Practical guide to the diagnostics of ruminant gastrointestinal nematodes, liver fluke and lungworm infection: interpretation and usability of results

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

The diagnostics of ruminant parasites remains one of the cornerstones for parasite control best practices. Field veterinarians have several techniques at their disposal (fecal egg count, coproculture, FAMACHA®, plasma pepsinogen, ELISA- Ostertagia, ELISA- Fasciola, Baermann and ELISA-Lungworm) for the identification and/or quantification of gastrointestinal nematodes, lungworms and liver fluke infecting small ruminants and cattle. Each of these diagnostic tools has its own strengths and weaknesses and is more appropriate for a specific production operation and/or age of the animal (young and adults). This review focuses on the usability and interpretation of the results of these diagnostic tools. The most advanced technical information on sampling, storage, advantages and limitations of each tool for different types of production operations and animal categories is provided.

Graphical abstract

Related collections

Most cited references 87

Record: found
Abstract: found
Article: not found

World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance.

G.C. Coles, C. Bauer, F.H.M. BORGSTEEDE … (1992)

Methods have been described to assist in the detection of anthelmintic resistance in strongylid nematodes of ruminants, horses and pigs. Two tests are recommended, an in vivo test, the faecal egg count reduction test for use in infected animals, and an in vitro test, the egg hatch test for detection of benzimidazole resistance in nematodes that hatch shortly after embryonation. Anaerobic storage for submission of faecal samples from the field for use in the in vitro test is of value and the procedure is described. The tests should enable comparable data to be obtained in surveys in all parts of the world.

0 comments Cited 157 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans.

Giuseppe Cringoli, Laura Rinaldi, Maria Paola Maurelli … (2010)

Accurate diagnosis of parasitic infections is of pivotal importance for both individual patient management and population-based studies, such as drug efficacy trials and surveillance of parasitic disease control and elimination programs, in both human and veterinary public health. In this study, we present protocols for the FLOTAC basic, dual and double techniques, which are promising new multivalent, sensitive, accurate and precise methods for qualitative and quantitative copromicroscopic analysis. These various methods make use of the FLOTAC apparatus, a cylindrical device with two 5-ml flotation chambers, which allows up to 1 g of stool to be prepared for microscopic analysis. Compared with currently more widely used diagnostic methods for parasite detection in animals (e.g., McMaster and Wisconsin techniques) and humans (e.g., Kato-Katz and ether-based concentration techniques), the FLOTAC techniques show higher sensitivity and accuracy. All FLOTAC techniques can be performed on fresh fecal material as well as preserved stool samples, and require approximately 12-15 min of preparation time before microscopic analysis.

0 comments Cited 154 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals

Giuseppe Cringoli, Maria P Maurelli, Bruno Levecke … (2017)

Here the authors provide an extension of their original FLOTAC protocol, describing the Mini-FLOTAC technique, optimized to perform diagnosis of helminth and protozoan infections in humans and animals where centrifugation may not be practical.

0 comments Cited 96 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Gustavo Adolfo Sabatini: gustavo.sabatini@boehringer-ingelheim.com

Fernando de Almeida Borges: fernando.borges@ufms.br

Edwin Claerebout: Edwin.Claerebout@UGent.be

Leonor Sicalo Gianechini: sicalo.leonor@uga.edu

Johan Höglund: Johan.Hoglund@slu.se

Ray Matthew Kaplan: rkaplan@sgu.edu

Welber Daniel Zanetti Lopes: wdzlopes@hotmail.com

Sian Mitchell: sian.mitchell86@gmail.com

Laura Rinaldi: lrinaldi@unina.it

Georg von Samson-Himmelstjerna: samson.georg@fu-berlin.de

Pedro Steffan: steffan.pedro@gmail.com

Robert Woodgate: rob.woodgate@adelaide.edu.au

Journal

Journal ID (nlm-ta): Parasit Vectors

Journal ID (iso-abbrev): Parasit Vectors

Title: Parasites & Vectors

Publisher: BioMed Central (London )

ISSN (Electronic): 1756-3305

Publication date (Electronic): 8 February 2023

Publication date PMC-release: 8 February 2023

Publication date Collection: 2023

Volume: 16

Electronic Location Identifier: 58

Affiliations

[1 ]GRID grid.420061.1, ISNI 0000 0001 2171 7500, Boehringer Ingelheim Animal Health, ; Ingelheim am Rhein, Germany

[2 ]GRID grid.412352.3, ISNI 0000 0001 2163 5978, Universidade Federal de Mato Grosso do Sul, ; Campo Grande, Brazil

[3 ]GRID grid.5342.0, ISNI 0000 0001 2069 7798, Ghent University, ; Ghent, Belgium

[4 ]GRID grid.213876.9, ISNI 0000 0004 1936 738X, University of Georgia, ; Athens, USA

[5 ]GRID grid.6341.0, ISNI 0000 0000 8578 2742, Swedish University of Agricultural Sciences, ; Uppsala, Sweden

[6 ]GRID grid.412748.c, St. George’s University, ; St. George’s, West Indies Grenada

[7 ]GRID grid.411195.9, ISNI 0000 0001 2192 5801, Universidade Federal de Goias, ; Goiania, Brazil

[8 ]The former Animal and Plant Health Agency (APHA), Perth, UK

[9 ]GRID grid.4691.a, ISNI 0000 0001 0790 385X, University of Naples Federico II, ; Naples, Italy

[10 ]GRID grid.14095.39, ISNI 0000 0000 9116 4836, Freie Universität Berlin, ; Berlin, Germany

[11 ]Fiel & Steffan Consultores Asociados, Tandil, Argentina

[12 ]GRID grid.1010.0, ISNI 0000 0004 1936 7304, University of Adelaide, ; Roseworthy, Australia

Article

Publisher ID: 5680

DOI: 10.1186/s13071-023-05680-w

PMC ID: 9906602

PubMed ID: 36755300

SO-VID: 7b637576-413f-4e0c-a73f-8fbc0462fa88

License:

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

History

Date received : 7 November 2022

Date accepted : 21 January 2023

Custom metadata

ScienceOpen disciplines: Parasitology

Keywords: ruminants,parasite,diagnostics,fecal egg count,coproculture,famacha®,plasma pepsinogen,elisa-ostertagia,elisa-fasciola,baermann and elisa-lungworm

Data availability:

ScienceOpen disciplines: Parasitology

Keywords: ruminants, parasite, diagnostics, fecal egg count, coproculture, famacha®, plasma pepsinogen, elisa-ostertagia, elisa-fasciola, baermann and elisa-lungworm

Comments

Comment on this article

scite_

Smart Citations

Citing PublicationsSupportingMentioningContrasting

View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

Cited by 16

See all cited by

Most referenced authors 784

See all reference authors

Practical guide to the diagnostics of ruminant gastrointestinal nematodes, liver fluke and lungworm infection: interpretation and usability of results

Read this article at

Abstract

Graphical abstract

Related collections

Ticks and tick-borne pathogens

Most cited references 87

World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance.

FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans.

The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals

Author and article information

Contributors

Journal

Affiliations

Article

History

Categories

Custom metadata

Comments

Comment on this article

Similar content 114

Cited by 16

Most referenced authors 784