relativo de agua (CRA), fuga de electrolitos, contenido de clorofilas y fluorescencia de la clorofila a. Se encontró que las plantas de lulo son más susceptibles al anegamiento que al sombrío, evidenciado en una reducción del CRA en condiciones de anegamiento durante 6 y 9 días, presentando daño a nivel de fotosistema II a partir del día 3 y causando disminución en el contenido de cloro¬filas. Las plantas bajo sombrío presentaron mayor tolerancia al anegamiento en comparación a aquellas sin sombrío. Las técnicas de cuantificación de la fluorescencia de la clorofila a, especialmente la eficiencia máxima del PSII, la eficiencia real del PSII y el quenching fotoquímico, fueron una herramienta útil que permitió la caracterización de plántulas de lulo a condiciones de estrés.

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          Abiotic stress, the field environment and stress combination.

          Farmers and breeders have long known that often it is the simultaneous occurrence of several abiotic stresses, rather than a particular stress condition, that is most lethal to crops. Surprisingly, the co-occurrence of different stresses is rarely addressed by molecular biologists that study plant acclimation. Recent studies have revealed that the response of plants to a combination of two different abiotic stresses is unique and cannot be directly extrapolated from the response of plants to each of the different stresses applied individually. Tolerance to a combination of different stress conditions, particularly those that mimic the field environment, should be the focus of future research programs aimed at developing transgenic crops and plants with enhanced tolerance to naturally occurring environmental conditions.
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            Morphological, physiological and biochemical responses of plants to drought stress

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              How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll fluorescence decrease ratio RFd of leaves with the PAM fluorometer

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                Author and article information

                Journal
                rcch
                Revista Colombiana de Ciencias Hortícolas
                rev.colomb.cienc.hortic.
                Sociedad Colombiana de Ciencias Hotícolas, Universidad Pedagógica y Tecnológica de Colombia (Bogotá, Distrito Capital, Colombia )
                2011-2173
                December 2019
                : 13
                : 3
                : 325-335
                Affiliations
                [2] Bogota orgnameUniversidad Nacional de Colombia orgdiv1Faculty of Agricultural Sciences orgdiv2Department of Agronomy Colombia
                [3] Bogota orgnameUniversidad Nacional de Colombia orgdiv1Faculty of Agricultural Sciences orgdiv2Department of Agronomy Colombia
                [4] Bogota orgnameUniversidad Nacional de Colombia orgdiv1Faculty of Agricultural Sciences orgdiv2Department of Agronomy Colombia
                [5] Bogota Arauca orgnameUniversidad Nacional de Colombia Colombia
                [1] Bogota orgnameUniversidad Nacional de Colombia orgdiv1Faculty of Agricultural Sciences orgdiv2Department of Agronomy Colombia
                Article
                S2011-21732019000300325 S2011-2173(19)01300300325
                10.17584/rcch.2019vl3i3.10017
                7c97d559-e18e-41b0-8df7-e568763e044b

                This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

                History
                : 29 November 2019
                : 18 September 2019
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 51, Pages: 11
                Product

                SciELO Colombia

                Categories
                Section of fruits

                oxidative stress,light energy dissipation,electrolyte leakage,hipoxia, disipación de energía lumínica, fuga de electrolitos,hypoxia,estrés oxidativo

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