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      Assessment of technological options and economical feasibility for cyanophycin biopolymer and high-value amino acid production

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          Abstract

          Major transitions can be expected within the next few decades aiming at the reduction of pollution and global warming and at energy saving measures. For these purposes, new sustainable biorefinery concepts will be needed that will replace the traditional mineral oil-based synthesis of specialty and bulk chemicals. An important group of these chemicals are those that comprise N-functionalities. Many plant components contained in biomass rest or waste stream fractions contain these N-functionalities in proteins and free amino acids that can be used as starting materials for the synthesis of biopolymers and chemicals. This paper describes the economic and technological feasibility for cyanophycin production by fermentation of the potato waste stream Protamylasse™ or directly in plants and its subsequent conversion to a number of N-containing bulk chemicals.

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          Most cited references38

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          High cell-density culture of Escherichia coli.

          S Lee (1996)
          Escherichia coli is the most widely used prokaryotic system for the synthesis of heterologous proteins. Once an optimal expression system has been constructed, protein production can be enhanced by increasing the production of protein per cell per unit time (specific productivity), or by increasing the cell concentration per unit time (cell productivity). Various high cell-density culture (HCDC) techniques have been developed for growing recombinant and non-recombinant E. coli strains in fed-batch cultures at concentrations greater than 100 grams (dry cell weight) per liter. This article reviews the problems encountered in HCDC of E. coli, and discusses various solutions. Feeding strategies for HCDC of E. coli, and the results obtained using them, are also described.
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            Biomass in the manufacture of industrial products—the use of proteins and amino acids

            The depletion in fossil feedstocks, increasing oil prices, and the ecological problems associated with CO2 emissions are forcing the development of alternative resources for energy, transport fuels, and chemicals: the replacement of fossil resources with CO2 neutral biomass. Allied with this, the conversion of crude oil products utilizes primary products (ethylene, etc.) and their conversion to either materials or (functional) chemicals with the aid of co-reagents such as ammonia and various process steps to introduce functionalities such as -NH2 into the simple structures of the primary products. Conversely, many products found in biomass often contain functionalities. Therefore, it is attractive to exploit this to bypass the use, and preparation of, co-reagents as well as eliminating various process steps by utilizing suitable biomass-based precursors for the production of chemicals. It is the aim of this mini-review to describe the scope of the possibilities to generate current functionalized chemical materials using amino acids from biomass instead of fossil resources, thereby taking advantage of the biomass structure in a more efficient way than solely utilizing biomass for the production of fuels or electricity.
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              Polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants.

              Polyhydroxybutyrate (PHB), a high molecular weight polyester, is accumulated as a storage carbon in many species of bacteria and is a biodegradable thermoplastic. To produce PHB by genetic engineering in plants, genes from the bacterium Alcaligenes eutrophus that encoded the two enzymes required to convert acetoacetyl-coenzyme A to PHB were placed under transcriptional control of the cauliflower mosaic virus 35S promoter and introduced into Arabidopsis thaliana. Transgenic plant lines that contained both genes accumulated PHB as electron-lucent granules in the cytoplasm, nucleus, and vacuole; the size and appearance of these granules were similar to the PHB granules that accumulate in bacteria.
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                Author and article information

                Contributors
                hans.mooibroek@wur.nl
                Journal
                Appl Microbiol Biotechnol
                Applied Microbiology and Biotechnology
                Springer-Verlag (Berlin/Heidelberg )
                0175-7598
                1432-0614
                18 September 2007
                November 2007
                : 77
                : 2
                : 257-267
                Affiliations
                [1 ]Wageningen University and Research Centre, Chair of Valorization of Plant Production Chains, P.O. Box 17, NL-6700 AA Wageningen, The Netherlands
                [2 ]Department of Biobased Products, Agrotechnology and Food Sciences Group, Wageningen University and Research Centre, P.O. Box 17, NL-6700 AA Wageningen, The Netherlands
                [3 ]Easthouse Business Solutions B.V., Landschrijverlaan 35, NL-9451 KT Rolde, The Netherlands
                [4 ]AVEBE B.A., P.O. Box 15, NL-9640 AA Veendam, The Netherlands
                [5 ]Wageningen University and Research Centre, Plant Breeding, P.O. Box 386, NL-6700 AJ Wageningen, The Netherlands
                [6 ]Wageningen University and Research Centre, Molecular Biology, P.O. Box 8128, NL-6700 ET Wageningen, The Netherlands
                [7 ]Institute for Molecular Microbiology and Biotechnology, Westfälische Wilhelms-Universität Münster, Corrensstraße 3, 48149 Münster, Germany
                Article
                1178
                10.1007/s00253-007-1178-3
                2043089
                17876577
                7b9d9f11-89a2-48dc-a6c2-e28f8208d9f8
                © Springer-Verlag 2007
                History
                : 14 June 2007
                : 22 August 2007
                : 24 August 2007
                Categories
                Mini-Review
                Custom metadata
                © Springer-Verlag 2007

                Biotechnology
                plant waste, rest stream,bulk chemicals,cyanophycin,non-ribosomal,n-functionality,biorefinery,protamylasse

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